Dicamba and 2,4-D systems control many problematic weeds; however, drift to susceptible crops can be a concern in diverse production areas. Glufosinate-based systems are an alternative, but current recommended rates of glufosinate can result in variable control. Research was conducted in 2017 and 2018 to investigate the optimum time interval between sequential glufosinate applications and determine if the addition of glyphosate with glufosinate is beneficial for controlling Palmer amaranth and annual grasses in cotton. The interval between sequential applications (1, 3, 5, 7, 10, or 14 d or no second spray) was the whole plot and herbicide option (glufosinate or glufosinate plus glyphosate) was the subplot. Combined over herbicides, Palmer amaranth 15- to 20-cm tall (at four locations) was controlled 98% to 99% with sequential intervals of 1 to 7 d compared with 70% to 88% with intervals of 10 or 14 d. Lowest biomass weight and population densities were noted with 1- to 7-d intervals. Large crabgrass 15- to 20-cm tall (at five locations) was controlled 93% to 98% with glufosinate applications 3- to 7-d apart as compared with 76% to 81% with applications 10- to 14-d apart. Lowest biomass weights were observed with 1- to 7-d intervals. When glufosinate controlled grass less than 93%, adding glyphosate was beneficial. Neither interval between sequential applications nor herbicide option influenced cotton yield. Shorter time intervals between sequential application and including glyphosate can improve the effectiveness of a glufosinate-based system in managing Palmer amaranth and large crabgrass.

Agronomic crops engineered with resistance to 2,4-D or dicamba have been commercialized and widely adopted throughout the United States. Due to this, increased use of these herbicides in time and space has increased damage to sensitive crops. From 2014 to 2016, cucumber and cantaloupe studies were conducted in Tifton, GA to demonstrate how auxinic herbicides (2,4-D or dicamba), herbicide rate (1/75 or 1/250 field use), and application timing (26, 16, and 7 d before harvest (DBH) of cucumber; 54, 31, and 18 DBH of cantaloupe) influenced crop injury, growth, yield, and herbicide residue accumulation in marketable fruit. Greater visual injury, reductions in vine growth, and yield loss were observed at higher rates when herbicides were applied during early-season vegetative growth compared to late-season with fruit development. Dicamba was more injurious in cucumber while cantaloupe responded similarly to both herbicides. For cucumber, total fruit number and relative weights were reduced (16 to 19%) when either herbicide was applied at the 1/75 rate 26 DBH. Cantaloupe fruit weight was also reduced 21 and 10% when either herbicide was applied at the 1/75 rate 54 or 31 DBH, respectively. Residue analysis noted applications closer to harvest were more likely to be detectable in fruit than earlier applications. In cucumber, dicamba was detected at both rates when applied 7 DBH, while in cantaloupe it was detected at both rates when applied 18 or 31 DBH in 2016 and at the 1/75 rate applied 18 or 31 DBH in 2014. Detectable amounts of 2,4-D were not observed in cucumber but were detected in cantaloupe when applied at either rate 18 or 31 DBH. While early season injury will more likely reduce cucumber or cantaloupe yields, the quantity of herbicide residue detected will be most influenced by the time interval between the off-target incident and sampling.

The United States produced $1.28 billion worth of peanuts in 2019 of which Georgia produced 51% of the total production (USDA-NASS 2021). Peanut is susceptible to weed competition due to slow canopy establishment, prostrate growth habit, and wide critical period for weed control from 3 to 8 weeks after planting (Burke et al. 2007; Everman et al. 2008). Georgia-06G is the dominant peanut cultivar planted in the southeast and in 2020, 87% of the acres grown for certified peanut seed available for sale to growers was Georgia-06G (Anonymous, 2020a). Peanut is commonly in rotation with cotton in the region and therefore, similar weed issues between these systems persist. This includes Palmer amaranth ( Amaranthus palmeri S. Watson) which has been documented to be resistant to multiple herbicide modes of action making its control difficult (Heap 2021). To minimize yield loss from weeds, preemergence (PRE) herbicides are frequently used in peanut to inhibit weed germination and provide residual weed control (Grichar et al. 2001). In response to resistance issues, producers have continued to integrate PRE herbicides into their herbicide programs to minimize weed emergence.

Nutsedge species are problematic in plastic-mulched vegetable production because of the weed’s rapid reproduction and ability to penetrate the mulch. Vegetable growers rely heavily on halosulfuron to manage nutsedge species; however, the herbicide cannot be applied over mulch before vegetable transplanting due to potential crop injury. This can be problematic when multiple crops are produced on a single mulch installation. Field experiments were conducted to determine the response of broccoli, cabbage, squash, and watermelon to halosulfuron applied on top of mulch prior to transplanting. Halosulfuron at 80 g ai ha−1 was applied 21, 14, 7, and 1 d before planting (DBP), and 160 g ai ha−1 was applied 21 DBP. In all experiments, extending the interval between halosulfuron application and planting reduced crop injury. For squash and watermelon, visual injury, plant diameters/vine runner lengths, marketable fruit weights, and postharvest plant biomass resulted in similar values when applying 80 g ha−1 21 DBP and with the nontreated weed-free control. Reducing this interval increased injury for both crops. Visual crop injury and yield reductions up to 40% occurred, with halosulfuron applied 14, 7, or 1 DBP in squash and 1 DBP in watermelon. Broccoli and cabbage showed greater sensitivity, with injury and plant diameter reductions greater than 15%, even with halosulfuron applied at 80 g ha−1 21 DBP. Experimental results confirm that halosulfuron binds to plastic mulch, remains active, and is slowly released from the mulch over a substantial period, during rainfall or overhead irrigation events. Extending the plant-back interval to at least 21 d before transplanting did overcome squash and watermelon injury concerns with halosulfuron at 80 g ha−1, but not broccoli and cabbage. Applying halosulfuron over mulch to control emerged nutsedge before planting squash and watermelon would be beneficial if adequate rainfall or irrigation and appropriate intervals between application and planting are implemented.

The efficacy of auxinic herbicides, a valuable weed control tool for growers worldwide, has been shown to vary with the time of day in which applications are made. However, little is known about the mechanisms causing this phenomenon. Investigating the differential in planta behavior of these herbicides across different times of application may grant an ability to advise which properties of auxinic herbicides are desirable when applications must be made around the clock. Radiolabeled herbicide experiments demonstrated a likely increase in ATP-binding cassette subfamily B (ABCB)-mediated 2,4-D and dicamba transport in Palmer amaranth ( Amaranthus palmeri S. Watson) at simulated dawn compared to mid-day, as dose response models indicated that many orders of magnitude higher concentrations of N-1-naphthylphthalamic acid (NPA) and verapamil, respectively, are required to inhibit translocation by 50% at simulated sunrise compared to mid-day. Gas chromatographic analysis displayed that ethylene evolution in A . palmeri was higher when dicamba was applied during mid-day compared to sunrise. Furthermore, it was found that inhibition of translocation via 2,3,5-triiodobenzoic acid (TIBA) resulted in an increased amount of 2,4-D-induced ethylene evolution at sunrise, and the inhibition of dicamba translocation via NPA reversed the difference in ethylene evolution across time of application. Dawn applications of these herbicides were associated with increased expression of a putative 9-cis-epoxycarotenoid dioxygenase biosynthesis gene NCED1 , while there was a notable lack of trends observed across times of day and across herbicides with ACS1 , encoding 1-aminocyclopropane-1-carboxylic acid synthase. Overall, this research indicates that translocation is differentially regulated via specific protein-level mechanisms across times of application, and that ethylene release, a chief phytotoxic process involved in the response to auxinic herbicides, is related to translocation. Furthermore, transcriptional regulation of abscisic acid involvement in phytotoxicity and/or translocation are suggested.