Abstract:Plant essential oils (EOs) serve as a «safe» alternative to chemical or synthetic antimicrobials and antioxidants to struggle with the food borne pathogens or spoilage organisms, inhibiting lipid oxidation and thus extend shelf life of ground beef application. In our study, we investigated the effectiveness of thyme oil (Thymus vulgaris) at concentrations (1%, 1.5% and 2%) and cinnamon oil (Cinnamomum zeylanicum) at concentrations (0.5%, 1% and 1.5%) as natural preservatives as well as their ability to increas… Show more
“…So, the studied EOs bettered the microbial quality of beef sausage relative to the control formula. This result agrees with what is reported by El Adab & Hassouna, and Shaltout et al [31,6]. This decrease in the TBC values proved that thyme EO or clove EO has antimicrobial activities.…”
Section: Discussionsupporting
confidence: 93%
“…This test goals to find EOs that act as a "safe" substitute for artificial antioxidants and synthetic antimicrobials. Hence, nowadays, research is directed to detect EOs that can prevent the growth of both harmful and pathogenic organisms and inhibit fat oxidation for prolonging the shelf life of the food products [4,6].…”
Sausage is one of the well-liked foodstuffs in many areas. However, its quality is vulnerable to deteriorate owing to the lipid oxidation and microbial contamination. This research aimed to study the influence of thyme and clove essential oils (EOs) with or without nitrite on the chemical composition, microbial growth, and lipid oxidation rate in sausage during frozen storage-18°C for three months. The obtained results showed that the main component in clove EO and thyme EO is eugenol and thymol. Adding sodium nitrite to sausage resulted in declines in moisture and crude lipids and increases in protein and ash contents of sausage. Adding the tested EOs increased nitrite's effect on the chemical composition of sausage. Besides, these EOs lowered TBARS values, residual nitrite, and TBC in sausage. They also resulted in the rise of nitrite's efficacy in reducing TBARS values and TBC in sausage. Proteus was more sensitive than Klebsiella, whereas E. coli showed more resistance when adding nitrite or EO to sausage. The tested EOs increased the inhibitory influence of nitrite on Proteus, Klebsiella, Aspergillus niger, and Candida albicans in sausage. We concluded that the EOs of clove and thyme have antioxidant and antimicrobial efficacies in raw beef sausage during frozen storage. Also, they have a synergistic impact on nitrite. Therefore, it is suggested that these EOs, especially thyme, could be utilized to prolong shelf-life, prevent deterioration of sausage, and lessen the added nitrite's proportion to sausage for avoiding the formation of carcinogenic N-nitrosamines .
“…So, the studied EOs bettered the microbial quality of beef sausage relative to the control formula. This result agrees with what is reported by El Adab & Hassouna, and Shaltout et al [31,6]. This decrease in the TBC values proved that thyme EO or clove EO has antimicrobial activities.…”
Section: Discussionsupporting
confidence: 93%
“…This test goals to find EOs that act as a "safe" substitute for artificial antioxidants and synthetic antimicrobials. Hence, nowadays, research is directed to detect EOs that can prevent the growth of both harmful and pathogenic organisms and inhibit fat oxidation for prolonging the shelf life of the food products [4,6].…”
Sausage is one of the well-liked foodstuffs in many areas. However, its quality is vulnerable to deteriorate owing to the lipid oxidation and microbial contamination. This research aimed to study the influence of thyme and clove essential oils (EOs) with or without nitrite on the chemical composition, microbial growth, and lipid oxidation rate in sausage during frozen storage-18°C for three months. The obtained results showed that the main component in clove EO and thyme EO is eugenol and thymol. Adding sodium nitrite to sausage resulted in declines in moisture and crude lipids and increases in protein and ash contents of sausage. Adding the tested EOs increased nitrite's effect on the chemical composition of sausage. Besides, these EOs lowered TBARS values, residual nitrite, and TBC in sausage. They also resulted in the rise of nitrite's efficacy in reducing TBARS values and TBC in sausage. Proteus was more sensitive than Klebsiella, whereas E. coli showed more resistance when adding nitrite or EO to sausage. The tested EOs increased the inhibitory influence of nitrite on Proteus, Klebsiella, Aspergillus niger, and Candida albicans in sausage. We concluded that the EOs of clove and thyme have antioxidant and antimicrobial efficacies in raw beef sausage during frozen storage. Also, they have a synergistic impact on nitrite. Therefore, it is suggested that these EOs, especially thyme, could be utilized to prolong shelf-life, prevent deterioration of sausage, and lessen the added nitrite's proportion to sausage for avoiding the formation of carcinogenic N-nitrosamines .
“…Further, the antimicrobial effect of chitosan significantly (p < .05) decreased the TVBN concentration of T 3 as compared to T 1 and T 2 . A similar increasing trend in TVBN was also observed in minced meat treated with thyme and cinnamon essential oil (Shaltout, Thabet, & Koura, 2017). The results in the present study also corroborated with the reduction in microbial load, TBARS and TVBN value in fish patties coated with sunflower protein films incorporated with clove essential oil than control during refrigerated storage for 13 days as reported by Salgado, Lopez-Caballero, Gomez-Guillen, Mauri, and Montero (2013).…”
Section: Total Volatile Basic Nitrogen (Tvbn)supporting
The growing awareness about the harmful effects of synthetic preservatives and antioxidants is pressurizing the meat industry to look for the alternative use of natural preservatives and antioxidants in meat products. The younger generation is tending toward minimally processed foods preserved with natural ingredients rather than by using harmful synthetic preservatives which has given the rise to a new trend called "green consumerism" in the food industry (Imran et al., 2012). Microbial growth on food surfaces is a major cause of spoilage and edible coating and films in combination with natural preservatives can inhibit the surface microbial growth. The ability of any substance as coating material depends on its suitability as a barrier for the entry of microbes and its antioxidant as well as antimicrobial capacity (Kanatt, Rao, Chawla, & Sharma, 2013). Edible coatings offer various functions viz. decreased microbial growth, reduced fat oxidation, decreased moisture retardation and textural changes, reduced-fat uptake, reduction of undesirable chemical, and enzymatic changes and prevention of aroma loss (Dragich & Krochta, 2009;
“…Furthermore, the obtained results indicated that the best acceptability quality was attained at mixed group (thyme oil 0.4% +cinnamon oil 0.4%) then in thyme oil-treated minced beef samples, while slight improvement in acceptability of cinnamon oil minced beef samples as compared with control samples These results agreed with those obtained by Sasse et al, (2009) who reported that many herbs and spices as thyme contain antioxidant components that improve both color and flavor stability in meat. Also, Sallem-Amany et al, (2010) indicated that sensory properties of minced beef samples during cold storage (4°C)were enhanced by treatment minced beef by different concentrations of thyme oil (0.5%, 1%, 1.5%) compared to the untreated (control) samples and sample contain 1.5% thyme oil revealed best enhancement of sensory properties than sample contain 0.5% of the same oil, and those obtained by Shaltout et al,(2017) whose results were that meat samples containing 2% thyme oil and 1.5% cinnamon oil, demonstrated the highest enhancement of sensory attributes, while the samples treated with 1% of thyme and 0.5% of cinnamon oils demonstrated lower enhancement.…”
A total of 1800 g fresh minced beef were divided into 6 equal groups (3x100 g of each). Bacillus cereus was inoculated into each group with infective dose 29x10 7 cfu/g. The used natural oils were thyme oil (0.6% and 1%), cinnamon oil (0.6% and 1%) and mixture of them (0.4 % from each). The inoculated samples were stored at 4 o C in refrigerator until be used. The inoculated groups were examined every 24 hours for sensory examination (overall acceptability) and B. cereus count. The experiment was performed in triplicate. Thyme oil (0.6 and 1%) decreased count of B. cereus (cfu/g) from 2.2x10 8 (initial load) to 5.8x10 7 and 8.8 x10 6 with reduction percentages 73.63% and 96% on 5 th day and 6 th day of storage, respectively. Cinnamon oil (0.6 and 1 %) decreased count of B. cereus (cfu/g) to 9.8x10 7 and 6.1 x10 7 with reduction percentages 55.45% and 72.27% on 3 rd day and 5 th day of storage, respectively. Mixture of (thyme and cinnamon oils) decreased count of B. cereus (cfu/g) to 8.6x10 6 with reduction percentage 96.09%on 6 th day of storage. In control group B. cereus count increased from 2.2x10 8 (initial load) to 5.1x10 8 on 3 rd day. Furthermore, thyme oil (1%) and mixture treated minced beef showed overall acceptability till 6 th day of storage. In comparison, thyme oil (0.6%) and cinnamon oil (1%) showed overall acceptability till 5 th day. While, cinnamon oil (0.6%) and control group showed overall acceptability till 3 rd day. Generally, mixture of thyme and cinnamon oils (0.4% of each) proved to be more efficient than other concentrations in suppression of B. cereus growth in minced beef, Therefore, It is recommended to improve safety of the meat products.
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