Impact of soil tillage on the robustness of the genetic component of variation in phosphorus (P) use efficiency in barley (Hordeum vulgare L.)

George, Timothy S.; Brown, Lawrie; Newton, A. C.; Hallett, Paul D.; Sun, Benhua; Thomas, William; White, Philip J.

doi:10.1007/s11104-009-0209-5

Cited by 41 publications

(38 citation statements)

References 43 publications

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“…2A), which is required for normal plant growth (Reuter and Robison, 1997;George et al, 2011). Genotypic differences in P concentrations of shoots were observed at P75.…”

Section: Resultsmentioning

confidence: 99%

“…Considerable genetic variation in PAE and PUE has been reported in a wide range of barley and wheat genotypes (Gahoonia and Nielsen, 1996;Manske et al, 2001;Ozturk et al, 2005;Liao et al, 2008;George et al, 2011), but there have been few studies on the genetic variation in expression levels of Pi transporters and the relationships between the expression of Pi transporters and PAE or PUE (Ramaekers et al, 2010). To address this issue, we used barley, a diploid relative of hexaploid wheat, as a model system for winter cereals to study the functions of PHT1 genes involved in the acquisition and remobilization of Pi (Preuss et al, 2010(Preuss et al, , 2011.…”

mentioning

confidence: 99%

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Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

et al. 2011

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Genetic variation in phosphorus (P) efficiency exists among wheat (Triticum aestivum) and barley (Hordeum vulgare) genotypes, but the underlying mechanisms for the variation remain elusive. High-and low-affinity phosphate (Pi) PHT1 transporters play an indispensable role in P acquisition and remobilization. However, little is known about genetic variation in PHT1 gene expression and association with P acquisition efficiency (PAE) and P utilization efficiency (PUE). Here, we present quantitative analyses of transcript levels of high-and low-affinity PHT1 Pi transporters in four barley genotypes differing in PAE. The results showed that there was no clear pattern in the expression of four paralogs of the high-affinity Pi transporter HvPHT1;1 among the four barley genotypes, but the expression of a low-affinity Pi transporter, HvPHT1;6, and its close homolog HvHPT1;3 was correlated with the genotypes differing in PUE. Interestingly, the expression of HvPHT1;6 and HvPHT1;3 was correlated with the expression of HvIPS1 (for P starvation inducible; noncoding RNA) but not with HvIPS2, suggesting that HvIPS1 plays a distinct role in the regulation of the low-affinity Pi transporters. In addition, high PUE was found to be associated with high root-shoot ratios in low-P conditions, indicating that high carbohydrate partitioning into roots occurs simultaneously with high PUE. However, high PUE accompanying high carbon partitioning into roots could result in low PAE. Therefore, the optimization of PUE through the modification of low-affinity Pi transporter expression may assist further improvement of PAE for low-input agriculture systems.

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“…2A), which is required for normal plant growth (Reuter and Robison, 1997;George et al, 2011). Genotypic differences in P concentrations of shoots were observed at P75.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

et al. 2011

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“…No common QTLs could be detected between winter and spring types. However, the associations only just exceeded the threshold for detection, and George et al (2011) attribute this to the relatively small population sizes that were used for the study et al (1998) and/or limited genetic variation. QTLs for PUE in common bean were linked with the fin gene, which regulates determinism in this species (Cichy et al 2009).…”

Section: Genetics Of Puementioning

confidence: 92%

“…In a later study on a different wheat population, the effect of Vrn-A1 and Vrn-D1 were not detected, but it is unclear whether this population was segregating at these loci ; it would be useful to genotype the Vrn alleles of these parents. In barley, George et al (2011) attempted to identify QTLs for shoot P concentration in an association mapping panel of 120 barley genotypes comprising 56 winter and 64 spring types. No common QTLs could be detected between winter and spring types.…”

Section: Genetics Of Puementioning

confidence: 99%

Genetic approaches to enhancing phosphorus-use efficiency (PUE) in crops: challenges and directions

2013

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Abstract. Many soils have intrinsically low concentrations of available phosphorus (P), which is a major limitation to crop and pasture growth. Regular applications of P have underpinned agricultural productivity internationally, and fertiliser use now constitutes one of the largest variable input costs to farming. Globally, high-quality reserves of P are being depleted and price increases are likely in the future. In addition, the effects of P pollution on water quality are attracting legislative regulation. Hence, there is a need to improve P-use efficiency (PUE) in farming systems.Progress in improving PUE has been limited for several reasons, including: inconsistent definitions of PUE, inappropriate phenotyping, incomplete understanding of the controls of P uptake, lack of field validation, and little consideration of genotype Â environment interactions that affect the expression of PUE. With greater consideration of these limitations, the powerful array of molecular and genomic tools currently available promises considerable advances in developing more P-efficient crops. Stronger interaction between molecular science and the traditional disciplines of plant breeding, crop physiology, soil science, and agronomy will allow new opportunities to study genetic differences in PUE, bringing P-efficient crops closer to reality.

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“…Výsledky mohou mít analogii s poznatky George et al (2011), kteří u ozimého ječmene zaznamenali vyšší příjem P jak list, tak i kořeny. Mezi 2-řadými a 6-řadými odrůdami nebyl zjištěn prakticky žád-ný rozdíl, a to jak v obsahu PA (11,58 mg. ).…”

Section: Molecular Analyses Of "Lpa" Traitunclassified

Variability in phytic acid content in barley grain.

Vaculová¹,

Balounova²,

Kvasnička³

et al. 2012

Kvasny Prum

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V zrně ječmene se cca 65 % fosforu (P) nachází v podobě fytové kyseliny (PA) a jejích solí -fytátů, které negativně ovlivňují úroveň využitelnosti minerálních látek a dalších nutričně významných živin, podílejí se na minerálních deficiencích u lidí i ve výživě hospodář-ských zvířat a přispívají ke znečištění životního prostředí. Detekce a tvorba nových odrůd se změněným poměrem obou forem P, ve prospěch stravitelného fosfátu (Pi), je jedním ze způsobů řešení. Předložená práce se zabývala hodnocením variability obsahu PA a fosfátu (Pi) v zrně vybraných odrůd jarního a ozimého ječmene, genetických zdrojů a nových vlastních linií ječmene jarního, vytvořených hybridizací s tzv. "lpa" chemomutanty -donory nízkého obsahu PA (M422, M1070, M635 a M955). U jarního ječmene byla variabilita obsahu obou forem P ovlivněna ročníkovými vlivy a pouze při rozšíření hodnoceného souboru o lpa mutanty také genotypem. I přes vysokou ročníkovou proměnlivost zůstával u lpa mutantů přibližně stejný poměr Pi/PA (od 138,4 % u M422 po 772,1 % u M955), zatímco u standardních odrůd činil v průměru pouze 31,3 %. Porovnání ozimého a jarního ječmene prokázalo, že ozimé odrůdy mají silnou tendenci k vyšší kumulaci P v zrnu a ve formě PA (12,42 mg.g -1 vs. 10,94 mg.g -1 ). Rozpracování screeningové metody hodnocení obsahu volného P v zrnu pomocí kolorimetrického testu (KT) umožnilo provádět rozsáhlejší výběr materiálů s rozdílným obsahem PA a Pi. Mezi výsledky získanými aplikací metody KT a klasicky stanoveným obsahem Pi byla vypočtena silná kladná korelace (r = 0,85**, P≤0,01). Naopak využití existujících molekulárních markerů (SCAR marker ABC153) pro detekci kříženců se sníženým obsahem PA v zrnu se ukázalo jako velmi málo účinné. Křížením s lpa mutanty a následujícím výběrem za využití KT byly vytvořeny nové linie ječmene jarního se sní-ženým obsahem PA, zvýšeným podílem Pi a porovnatelným celkovým obsahem P v obilce, které mohou být využity ve šlechtění nebo dalším výzkumu. Zajímavým novým genovým zdrojem pro vývoj potravinářských odrůd ječmene se jeví nová linie KM2881.622.2.07, u které je zvýšený obsah volného P spojen s waxy charakterem škrobu (snížený podíl polysacharidu amylosy). Around 65 % of phosphorus (P) in barley grain is in the form of phytic acid (PA) and phytates (PA salts), both forms negatively affect the usability of mineral substances and other important nutrients, thus participating in mineral deficiencies in human as well as livestock nutrition, and contributing to the pollution of the environment. Detection and creation of new varieties with a changed rate of both P forms in favor of digestible phosphate (Pi) is one of possible solutions. The submitted study dealt with the evaluation of variability in contents of PA and Pi in grains of selected spring and winter barley varieties, genetic resources and new spring barley lines created by hybridization with so-called "lpa" chemo-mutants -donors of low PA content (M422, M1070, M635, and M955). Variability in the content of both P forms in spring barley was affected by year a...

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Impact of soil tillage on the robustness of the genetic component of variation in phosphorus (P) use efficiency in barley (Hordeum vulgare L.)

Cited by 41 publications

References 43 publications

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

Genetic approaches to enhancing phosphorus-use efficiency (PUE) in crops: challenges and directions

Variability in phytic acid content in barley grain.

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