Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats

Abstract

Six, nonpregnant, Barbari goats aged 4-5 years were selected for the study. For the first 6 days, the animals were kept in psychrometric chamber at thermoneutral temperature for 6 h each day to make them acclimated to climatic chamber. On the 7th day, the animals were exposed to 41 °C temperature for 3 h and then to 45 °C for the next 3 h. Cardinal physiological responses were measured, and blood samples (3 ml) were collected at 1-h interval during the heat exposure period and then once after 6 h of the heat exposure. The rectal temperature (RT) and respiratory rate (RR) increased significantly (P < 0.05) during the heat exposure compared to pre- and postexposure. The relative messenger RNA (mRNA) expression of heat shock protein (HSP)60, HSP70, and HSP90 increased significantly (P < 0.05) within 1 h after exposure to heat stress at 41 and 45 °C and decreased significantly (P < 0.05) in next 2 h but remain significantly (P < 0.05) elevated from preexposure. HSP105/110 relative mRNA expression level remained unchanged during the first 4 h, and thereafter, it increased significantly (P < 0.05) and reached the peak at 6 h. Relative protein expression pattern of HSPs during exposure to heat stress showed similar trend as observed for the relative mRNA expression. Given the response sensitivity and intensity of HSP genes to environmental stresses, HSP70 was found to be the most sensitive to temperature fluctuation, and it could be used as an important molecular biomarker to heat stress in animals.

Expression of TLR genes in Black Bengal goat (Capra hircus) during different seasons

“…In the present investigation, the mRNA expression of HSP70 was found to be significantly higher in animals below two years compared to the other age groups during both winter and summer season with the expression being higher in all age groups during summer season. Increased expression of HSP70 mRNA upon exposure to heat stress has been reported in caprine PBMCs (Sharma et al, 2013;Dangi et al, 2014) and bovine lymphocytes (Patir and Upadhyay, 2010;Mishra et al, 2010). In our studies, the mRNA expression of HSP60 in all age groups during summer season was found significantly (p < 0.05) higher and level increased with the age of animals.…”

“…The primers of HSP60, HSP70 and ribosomal protein S15a (RPS15A) were taken from published sequence (Dangi et al, 2012(Dangi et al, , 2014, and the primers for TLR1-10 and GAPDH were designed by the Fast PCR (Version: …”

“…The heat shock response confers transient thermal tolerance, in part due to the expression of heat shock proteins (HSPs). HSP70 play the most dominant role among all the HSPs in protecting cells from damage caused by acute thermal stress (Dangi et al, 2014). However, the expression of TLR genes during different season and the cross talk between HSP and Toll-like receptor (TLR) genes has not been studied in domestic animal so far.…”

Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus)

“…The primers of HSP70, HSP90, ribosomal protein S15a (RPS15A), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were taken from published sequence (Dangi et al 2012(Dangi et al , 2014a, and the primers of iNOS, eNOS, and cNOS were designed by the Fast PCR (version 6.2.73) software. The sequences and expected PCR product length are shown in Table 1.…”

Influência do estresse térmico no final da gestação de cabras: alterações da placenta e desempenho dos cabritos

“…Diferentemente aos resultados HSP70 por sua vez, foi aproximadamente 2 vezes mais expressa no tecido placentário do tratamento ET do que no tratamento CT, fato que fundamenta a hipótese do presente estudo, pois a exposição crônica ao calor desencadeou respostas celulares de proteção afim de evitar maiores danos à integridade e homeotasia das células. A diferença na expressão gênica da HSP70 entre os tratamentos, pode estar relacionada a maior sensibilidade que esta proteína tem às variações térmicas quando comparada às demais HSPs(DANGI et al 2014). No estudo realizado porBharati et al (2017) em bovinos submetidos ao estresse crônico por calor, foi observado aumento da expressão da HSP70 em função da temperatura e tempo de exposição ao calor de forma dependente, corroborando com os resultados obtidos no presente estudo.A redução significativa na expressão gênica da enzima 11β-HSD2 no tecido placentário das cabras ET em comparação as cabras CT, indica que a exposição crônica a elevadas Diferente ao que ocorre na placenta humana, onde há produção e secreção de CRH placentário(WANG et al 2014;PETRAGLIA et al 1987), no presente estudo não houve expressão gênica de CRH no tecido placentário das cabras de ambos os tratamentos CT e ET.Algumas pesquisas relatam que situações estressantes durante o período gestacional podem influenciar negativamente algumas atividades relacionadas ao comportamento maternofilial, como aumento das atividades agressivas e cuidados com a prole (KLAUS; SCHÖPPER; HUBER, 2013; PATIN et al 2002).…”