Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus)

Yadav, V.P.; Dangi, S.S.; Chouhan, V.S.; Gupta, Mahesh; Dangi, Saroj K.; Singh, Gyanendra; Maurya, V. P.; Kumar, Puneet; Sarkar, Mihir

doi:10.1007/s00484-015-1035-5

Cited by 32 publications

(16 citation statements)

References 37 publications

(34 reference statements)

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“…Concerning the adaptation to thermal stress, the annotated NOS1 (nitric-oxide synthase 1) contributes to the molecular function of nitric-oxide synthase activity (GO:0004517) and the nitric oxide biosynthetic process (GO:0006809). Yadav et al (2016) demonstrated significantly higher expression of different types of NOS ( P < 0.05) during summer and winter peaks, in goats, as compared to moderate season. Authors, therefore, indicated the possible involvement of the NOS family genes in ameliorating thermal stress and to maintain cellular integrity and homeostasis.…”

Section: Resultsmentioning

confidence: 90%

Natural Selection Footprints Among African Chicken Breeds and Village Ecotypes

et al. 2019

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Natural selection is likely a major factor in shaping genomic variation of the African indigenous rural chicken, driving the development of genetic footprints. Selection footprints are expected to be associated with adaptation to locally prevailing environmental stressors, which may include diverse factors as high altitude, disease resistance, poor nutrition, oxidative and heat stresses. To determine the existence of a selection footprint, 268 birds were randomly sampled from three indigenous ecotypes from East Africa (Rwanda and Uganda) and North Africa (Baladi), and two registered Egyptian breeds (Dandarawi and Fayoumi). Samples were genotyped using the chicken Affymetrix 600K Axiom ® Array. A total of 494,332 SNPs were utilized in the downstream analysis after implementing quality control measures. The intra-population runs of homozygosity (ROH) that occurred in >50% of individuals of an ecotype or in >75% of a breed were studied. To identify inter-population differentiation due to genetic structure, F ST was calculated for North- vs. East-African populations and Baladi and Fayoumi vs. Dandarawi for overlapping windows (500 kb with a step-size of 250 kb). The ROH and F ST mapping detected several selective sweeps on different autosomes. Results reflected selection footprints of the environmental stresses, breed behavior, and management. Intra-population ROH of the Egyptian chickens showed selection footprints bearing genes for adaptation to heat, solar radiation, ion transport and immunity. The high-altitude-adapted East-African populations’ ROH showed a selection signature with genes for angiogenesis, oxygen-heme binding and transport. The neuroglobin gene (GO:0019825 and GO:0015671) was detected on a Chromosome 5 ROH of Rwanda–Uganda ecotypes. The sodium-dependent noradrenaline transporter, SLC6A2 on a Chromosome 11 ROH in Fayoumi breed may reflect its active behavior. Inter-population F ST among Egyptian populations reflected genetic mechanisms for the Fayoumi resistance to Newcastle Disease Virus (NDV), while F ST between Egyptian and Rwanda–Uganda populations indicated the Secreted frizzled related protein 2, SFRP2 , (GO:0009314) on Chromosome 4, that contributes to melanogenic activity and most likely enhances the Dandarawi chicken adaptation to high-intensity of solar radiation in Southern Egypt. These results enhance our understanding of the natural selection forces role in shaping genomic structure for adaptation to the stressful African conditions.

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Section: Resultsmentioning

confidence: 90%

Natural Selection Footprints Among African Chicken Breeds and Village Ecotypes

et al. 2019

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“…In the present study, the HSP90 gene family of buffalo had AI greater than 65, indicating the stability of proteins at high temperatures. Similarly, the higher expression of HSP90 has been found in goat during the peak summer season, revealing the extent of thermostability of HSPs [32].…”

Section: Physiochemical Properties Of Hsp Gene Familiesmentioning

confidence: 78%

Genomic Identification, Evolution and Sequence Analysis of the Heat-Shock Protein Gene Family in Buffalo

Rehman

Nadeem

Javed

et al. 2020

Genes

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Heat-shock proteins (HSP) are conserved chaperones crucial for protein degradation, maturation, and refolding. These adenosine triphosphate dependent chaperones were classified based on their molecular mass that ranges between 10–100 kDA, including; HSP10, HSP40, HSP70, HSP90, HSPB1, HSPD, and HSPH1 family. HSPs are essential for cellular responses and imperative for protein homeostasis and survival under stress conditions. This study performed a computational analysis of the HSP protein family to better understand these proteins at the molecular level. Physiochemical properties, multiple sequence alignment, and phylogenetic analysis were performed for 64 HSP genes in the Bubalus bubalis genome. Four genes were identified as belonging to the HSP90 family, 10 to HSP70, 39 to HSP40, 8 to HSPB, one for each HSPD, HSPH1, and HSP10, respectively. The aliphatic index was higher for HSP90 and HSP70 as compared to the HSP40 family, indicating their greater thermostability. Grand Average of hydropathicity Index values indicated the hydrophilic nature of HSP90, HSP70, and HSP40. Multiple sequence alignment indicated the presence of highly conserved consensus sequences that are plausibly significant for the preservation of structural integrity of proteins. In addition, this study has expanded our current knowledge concerning the genetic diversity and phylogenetic relatedness of HSPs of buffalo with other mammalian species. The phylogenetic tree revealed that buffalo is more closely related to Capra hircus and distantly associated with Danio rerio. Our findings provide an understanding of HSPs in buffalo at the molecular level for the first time. This study highlights functionally important HSPs and indicates the need for further investigations to better understand the role and mechanism of HSPs.

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“…Therefore, the increased expressions of SOD and CAT genes are identified as a protective response to oxidative stress in animals (Akbarian et al, 2014). Further, NOS is identified as a potent gene involving in the thermal stress responses due to its crucial role in immune, circulatory system and signalling pathways in the animal body (Yadav et al, 2016). The NO initiates various cutaneous and vasodilator adaptive responses to local hyperthermic conditions.…”

Section: Biological Markers For Quantifying Heat Stress Response In Lmentioning

confidence: 99%

“…The NO initiates various cutaneous and vasodilator adaptive responses to local hyperthermic conditions. Moreover, higher production of NOS is essential for increased vasodilation of the skin during heat stress exposure (Yadav et al, 2016). Higher mRNA expression of NOS isoforms including inducible NOS, endothelial NOS and constitutitive NOS were reported in Barbari goats and Tharparkar cattle during hyperthermia (Collier et al, 2012).…”

Section: Biological Markers For Quantifying Heat Stress Response In Lmentioning

confidence: 99%

Review: Adaptation of animals to heat stress

Sejian

Bhatta

Gaughan

et al. 2018

Animal

319

206

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Livestock plays an important role in the global economy. Climate change effects are not only limited to crop production, but also affect livestock production, for example reduced milk yields and milk quality, reduced meat production and reduced fertility. Therefore, livestock-based food security is threatened in many parts of the world. Furthermore, multiple stressors are a common phenomenon in many environments, and are likely to increase due to climate change. Among these stresses, heat stress appears to be the major factor which negatively influences livestock production. Hence, it is critical to identify agro-ecological zone-specific climate resilient thermo-tolerant animals to sustain livestock production. Livestock responds to the changing environments by altering their phenotypic and physiological characters. Therefore, survivability of the animal often depends on its ability to cope with or adapt to the existing conditions. So to sustain livestock production in an environment challenged by climate change, the animals must be genetically suitable and have the ability to survive in diversified environments. Biological markers or biomarkers indicate the biological states or alterations in expression pattern of genes or state of protein that serve as a reference point in breeding for the genetic improvement of livestock. Conventionally, identification of animals with superior genetic traits that were economically beneficial was the fundamental reason for identifying biomarkers in animals. Furthermore, compared with the behavioural, morphological or physiological responses in animals, the genetic markers are important because of the possibility of finding a solution to animal adaptability to climate change.

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Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus)

Cited by 32 publications

References 37 publications

Natural Selection Footprints Among African Chicken Breeds and Village Ecotypes

Natural Selection Footprints Among African Chicken Breeds and Village Ecotypes

Genomic Identification, Evolution and Sequence Analysis of the Heat-Shock Protein Gene Family in Buffalo

Review: Adaptation of animals to heat stress

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