Impact of Reactive Oxygen and Reactive Nitrogen Species for Stem Cell Mobilization, Function and Cardiovascular Differentiation

Sauer, Heinrich; Wartenberg, Maria

doi:10.5772/15329

Embryonic Stem Cells: The Hormonal Regulation of Pluripotency and Embryogenesis 2011

DOI: 10.5772/15329

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Impact of Reactive Oxygen and Reactive Nitrogen Species for Stem Cell Mobilization, Function and Cardiovascular Differentiation

Heinrich Sauer¹,

Maria Wartenberg²

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Cited by 2 publications

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“…ROS and nitric oxide (NO) generation in stem cells could occur in response to transient changes in systemic redox balance and could initiate a feed forward cycle of ROS/NO generation and elaboration of a balanced anti-oxidative response system that might be the basis of stem cell proliferation, migration and differentiation. An increasing number of studies reported on the crucial role of ROS/NO on MSCs differentiation [28]. The neuronal differentiation of MSCs involved upregulation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and increased ROS generation [29].…”

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confidence: 99%

Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells

Widowati¹,

Sardjono²,

Wijaya³

et al. 2012

UCMS

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Objectives:Previous studies reported that proliferation and differentiation of stem cell are influenced by free radicals. Therefore, we conducted an investigation to know whether antioxidants, to our current interest, extract of Curcuma longa L. (ECL) and (-)-Epigallo catechin-3-gallate (EGCG), are playing role in differentiation and proliferation of adipose tissue-derived mesenchymal stem cells (AD-MSCs). Materials and Methods: ECL and AD-MSCs were prepared. Inhibitory concentration-median (IC-50 ) of ECL and EGCG were measured based on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity. To examine the effect of ECL and EGCG on proliferation and differentiation of AD-MSCs, ECL and EGCG in various concentrations were applied in culture of AD-MSCs for different period of time. Cell number was counted by trypan blue exclusion method. Differentiation of AD-MSCs into endothelial progenitor cells (EPCs) was analyzed based on following surface markers: cluster of differentiation 34 (CD34), CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2) with flow cytometer. Results: IC 50 of ECL and EGCG on DPPH scaven ing activity were 7.61 and 0.42 μg/mL, respectively. The highest proliferation rates were achieved by induction of ECL in concentrations of 1 g/mL, while induction of EGCG in concentration of 0.25 g/mL. ECL and EGCG enhanced differentiation of AD-MSCs into EPCs, marked by increasing expression of CD34, CD133 and VEGFR-2 at 4 incubation days. Conclusions: Our current results suggested that ECL and EGCG as antioxidant could enhance proliferation of AD-MSCs and differentiation of AD-MSCs into EPCs.

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confidence: 99%

Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells

Widowati¹,

Sardjono²,

Wijaya³

et al. 2012

UCMS

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Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

et al. 2015

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Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of suspension peripheral blood mononucleated stem cells (PBMNSCs) without resulting in loss in the differentiation potential. Mononucleated cells were isolated from both mice and human peripheral blood samples through gradient centrifugation and expanded in α-MEM, RPMI, MEM or DMEM supplemented with either NBCS or FBS. The suspension cells were then differentiated to osteoblast. Our data suggested that α-MEM supplemented with 10 % (v/v) NBCS gives the highest fold increase after 14 days of culture for both mice and human PBMNSCs, which were ~1.51 and ~2.01 times, respectively. The suspension PBMNSCs in the respective medium were also able to maintain osteoblast differentiation potential as supported by the significant increase in ALP specific activity. The cells are also viable during the differentiated states when using this media. All these data strongly suggested that α-MEM supplemented with 10 % NBCS is the best media for the expansion of both mouse and human suspension PBMNSCs.

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Impact of Reactive Oxygen and Reactive Nitrogen Species for Stem Cell Mobilization, Function and Cardiovascular Differentiation

Cited by 2 publications

References 144 publications

Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells

Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells

Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

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