Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

Ariffin, Shahrul Hisham Zainal; Rozali, Nur Akmal Mohamed; Wahab, Rohaya Megat Abdul; Senafi, Sahidan; Abidin, Intan Zarina Zainol

doi:10.1007/s10616-014-9819-8

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…Previous study demonstrated that alkaline phosphatase (ALP) activity and ability to form mineralized deposits were higher in MEM cultures than in DMEM cultures ( 31 ). Zainal et al and Salehinejad et al also revealed that α-MEM supported the expansion and differentiation of MSCs more strongly than DMEM ( 32 , 33 ). So, we decided to use MEM instead of DMEM in the optimized osteogenic and adipogenic medium.…”

Section: Discussionmentioning

confidence: 96%

Feline umbilical cord-derived mesenchymal stem cells: isolation, identification, and antioxidative stress role through NF-κB signaling pathway

Zhai

Zhao

et al. 2023

Front. Vet. Sci.

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At present, the differentiation potential and antioxidant activity of feline umbilical cord-derived mesenchymal stem cells (UC-MSCs) have not been clearly studied. In this study, feline UC-MSCs were isolated by tissue adhesion method, identified by flow cytometry detection of cell surface markers (CD44, CD90, CD34, and CD45), and induced differentiation toward osteogenesis and adipogenesis in vitro. Furthermore, the oxidative stress model was established with hydrogen peroxide (H2O2) (100 μM, 300 μM, 500 μM, 700 μM, and 900 μM). The antioxidant properties of feline UC-MSCs and feline fibroblasts were compared by morphological observation, ROS detection, cell viability via CCK-8 assay, as well as oxidative and antioxidative parameters via ELISA. The mRNA expression of genes related to NF-κB pathway was detected via quantitative real-time polymerase chain reaction, while the levels of NF-κB signaling cascade-related proteins were determined via Western Blot. The results showed that feline UC-MSCs highly expressed CD44 and CD90, while negative for CD34 and CD45 expression. Feline UC-MSCs cultured under osteogenic and adipogenic conditions showed good differentiation capacity. After being exposed to different concentrations of H2O2 for eight hours, feline UC-MSCs exhibited the significantly higher survival rate than feline fibroblasts. A certain concentration of H2O2 could up-regulate the activities of SOD2 and GSH-Px in feline UC-MSCs. The expression levels of p50, MnSOD, and FHC mRNA in feline UC-MSCs stimulated by 300 μM and 500 μM H2O2 significantly increased compared with the control group. Furthermore, it was observed that 500 μM H2O2 significantly enhanced the protein levels of p-IκB, IκB, p-p50, p50, MnSOD, and FHC, which could be reversed by BAY 11-7,082, a NF-κB signaling pathway inhibitor. In conclusion, it was confirmed that feline UC-MSCs, with good osteogenesis and adipogenesis abilities, had better antioxidant property which might be related to NF-κB signaling pathway. This study lays a foundation for the further application of feline UC-MSCs in treating the various inflammatory and oxidative injury diseases of pets.

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Section: Discussionmentioning

confidence: 96%

Feline umbilical cord-derived mesenchymal stem cells: isolation, identification, and antioxidative stress role through NF-κB signaling pathway

Zhai

Zhao

et al. 2023

Front. Vet. Sci.

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“…Presently, adult stem cells are the focus of most stem cell research because adult stem cells offer hope for stem cell therapy to treat disease in the future and ethical issues do not preclude its use. Adult stem cells can be obtained from a variety of sources such as peripheral blood (PB) ( Zainol Abidin et al., 2010 ; Yusof et al, 2011 ; Muniandy et al., 2013 ; Zainal Ariffin et al., 2016 ), dental pulp tissue ( Nur Akmal et al., 2014 ; Kermani et al, 2014 ; Hagar et al, 2021 ; Intan Zarina et al., 2022 ), bone marrow (BM), and periodontal ligament tissue ( Chong et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro osteoblast and osteoclast differentiation from stem cell: a systematic review of morphological assays and staining techniques

Zainal Ariffin,

Megat Abdul Wahab,

Abdul Razak

et al. 2024

PeerJ

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Background Understanding human stem cell differentiation into osteoblasts and osteoclasts is crucial for bone regeneration and disease modeling. Numerous morphological techniques have been employed to assess this differentiation, but a comprehensive review of their application and effectiveness is lacking. Methods Guided by the PRISMA framework, we conducted a rigorous search through the PubMed, Web of Science and Scopus databases, analyzing 254 articles. Each article was scrutinized against pre-defined inclusion criteria, yielding a refined selection of 14 studies worthy of in-depth analysis. Results The trends in using morphological approaches were identified for analyzing osteoblast and osteoclast differentiation. The three most used techniques for osteoblasts were Alizarin Red S (mineralization; six articles), von Kossa (mineralization; three articles) and alkaline phosphatase (ALP; two articles) followed by one article on Giemsa staining (cell morphology) and finally immunochemistry (three articles involved Vinculin, F-actin and Col1 biomarkers). For osteoclasts, tartrate-resistant acid phosphatase (TRAP staining) has the highest number of articles (six articles), followed by two articles on DAPI staining (cell morphology), and immunochemistry (two articles with VNR, Cathepsin K and TROP2. The study involved four stem cell types: peripheral blood monocyte, mesenchymal, dental pulp, and periodontal ligament. Conclusion This review offers a valuable resource for researchers, with Alizarin Red S and TRAP staining being the most utilized morphological procedures for osteoblasts and osteoclasts, respectively. This understanding provides a foundation for future research in this rapidly changing field.

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“…Several studies have shown that the composition of basal medias has a significant impact on the results of their experiments (Ariffin et al, 2016;Kim et al, 2015;Pirsko et al, 2018;Selenius et al, 2019;Tomoya Kawakami, 2016). However, culture media and supplementation remain a largely overlooked experimental parameter.…”

Section: Introductionmentioning

confidence: 99%

Co-occurrence of Cell Lines, Basal Media and Supplementation in the Biomedical Research Literature

Cox

McBeath

Harper

et al. 2020

Journal of Data and Information Science

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AbstractPurposeThe use of in vitro cell culture and experimentation is a cornerstone of biomedical research, however, more attention has recently been given to the potential consequences of using such artificial basal medias and undefined supplements. As a first step towards better understanding and measuring the impact these systems have on experimental results, we use text mining to capture typical research practices and trends around cell culture.Design/methodology/approachTo measure the scale of in vitro cell culture use, we have analyzed a corpus of 94,695 research articles that appear in biomedical research journals published in ScienceDirect from 2000–2018. Central to our investigation is the observation that studies using cell culture describe conditions using the typical sentence structure of cell line, basal media, and supplemented compounds. Here we tag our corpus with a curated list of basal medias and the Cellosaurus ontology using the Aho-Corasick algorithm. We also processed the corpus with Stanford CoreNLP to find nouns that follow the basal media, in an attempt to identify supplements used.FindingsInterestingly, we find that researchers frequently use DMEM even if a cell line's vendor recommends less concentrated media. We see long-tailed distributions for the usage of media and cell lines, with DMEM and RPMI dominating the media, and HEK293, HEK293T, and HeLa dominating cell lines used.Research limitationsOur analysis was restricted to documents in ScienceDirect, and our text mining method achieved high recall but low precision and mandated manual inspection of many tokens.Practical implicationsOur findings document current cell culture practices in the biomedical research community, which can be used as a resource for future experimental design.Originality/valueNo other work has taken a text mining approach to surveying cell culture practices in biomedical research.

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Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell

Cited by 5 publications

References 51 publications

Feline umbilical cord-derived mesenchymal stem cells: isolation, identification, and antioxidative stress role through NF-κB signaling pathway

Feline umbilical cord-derived mesenchymal stem cells: isolation, identification, and antioxidative stress role through NF-κB signaling pathway

Evaluation of in vitro osteoblast and osteoclast differentiation from stem cell: a systematic review of morphological assays and staining techniques

Co-occurrence of Cell Lines, Basal Media and Supplementation in the Biomedical Research Literature

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