Extract of Curcuma longa L. and (-)-Epigallo Catechin-3-Gallate Enhanced Proliferation of Adipose Tissue–derived Mesenchymal Stem Cells (AD-MSCs) and Differentiation of AD-MSCs into Endothelial Progenitor Cells

Abstract

Objectives:Previous studies reported that proliferation and differentiation of stem cell are influenced by free radicals. Therefore, we conducted an investigation to know whether antioxidants, to our current interest, extract of Curcuma longa L. (ECL) and (-)-Epigallo catechin-3-gallate (EGCG), are playing role in differentiation and proliferation of adipose tissue-derived mesenchymal stem cells (AD-MSCs). Materials and Methods: ECL and AD-MSCs were prepared. Inhibitory concentration-median (IC-50 ) of ECL and EGCG were measured based on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity. To examine the effect of ECL and EGCG on proliferation and differentiation of AD-MSCs, ECL and EGCG in various concentrations were applied in culture of AD-MSCs for different period of time. Cell number was counted by trypan blue exclusion method. Differentiation of AD-MSCs into endothelial progenitor cells (EPCs) was analyzed based on following surface markers: cluster of differentiation 34 (CD34), CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2) with flow cytometer. Results: IC 50 of ECL and EGCG on DPPH scaven ing activity were 7.61 and 0.42 μg/mL, respectively. The highest proliferation rates were achieved by induction of ECL in concentrations of 1 g/mL, while induction of EGCG in concentration of 0.25 g/mL. ECL and EGCG enhanced differentiation of AD-MSCs into EPCs, marked by increasing expression of CD34, CD133 and VEGFR-2 at 4 incubation days. Conclusions: Our current results suggested that ECL and EGCG as antioxidant could enhance proliferation of AD-MSCs and differentiation of AD-MSCs into EPCs.

Role of Herbal Extract in Stem Cell Development

“…Curcumin is able to prevent methylglyoxal-induced oxidative stress and apoptosis in mouse embryonic stem cells and blastocysts. 32 Based on previous report, it is known that IC 50 of CLE on 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity was 7.61 μg/ mL. The highest proliferation rates were induced by CLE at concentrations of 1 μg/mL.…”

“…Herbal extracts were now highly investigated due to its potential to induce cell proliferation. [31][32][33][34] The following are some potential herbal extracts in inducing stem cell proliferation.…”

“…In addition, EGCG induces differentiation of AD-MSCs into endothelial progenitor cells (EPCs) as well. 32 Later, it was found that EGCG, ECG, EGC and C of tea extract could induce differentiation of peripheral blood mononuclear cells (PB-MNCs) into peripheral blood-derived EPCs (PBEPCs). 31 The resulted PB-EPCs were confirmed by flow cytometry to detect EPCs membrane markers expressions (CD34, CD133, VEGFR-2).…”

Dental Mesenchymal Stem Cell: Its role in tooth development, types, surface antigens and differentiation potential

“…Not only that, they have enormous proliferative capacities and as the result MSCs are able to extend more than 10 passages in vitro without losing their original characteristics. [50][51][52] Previous studies show that the proliferation and differentiation of stem cells are due to the influence of free radicals. Another study show that antioxidants obtained from herbal extracts also play role in differentiation and proliferation of MSCs.…”

Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells

“…Indonesia with its wide biodiversities has plenty of resourceful natural materials those should be explored. Previously our team reported several potential extracts derived from plants including Ipomea batatas (1)(2)(3), Artocarpus altilis (4), Piper crocatum (5), Kleinhovia hospita (6), Artocarpus heterophyllus (7), Catharanthus roseus (8), Piper betle (9), Catharanthus roseus (9), Dendrophtoe petandra (9), Curcuma mangga (9), Curcuma longa (10), Ananas comosus (11), Brucea javanica (12,13), Nephelium lappaceum (14), Cucumis melo (15,16), Caesalpinia sappan (17) and Artocarpus elasticus (18). These extracts shows various activities, including osteoclastogenesis inhibition (1-3), apoptosis induction or proliferation Results inhibition on breast cancer cells (4,5,(7)(8)(9), antioxidant (6,14,16,18), proliferation and differentiation induction on stem cell (10,15), and apoptosis induction on human oral squamous cell carcinoma (12,13,16,17).…”