Role of Herbal Extract in Stem Cell Development

Sandra, Ferry

doi:10.21705/mcbs.v2i1.19

Cited by 6 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Herbal extract has been explored for various purposes, including for inhibiting cancer cell and inducing stem cell. (27,28) Cardiotoxicity of doxorubicin toxicity could be proceeded by an increasing amount of iron-mediated reactive oxygen species (ROS). Specifically, iron accumulates in the mitochondria of cardiac cells, causing tissue destruction and degradation.…”

Section: Discussionmentioning

confidence: 99%

Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer

et al. 2022

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Parkia speciosa seeds have been reported to have an anticancer property due to the presence of various antioxidant compounds. Since the potential uses of P. speciosa for the tongue cancer has not been clearly disclosed, we conducted a study to investigate anticancer properties of P. speciosa seed ethanol extract (PSSEE) as well as its effect on cardiac cells.METHODS: Tongue cancer rat model were treated with/without Doxorubicin and various concentrations of PSSEE. After treatment, tongue and heart samples were collected and processed further for histological examinations. Tongue epithelium thickness and damaged heart tissues was observed by HE staining, while tongue cancer cell proliferation was assessed by Ki-67 immunohistochemistry. Analyses were performed under an upright light microscope to measure tongue epithelium thickness, state of cancer cell proliferation, and degree of heart tissue damage.RESULTS: Addition of 400 mg/kg body weight (BW) PSSEE to 4.6 mg/kg BW Doxorubicin reduced the average tongue epithelial thickness and Ki-67+ cells number. Upon addition of PSSEE to Doxorubicin, the damage of heart tissue was reduced in a concentration dependent manner. Among all groups, the group of tongue cancer treated with 4.6 mg/kg BW Doxorubicin and 400 mg/kg BW PSSEE had the lowest percentage as well as the lowest degree of heart tissue damage.CONCLUSION: Since addition of PSSEE to Doxorubicin reduced epithelial thickness, number of Ki-67+ cells and heart tissue damage, PSSEE could be a potential co-chemotherapeutic agent for Doxorubicin toward tongue cancer.KEYWORDS: Parkia speciosa, Doxorubicin, tongue cancer, epithelial thickness, Ki-67, cardiotoxicity, co-chemotherapy

show abstract

Section: Discussionmentioning

confidence: 99%

Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The use of plants as an alternative medicine for some diseases in Indonesia has been carried out for generations. (1,2) Herbal medicines are also widely used to increase the immunity of the body, which are commonly referred to as immunomodulators. The ability of herbal medicine as an immunomodulator can be determined based on its secondary metabolite content.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Activity of Agarwood Aquilaria malaccensis Lamk. Leaf Extracts on Staphylococcus aureus-infected Macrophages in vitro

et al. 2022

View full text Add to dashboard Cite

BACKGROUND: Aquilaria malaccensis has been consumed as herbal medicine, and in vitro study showed that the leaf extract possesses high antioxidant activities. A brief preliminary study indicated that A. malaccensis showed a promising immunomodulatory activity when evaluated using latex beads. This current study aimed to evaluate the immunomodulatory activity of A. malaccensis leaf extract on the macrophage, which was challenged with pathogenic bacteria Staphylococcus aureus.METHODS: Bioactivity was determined by evaluating the phagocytic capacity of macrophages isolated from Mus musculus against S. aureus. First, the cytotoxicity of extracts on macrophages was evaluated using MTT assays, and the IC50 value was used to determine the dose of immunomodulatory assays. The highest toxicity was observed on chloroform extract with an IC50 value of 111.4 µg/mL. Therefore, the treatment was 100 and 50 µg/mL. Two parameters, including the phagocytic activity and phagocytic capacity of macrophages infected with S. aureus, were used to evaluate immunomodulatory activity. The analysis of variance was done at p<0.05 to determine the significant difference among treatments.RESULTS: Chloroform and ethanol extracts at a 50 µg/mL concentration showed the best results with the phagocytic activity of 82.33%±9.61% and 80.33±1.53%. The ethyl acetate showed lower phagocytic activities of 70.67±1.53. All extracts significantly increased phagocytic activity and phagocytic capacity, and the results differed significantly between negative and positive controls. Thin-layer chromatography indicated that the extract contained terpenoid, flavonoid, phenolic, and tannin.CONCLUSION: A. malaccensis leaf extracts showed immunomodulatory activity. Both chloroform and ethanol extracts showed comparable activity, while the ethyl acetate extract was lower. The extracts contained diverse bioactive compounds that may support activating macrophage cells for immunomodulatory activity.KEYWORDS: Aquilaria malaccensis, immunomodulator, phagocytosis, macrophages, Staphylococcus aureus

show abstract

“…37 UC-MSCs are used more often than dental pulp stem cells (DPSCs), though dental pulp has been considered as an attractive source of MSCs due to its high cell content and relatively low invasive cell isolation procedure. 17,33,[38][39][40] DPSCs are similar to ASCs in their immunomodulatory properties. 33 There are 3 types of stem cell transplantation, i.e.…”

Section: Introductionmentioning

confidence: 99%

Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review

Dini¹,

Nugraha²,

Revina³

et al. 2022

Mol Cell Biomed Sci

View full text Add to dashboard Cite

The experimental research on the use of mesenchymal stem cells (MSCs) for burn therapy has been published several times. However, current clinical procedure remains a challenging discussion. This systematic review assesses the safety and efficacy of administering mesenchymal stem cells (MSCs) to burns and determines the most effective source of MSCs for burn therapy. We reviewed several studies through PubMed, Google Scholar, Science Direct, and DOAJ online databases. PRISMA-P 2020 method was used based on inclusion and exclusion criteria that were re-selected through Joanna Briggs Institute (JBI) Critical Appraisal Tools. Results from 13 articles showed that MSCs are safe for burn therapy with minimal side effects/complications and have the potential to repair tissue and accelerate burn healing through several mechanisms. The treatment of MSCs in burns is influenced by donor characteristics and related to the severity and area of the burn. It can be concluded that the administration of MSCs is safe and effective in burn therapy. Keywords: burns, mesenchymal stem cells, therapeutic safety, therapeutic efficacy, wound healing

show abstract

Role of Herbal Extract in Stem Cell Development

Cited by 6 publications

References 17 publications

Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer

Parkia speciosa Seeds Ethanol Extract as Co-chemotherapeutic Agent for Doxorubicin Toward Tongue Cancer

Immunomodulatory Activity of Agarwood Aquilaria malaccensis Lamk. Leaf Extracts on Staphylococcus aureus-infected Macrophages in vitro

Safety and Efficacy of Mesenchymal Stem Cells in Burn Therapy: Systematic Review

Contact Info

Product

Resources

About