Impact of periodontal treatment on the RANKL/OPG ratio in crevicular fluid

Roldán, Andrés López; García-Giménez, José Luis; Illueca, Francisco Alpiste

doi:10.1371/journal.pone.0227757

Cited by 21 publications

(16 citation statements)

References 34 publications

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“…The pressure side, characterized by bone resorption, displays a high RANKL/OPG profile similar to that observed in chronic periodontitis, whereas the tension side, characterized by new bone formation, presents a RANKL < OPG profile similar to gingivitis sites (Menezes et al 2008). Accordingly, previous studies also described an increased RANKL/OPG ratio in periodontitis samples in comparison to healthy or gingivitis tissues, with increased RANKL levels associated with progressive tissue destruction (Bostanci et al 2007; Bi, Sun, et al 2019; Lopez Roldan et al 2020). Nevertheless, longitudinal prospective studies are required to further determine if a RANKL/OPG ratio comprises an effective biomarker of disease activity as described for other bone-related conditions.…”

Section: Lesion Progression Patterns: Evidence From Clinical and Labomentioning

confidence: 80%

Determinants of Periodontal/Periapical Lesion Stability and Progression

et al. 2020

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Periodontal and periapical lesions are infectious inflammatory osteolitytic conditions in which a complex inflammatory immune response mediates bone destruction. However, the uncertainty of a lesion’s progressive or stable phenotype complicates understanding of the cellular and molecular mechanisms triggering lesion activity. Evidence from clinical and preclinical studies of both periodontal and periapical lesions points to a high receptor activator of NF-κB ligand/osteoprotegerin (RANKL/OPG) ratio as the primary determinant of osteolytic activity, while a low RANKL/OPG ratio is often observed in inactive lesions. Proinflammatory cytokines directly modulate RANKL/OPG expression and consequently drive lesion progression, along with pro-osteoclastogenic support provided by Th1, Th17, and B cells. Conversely, the cooperative action between Th2 and Tregs subsets creates an anti-inflammatory and proreparative milieu associated with lesion stability. Interestingly, the trigger for lesion status switch from active to inactive can originate from an unanticipated RANKL immunoregulatory feedback, involving the induction of Tregs and a host response outcome with immunological tolerance features. In this context, dendritic cells (DCs) appear as potential determinants of host response switch, since RANKL imprint a tolerogenic phenotype in DCs, described to be involved in both Tregs and immunological tolerance generation. The tolerance state systemically and locally suppresses the development of exacerbated and pathogenic responses and contributes to lesions stability. However, immunological tolerance break by comorbidities or dysbiosis could explain lesions relapse toward activity. Therefore, this article will provide a critical review of the current knowledge concerning periodontal and periapical lesions activity and the underlying molecular mechanisms associated with the host response. Further studies are required to unravel the role of immunological responsiveness or tolerance in the determination of lesion status, as well as the potential cooperative and/or inhibitory interplay among effector cells and their impact on RANKL/OPG balance and lesion outcome.

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Section: Lesion Progression Patterns: Evidence From Clinical and Labomentioning

confidence: 80%

Determinants of Periodontal/Periapical Lesion Stability and Progression

et al. 2020

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“…RANKL induces maturation of osteoclasts, which are directly responsible for resorption of the dento-alveolar bone complex. Treating periodontal conditions was found to result in a significant reduction of RANKL levels in the gingival crevicular fluid, confirming the significance of RANKL in the inflammatory periodontal process [ 172 ]. In oral cancer, this amplified osteoclastic activity in PD supports the invasion of cancer cells into bone, which promotes their metastatic spread [ 173 ].…”

Section: Changes In the Periodontal Microenvironment That Contribute To The Pathogenesis Of Osccmentioning

confidence: 86%

The Crossroads of Periodontitis and Oral Squamous Cell Carcinoma: Immune Implications and Tumor Promoting Capacities

Elebyary

Barbour

Fine

et al. 2021

Front. Oral. Health

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Periodontitis (PD) is increasingly considered to interact with and promote a number of inflammatory diseases, including cancer. In the case of oral squamous cell carcinoma (OSCC) the local inflammatory response associated with PD is capable of triggering altered cellular events that can promote cancer cell invasion and proliferation of existing primary oral carcinomas as well as supporting the seeding of metastatic tumor cells into the gingival tissue giving rise to secondary tumors. Both the immune and stromal components of the periodontium exhibit phenotypic alterations and functional differences during PD that result in a microenvironment that favors cancer progression. The inflammatory milieu in PD is ideal for cancer cell seeding, migration, proliferation and immune escape. Understanding the interactions governing this attenuated anti-tumor immune response is vital to unveil unexplored preventive or therapeutic possibilities. Here we review the many commonalities between the oral-inflammatory microenvironment in PD and oral-inflammatory responses that are associated with OSCC progression, and how these conditions can act to promote and sustain the hallmarks of cancer.

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“…OPG, which plays an active role in the mechanism of bone destruction resulting from periodontal disease, also suppresses osteoclastic activity [ 35 ]. OPG generally increases in cases where the osteoclastic-osteoblastic cycle is intensely observed, such as during periodontal treatment, surgical procedures, and orthodontic applications [ 35 ]. The number of studies examining the effects of resin composites on OPG is negligible [ 11 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Flowable Composite Resins on Periodontal Health, Cytokine Levels, and Immunoglobulins

Peşkersoy

Oguzhan

Gürlek

2022

BioMed Research International

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Objective. This study investigated the effects of flowable resin composites (FCR) on the restoration of noncarious cervical lesions (NCCL) and their impact on periodontal tissues. Materials and Methods. 30 periodontally healthy patients were assigned into three groups randomly; group VF: self-adhering FCR, group NF: fluoride-releasing FCR, and group SF: microhybrid FCR. Gingival crevicular fluid (GCF) volume levels of osteoprotegerin (OPG), immunoglobulins (IgA, IgM), and interleukins (IL-1, IL-1β, and IL-10) in GCF were analyzed with ELISA tests. Clinical success rates were evaluated using USPHS criteria during the 12-month follow-up. Results. The GCF volume was increased mostly in group SF ( 1.34 ± 0.09 μ l ). While the titer of interleukin was increased in all groups, higher increases were observed in IL-1 and IL-1β in group NF (170.78 pg/ml and 39.35 pg/ml). Increased IL-10 was observed in group VF ( 14.33 ± 0.85 pg / ml ). IgA levels varied partially among all groups ( p > 0.05 ), and even IgM levels were elevated immediately after the restoration process but returned to normal on the 28th day ( p < 0.05 ). Group NF failed in most of the USPHS criteria, while the material group VF and group SF presented acceptable results except in the marginal adaptation criterion ( p < 0.05 ). Conclusions. Clinical efficacy of self-adhering FCR was found the best for restoration of NCCL while fluoride-releasing FCR stimulated the periodontal response and had negative effects on GCF volume, cytokine, and immunoglobulin levels.

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Impact of periodontal treatment on the RANKL/OPG ratio in crevicular fluid

Cited by 21 publications

References 34 publications

Determinants of Periodontal/Periapical Lesion Stability and Progression

Determinants of Periodontal/Periapical Lesion Stability and Progression

The Crossroads of Periodontitis and Oral Squamous Cell Carcinoma: Immune Implications and Tumor Promoting Capacities

The Effect of Flowable Composite Resins on Periodontal Health, Cytokine Levels, and Immunoglobulins

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