Impact of nano-selenium on nuclear maturation and genes expression profile of buffalo oocytes matured in vitro

El-Naby, Al-Shimaa Al-H H; Ibrahim, Sally; Hozyen, Heba F.; Sosa, Ahmed; Mahmoud, Karima Gh. M.; Farghali, Ahmed A.

doi:10.1007/s11033-020-05902-9

Cited by 7 publications

(10 citation statements)

References 43 publications

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“…They have found that supplementation of selenium in large-size or nano-particle forms improved buffalo oocytes maturation and the size of nanoparticles has an impact on this improvement. Furthermore, when compared to bulk-form selenium nanoparticles (40 nm) stimulate greater antioxidant gene expression (El-Naby et al, 2020). Nanopurification could be used to separate damaged sperm from intact and healthy sperm.…”

Section: Assisted Reproductive Technologymentioning

confidence: 99%

Nanotechnology for Animal Sciences-New Insights and Pitfalls: A Review

Kumar¹,

Singh²,

Chauhan³

et al. 2023

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Nanotechnology applications are increasingly recognized as potential tools in animal sciences, veterinary medicine, drug delivery, disease diagnosis and vaccine development. The nanoparticles and their therapeutic trends provide a realistic glimpse of current and future trends in veterinary diagnosis and medicine. Nanotechnology is ascribed to provide new horizons for applications in molecular biology and biotechnology by revolutionizing almost every field of veterinary or animal sciences by offering novel nano-scale tools and materials which can benefit society in all walks of life. The nanoparticles have been employed in diverse biological and non-biological functions. Nanomaterials can contribute to the farm animals for long-term sustainability by increasing the quality and quantity of safe, healthy and functional animal products. Although there are advantages while using nanoparticles to enhance performance and heal diseases in livestock, it is universally recognized that some nanoparticles can cause toxic and adverse effects on living creatures. Apart from that, the promise of nanoscience in veterinary medicine and livestock reproduction is yet to be explored. A few studies have been made on the uses of nanoparticles in veterinary medicine, livestock health and reproduction. Awareness is needed about the possible toxic effects and hazards, which could harm the living world. To improve the efficient and accurate diagnosis and treatment of animal diseases, more sophisticated research on nanomaterials and nanomedicines is required.

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Section: Assisted Reproductive Technologymentioning

confidence: 99%

Nanotechnology for Animal Sciences-New Insights and Pitfalls: A Review

Kumar¹,

Singh²,

Chauhan³

et al. 2023

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“…PI3K/Akt and ERK signaling pathways were implicated via an associated downregulation in transcript levels [ 294 ]. In another in vitro study utilizing a buffalo oocyte cell line, cells treated with selenium had a significantly faster rate of nuclear maturation, and decreased germinal vesicle stage [ 295 ]. While selenium treatment was not associated with differences in cell development at any other meiotic stage, several genes involved in oocyte gene expression were affected.…”

Section: Signals From Brain To Bodymentioning

confidence: 99%

“…While selenium treatment was not associated with differences in cell development at any other meiotic stage, several genes involved in oocyte gene expression were affected. Differential expression was noted in the downregulation of Casp3 and Amh , and the upregulation of Gpx4 and Sod [ 295 ]. Selenium treatment of buffalo oocytes was also associated with a reduction in Pla2g3 transcript levels [ 295 ], a phospholipase involved in lipid metabolism [ 296 ].…”

Section: Signals From Brain To Bodymentioning

confidence: 99%

“…Differential expression was noted in the downregulation of Casp3 and Amh , and the upregulation of Gpx4 and Sod [ 295 ]. Selenium treatment of buffalo oocytes was also associated with a reduction in Pla2g3 transcript levels [ 295 ], a phospholipase involved in lipid metabolism [ 296 ]. A study conducted on primary luteinized granulosa cells cultured from the ovarian follicles of goats found that selenium treatment stimulated cell proliferation and increased markers related to estradiol production (3β-HSD, p-Akt, cAMP, and steroidogenic acute regulatory protein) [ 297 ].…”

Section: Signals From Brain To Bodymentioning

confidence: 99%

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Selenium in Bodily Homeostasis: Hypothalamus, Hormones, and Highways of Communication

Toh

Nicholson

Vetter

et al. 2022

IJMS

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The ability of the body to maintain homeostasis requires constant communication between the brain and peripheral tissues. Different organs produce signals, often in the form of hormones, which are detected by the hypothalamus. In response, the hypothalamus alters its regulation of bodily processes, which is achieved through its own pathways of hormonal communication. The generation and transmission of the molecules involved in these bi-directional axes can be affected by redox balance. The essential trace element selenium is known to influence numerous physiological processes, including energy homeostasis, through its various redox functions. Selenium must be obtained through the diet and is used to synthesize selenoproteins, a family of proteins with mainly antioxidant functions. Alterations in selenium status have been correlated with homeostatic disturbances in humans and studies with animal models of selenoprotein dysfunction indicate a strong influence on energy balance. The relationship between selenium and energy metabolism is complicated, however, as selenium has been shown to participate in multiple levels of homeostatic communication. This review discusses the role of selenium in the various pathways of communication between the body and the brain that are essential for maintaining homeostasis.

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“…NPs containing various antioxidant materials can scavenge reactive oxygen species (ROS) and thus protect cellular molecules, such as lipids, proteins, and DNA, from oxidative stress, [ 11 , 12 , 13 ]. Therefore, many NPs are currently being used to improve the entire process of in vitro embryo production (IVP), such as gametes cryopreservation, oocyte in vitro maturation, and embryo culture [ 12 , 14 , 15 , 16 , 17 ], but some of these NPs exert toxic effects [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. To overcome these toxic effects, plant-derived nanoparticles are biologically safe and applicable for improving the quality of oocytes and subsequent embryo development [ 1 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

Roy

Qamar

Tanga

et al. 2021

Animals

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Molecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified Spirulina maxima pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus–oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT.

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Impact of nano-selenium on nuclear maturation and genes expression profile of buffalo oocytes matured in vitro

Cited by 7 publications

References 43 publications

Nanotechnology for Animal Sciences-New Insights and Pitfalls: A Review

Nanotechnology for Animal Sciences-New Insights and Pitfalls: A Review

Selenium in Bodily Homeostasis: Hypothalamus, Hormones, and Highways of Communication

Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

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