Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

Roy, Pantu Kumar; Qamar, Ahmad Yar; Tanga, Bereket Molla; Bang, Seonggyu; Seong, Gyeonghwan; Fang, Xun; Kim, Ghangyong; Edirisinghe, Shan Lakmal; Zoysa, Mahanama De; Kang, Do‐Hyung; Saadeldin, Islam M.; Cho, Jongki

doi:10.3390/ani11092483

Cited by 18 publications

(14 citation statements)

References 75 publications

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“…Real-time PCR was performed using a CFX Real-Time PCR System (Bio–Rad, Hercules, CA, USA). The PCR reaction protocol started with initial denaturation at 95 °C for 20 s, 50 °C for 20 s, and 72 °C for 20 s, respectively [ 41 , 42 , 43 ]. After the completion of PCR cycling, we acquired melting curves to verify the specificity and analyzed by 2 − △△Ct method [ 44 , 45 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Roy

Song

Park

2022

Foods

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Quercetin is an active nutraceutical element that is found in a variety of foods, vegetables, fruits, and other products. Due to its antioxidant properties, quercetin is a flexible functional food that has broad protective effects against a wide range of infectious and degenerative disorders. As a result, research is required on food-contact surfaces (rubber (R) and hand gloves (HG)) that can lead to cross-contamination. In this investigation, the inhibitory effects of quercetin, an antioxidant and antibacterial molecule, were investigated at sub-MIC (125; 1/2, 62.5; 1/4, and 31.25; 1/8 MIC, μg/mL) against Salmonella Typhimurium on surfaces. When quercetin (0–125 μg/mL) was observed on R and HG surfaces, the inhibitory effects were 0.09–2.49 and 0.20–2.43 log CFU/cm2, respectively (p < 0.05). The results were confirmed by field emission scanning electron microscopy (FE-SEM), because quercetin inhibited the biofilms by disturbing cell-to-cell connections and inducing cell lysis, resulting in the loss of normal cell morphology, and the motility (swimming and swarming) was significantly different at 1/4 and 1/2 MIC compared to the control. Quercetin significantly (p < 0.05) suppressed the expression levels of virulence and stress response (rpoS, avrA, and hilA) and quorum-sensing (luxS) genes. Our findings imply that plant-derived quercetin could be used as an antibiofilm agent in the food industry to prevent S. Typhimurium biofilm formation.

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Section: Methodsmentioning

confidence: 99%

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Roy

Song

Park

2022

Foods

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“…A CFX Real-Time PCR System was used to conduct the real-time PCR. Initial denaturation for the PCR reaction took place at 95, 50, and 72 °C for 20 s each [ 52 , 53 , 54 ]. The expression levels of the target genes were standardized and normalized in relation to the critical threshold (CT) mean values of rpIU as a housekeeping gene by the 2 − △△Ct method analysis [ 55 , 56 , 57 ].…”

Section: Methodsmentioning

confidence: 99%

The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence

2022

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Listeria monocytogenes is the species of foodborne pathogenic bacteria that causes the infection listeriosis. The food production chain employs various methods to control biofilms, although none are completely successful. This study evaluates the effectiveness of quercetin as a food additive in reducing L. monocytogenes mixed cultures (ATCC19113, ATCC19117, and ATCC15313) biofilm formation on stainless steel (SS), silicon rubber (SR), and hand glove (HG) coupons, as well as tests its antimicrobial activities. With a minimum inhibitory concentration (MIC) of 250 µg/mL, the tested quercetin exhibited the lowest bactericidal action with no visible bacterial growth. In contrast, during various experiments in this work, the inhibitory efficacy of quercetin at sub-MICs levels (1/2, 1/4, and 1/8 MIC) against L. monocytogenes was examined. A control group was not added with quercetin. The current study also investigates the effect of quercetin on the expression of different genes engaged in motility (flaA, fbp), QS (agrA), and virulence (hlyA, prfA). Through increasing quercetin concentration, swarming and swimming motility, biofilm formation, and expression levels of target genes linked to flagella motility, virulence, and quorum-sensing were all dramatically reduced. Quercetin (0–125 μg/mL) was investigated on the SS, SR, and HG surfaces; the inhibitory effects were 0.39–2.07, 0.09–1.96 and 0.03–1.69 log CFU/cm2, respectively (p < 0.05). Field-emission scanning electron microscopy (FE-SEM) corroborated the findings because quercetin prevented the development of biofilms by severing cell-to-cell contacts and inducing cell lysis, which resulted in the loss of normal cell shape. Our findings suggest that plant-derived quercetin should be used as an antimicrobial agent in the food industry to control the development of L. monocytogenes biofilms. These outcomes suggest that bacterial targets are of interest for biofilm reduction, with alternative natural food agents in the food sector along the entire food production chain.

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“…Similar to many other studies [ 33 – 35 ], this study showed that ROS was upregulated, and oxidative stress was induced in porcine oocytes as a result of rotenone treatment. In the previous study [ 36 – 38 ], GSH detected using Cell Tracker was the opposite concept of ROS, and the rotenone treated group decreased compared to the control group. As oxidative stress was speculated to be the result of rotenone-mediated suppression of mitochondrial function, mitochondrial function was also investigated.…”

Section: Discussionmentioning

confidence: 94%

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

Sun

Jiang

et al. 2022

PLoS ONE

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Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex Ⅰ. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. However, there is little precise information on the effects of rotenone exposure in porcine oocytes during in vitro maturation, and the mechanisms underlying these effects have not been determined. The Cumulus-oocyte complexes were supplemented with different concentrations of rotenone to elucidate the effects of rotenone exposure on the meiotic maturation of porcine oocytes during in vitro maturation for about 48 hours. First, we found that the maturation rate and expansion of cumulus cells were significantly reduced in the 3 and 5 μM rotenone-treated groups. Subsequently, the concentration of rotenone was determined to be 3 μM. Also, immunofluorescence, western blotting, and image quantification analyses were performed to test the rotenone exposure on the meiotic maturation, total and mitochondrial ROS, mitochondrial function and biogenesis, mitophagy and apoptosis in porcine oocytes. Further experiments showed that rotenone treatment induced mitochondrial dysfunction and failure of mitochondrial biogenesis by repressing the level of SIRT1 during in vitro maturation of porcine oocytes. In addition, rotenone treatment reduced the ratio of active mitochondria to total mitochondria, increased ROS production, and decreased ATP production. The levels of LC3 and active-caspase 3 were significantly increased by rotenone treatment, indicating that mitochondrial dysfunction induced by rotenone increased mitophagy but eventually led to apoptosis. Collectively, these results suggest that rotenone interferes with porcine oocyte maturation by inhibiting mitochondrial function.

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Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes

Cited by 18 publications

References 75 publications

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence

Rotenone causes mitochondrial dysfunction and prevents maturation in porcine oocytes

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