Impact of mosaic infection on growth and yield of sugarcane

Viswanathan, R.; Balamuralikrishnan, M.

doi:10.1007/bf02942419

Cited by 45 publications

(19 citation statements)

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“…Despite, mosaic is not a major problem in some countries; it has caused substantial yield losses in other countries by severe outbreak of the disease. Incidence of sugarcane mosaic is almost 100% in India and considering the vast area under sugarcane cultivation it results in significant yield losses [3][4][5]. Varietal degeneration due to mosaic is a serious concern that results in near extinction of elite cultivars from cultivation as witnessed recently to the erstwhile ruling varieties like Co 740 and CoC 671 in the tropical [5] and CoLk 8102, CoPant 90223, and CoS 767 in the subtropical [4] regions in India.…”

Section: Introductionmentioning

confidence: 98%

Identification of new variants of SCMV causing sugarcane mosaic in India and assessing their genetic diversity in relation to SCMV type strains

2009

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Sugarcane mosaic virus (SCMV) is one of the two causative viruses of mosaic in sugarcane, a sugar crop widely grown under tropical and subtropical conditions worldwide. Although molecular characterization of SCMV strains was reported from many countries, strains occurring in India, a major sugarcane producer have not been reported so far. Twenty-six sugarcane samples represented by seven major sugarcane growing states in India and USA were subjected to reverse-transcription polymerase chain reaction (RT-PCR) using a pair of newly designed coat protein specific primers. Among them 17 were found positive to the SCMV infection. The lengths of the sequences derived in this study using the new set of primers varied between 812 and 866 nt. The amino acid sequence comparison of 30 Indian SCMV isolates showed wide range of sequence similarities in core region (88.80-100%) and hyper variable region (51.3-100%). In the N-terminal region of the five Indian isolates, a deletion of 12 aa residues between aa 11 and 30 was observed, whereas the deletion was between aa 45 and 50 in SCMV-B and -D and between aa 61 and 70 in SCMV-A. The phylogenetic analyses performed with 46 SCMV CP sequences for both hyper variable region and core region separated the isolates mostly according to their geographical origin. The 30 Indian SCMV isolates were included exclusively in four groups besides SCMV-IND, which was grouped with SCMV-SC, a type of strain from Australia. Nearly 97.0% of the Indian isolates have no signs for close relationships with previously characterized SCMV type strains (SCMV-A, -B, -D, -E, and -SC) reported from other countries. Our studies revealed that the sugarcane mosaic in India are caused by at least nine new SCMV variants (IND-CC1, -CC2, -CC3, -CC4, -CO1, -CO2, -CP, -CS, and -J) and a type strain SCMV-SC represented by SCMV-IND. This is the first report on the variability and occurrence of new SCMV population in India.

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Section: Introductionmentioning

confidence: 98%

Identification of new variants of SCMV causing sugarcane mosaic in India and assessing their genetic diversity in relation to SCMV type strains

2009

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“…Looking the wide spread occurrence of these viruses infecting sugarcane crops, there is a need for immediate attention towards studying economic losses caused by mixed infection of either SCMV/SCSMV/SCYLV/SCBV in sugarcane. Already it was reported that mosaic and YLD cause significant impact on cane growth and juice quality parameters in many sugarcane varieties in India (Viswanathan, 2002;Viswanathan and Balamuralikrishnan, 2005).…”

Section: Discussionmentioning

confidence: 98%

“…Incidence of mosaic disease is almost 100% on sugarcane in the country and considering the vast area under sugarcane cultivation, it results in significant yield losses (Agnihotri,1996;Singh et al,2007;Viswanathan and Balamuralikrishnan, 2005). Only SCMV and Sorghum mosaic virus (SrMV) are two subgroup of potyviruses that cause mosaic on sugarcane under natural conditions and are considered as the causal agents of sugarcane mosaic (Grisham, 2000).…”

Section: Introductionmentioning

confidence: 97%

RT-PCR/PCR analysis detected mixed infection of DNA and RNA viruses infecting sugarcane crops in different states of India

et al. 2009

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“…Now it is clear that both SCMV and SCSMV are omnipresent in almost all the sugarcane growing countries of Asian Continent and possibly in other Continents also. Mosaic is the major cause of varietal degeneration in sugarcane, as it was witnessed from the degeneration of erstwhile ruling varieties like Co 740 and CoC 671 in tropical region (Viswanathan and Balamuralikrishnan, 2005) and CoLk 8102, CoPant 90223 and CoS 767 in subtropical region of the country. The use of virus free planting material is the best way to limit the effect of these viruses on varietal degeneration, as it is being clonally propagated.…”

Section: Introductionmentioning

confidence: 99%

Duplex — reverse transcription — polymerase chain reaction (D-RT-PCR)-a technique for the simultaneous detection of viruses causing sugarcane mosaic

2008

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Sugarcane mosaic virus (SCMV) and Sugarcane streak mosaic virus (SCSMV) are the two major RNA viruses that are widely prevalent in most of the Asian countries including India and are the causative viruses of mosaic in sugarcane. We have established that these two viruses either alone or in combination cause mosaic in sugarcane through RT-PCR. Further studies were conducted to detect the associated viruses in a single reaction. To optimize simultaneous detection of these viruses, a new set of primers were designed from the coat protein region of the viruses to suit duplex reverse transcription polymerase chain reaction (D-RT-PCR) and the conditions were standardized to amplify the target viruses in this assay. Of the 29 sugarcane samples tested for the simultaneous occurrence of SCMV and SCSMV, 10 sugarcane varieties were found to be infected with both the viruses with positive amplifications of ~860 bp and ~690 bp respectively, for SCMV and SCSMV. In the remaining 19 varieties, SCSMV was detected alone as the causative virus of mosaic. None of the selected varieties were found infected with SCMV alone. Further, the D-RT-PCR was found equally reliable to uniplex RT-PCR performed with SCMV and SCSMV primers in separate reactions. The D-RT-PCR products were cloned, sequenced and the respective sequence information confirmed the authenticity of the viruses amplified in D-RT-PCR. The lengths of the amplified fragments from SCMV isolates were ranged between 812 and 877 bp and in SCSMV it ranged from 683 to 689 bp. This diagnostic approach will be useful to establish distribution map of the virus(es) associated with mosaic in India and in other sugarcane growing countries.

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Impact of mosaic infection on growth and yield of sugarcane

Cited by 45 publications

References 4 publications

Identification of new variants of SCMV causing sugarcane mosaic in India and assessing their genetic diversity in relation to SCMV type strains

Identification of new variants of SCMV causing sugarcane mosaic in India and assessing their genetic diversity in relation to SCMV type strains

RT-PCR/PCR analysis detected mixed infection of DNA and RNA viruses infecting sugarcane crops in different states of India

Duplex — reverse transcription — polymerase chain reaction (D-RT-PCR)-a technique for the simultaneous detection of viruses causing sugarcane mosaic

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