2009
DOI: 10.1007/s12355-009-0064-y
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RT-PCR/PCR analysis detected mixed infection of DNA and RNA viruses infecting sugarcane crops in different states of India

Abstract: A sensitive assay based on the polymerase chain reaction detected mixed infection of four viruses, viz.

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Cited by 14 publications
(5 citation statements)
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“…Gonçalves et al (2002) optimized the AmpliDet (RNA) method for SCYLV detection in sugarcane and insect-vector (aphid: Melanaphis sacchari) and accuracy was analyzed with DAS-ELISA, RT-PCR and nucleic acid sequence-based amplification assay combined with northern blotting analysis techniques. In India, identification of SCYLV in both symptomatic and asymptomatic plants have been performed by RT-PCR using the virus specific primers (Singh et al, 2009;Viswanathan et al, 2008Viswanathan et al, , 2009Viswanathan et al, , 2010) ( Fig. 2A).…”
Section: Recognition Of Physical Properties Symptomatology and Diagmentioning
confidence: 99%
“…Gonçalves et al (2002) optimized the AmpliDet (RNA) method for SCYLV detection in sugarcane and insect-vector (aphid: Melanaphis sacchari) and accuracy was analyzed with DAS-ELISA, RT-PCR and nucleic acid sequence-based amplification assay combined with northern blotting analysis techniques. In India, identification of SCYLV in both symptomatic and asymptomatic plants have been performed by RT-PCR using the virus specific primers (Singh et al, 2009;Viswanathan et al, 2008Viswanathan et al, , 2009Viswanathan et al, , 2010) ( Fig. 2A).…”
Section: Recognition Of Physical Properties Symptomatology and Diagmentioning
confidence: 99%
“…Based on the PCR assay, Braithwaite et al [ 63 ] reported the occurrence of SCBV in noble canes ( Saccharum officinarum ), commercial hybrids, and clones within the ‘Saccharum complex’ including S. robustum , S. spontaneum , S. barberi , Erianthus arundinaceus , and E. ravennae . Singh et al [ 167 ] found mixed infection of SCBV with other viruses of sugarcane in India through PCR.…”
Section: Virus Characterizationmentioning
confidence: 99%
“…All the 102 samples of the nine different cultivars/varieties of sugarcane were observed with different intensity of mosaic symptoms. All the samples under investigation were found infected with SCMV using RT-PCR (Singh et al, 2009). On the other hand, Hema et al (1999) stated that SCMV subgroup viruses are not the cause of mosaic in sugarcane, it is due to SCSMV.…”
Section: Resultsmentioning
confidence: 99%