Impact of harvest product volume in erythrocyte depletion of allogeneic or autologous bone marrow using COBE spectra

Soydan, Ender; Ayyildiz, Erol; Dalva, Klara; Topçuoğlu, Pervin; Tol, Meltem; Arslan, Önder; İlhan, Osman; Arat, Mutlu

doi:10.1016/j.transci.2007.03.006

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…The recovery of MNCs was significantly lower than the CD34+ cell recovery using the Spectra Optia and is in line with previous studies using the COBE Spectra. The reason for the lower MNC recovery is not clear but it may be attributed in part to variation in specific gravity affecting the different position of CD34+ progenitor cells, lymphocytes, and monocytes in the buffy coat layer.…”

Section: Discussionsupporting

confidence: 91%

“…The study presented here demonstrates that the Spectra Optia can be used to effectively volume reduce and RBC deplete BM donations before transplantation. The mean volume of RBCs after processing was only 4.26 ± 1.62 mL, which is unlikely to cause a severe adverse reaction after infusion even when incompatible RBCs are present . In our study 62% of the transplants received donor‐recipient ABO‐incompatible grafts and none had adverse reactions at the time of infusion.…”

Section: Discussionmentioning

confidence: 53%

“…The mean volume of RBCs after processing was only 4.26 6 1.62 mL, which is unlikely to cause a severe adverse reaction after infusion even when incompatible RBCs are present. 5,14,15 In our study 62% of the transplants received donorrecipient ABO-incompatible grafts and none had adverse reactions at the time of infusion. The recovery of MNCs was significantly lower than the CD341 cell recovery using the Spectra Optia and is in line with previous studies 8,15 using the COBE Spectra.…”

Section: Discussionmentioning

confidence: 56%

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Human bone marrow processing using a new continuous‐flow cell separation device

et al. 2015

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 56%

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Human bone marrow processing using a new continuous‐flow cell separation device

et al. 2015

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“…Various authors have described concentration methods using blood cell separators, 12–17 which typically remove 78%–99% of the red blood cells, while their recovery rates are 78%–82% for mononucleated cells (MNCs) and 83%–100% for CD34+ cells. These results demonstrated that bone marrow cells can generally be collected reliably.…”

Section: Discussionmentioning

confidence: 99%

Concentration of Rat Bone Marrow Nucleated Cells Using Hypo-osmotic Hemolysis in Distilled Water

Ohnishi

Ochiai

Kishi

2014

Plastic and Reconstructive Surgery Global Open

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Background:Bone marrow aspirates contain primarily red blood cells. To achieve efficient cell transplantation for regeneration, the red blood cells need to be removed from the aspirates. Cell isolation is typically performed using density gradient centrifugation. However, this method entails issues of clinical safety and convenience. This study describes an efficient method to concentrate bone marrow nucleated cells by hypo-osmotic hemolysis.Methods:The optimal hemolysis conditions were determined by diluting the bone marrow suspensions with distilled water in various dilution ratios. Then, the resulting cell fractions were transplanted in a rat cranial defect model to evaluate their effects on bone formation and their angiogenic effects.Results:The optimal hemolysis conditions were a 3.3-fold dilution in distilled water and a hypo-osmotic exposure time of 45 seconds. Nucleated cells obtained using this method included granulocytes and mononuclear cells. These cells contain cytoplasmic angiogenic factors, including vascular endothelial growth factor, basic fibroblast growth factor, and hepatocyte growth factor. In a rat cranial defect model, callus formation and angiogenesis were significantly increased following transplantation of concentrated marrow nucleated cells in this manner.Conclusions:These results suggest angiogenic and osteogenetic effects of transplanting marrow nucleated cells using this hypo-osmotic method.

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“…To avoid adverse events, our common procedure for ABO incompatible stem cell transplantation includes red cell depletion of the BM graft. In the past few decades, erythrocyte depletion has been performed by automated procedures using commercially available cell separators [1–6]. For routine procedures, we had used the Fenwal CS 3000 omnix™ plus (Fenwal, Lake Zurich, IL) in our department.…”

Section: Introductionmentioning

confidence: 99%