Bone marrow processing with the AMICUS™ separator system

Abstract

We conclude that, in principle, the Amicus device can be used for MNC collection from BM to deplete erythrocytes from BM grafts in allogeneic stem cell transplantations.

Red blood cell depletion from bone marrow and peripheral blood buffy coat: a comparison of two new and three established technologies

“…Since RBCs, white blood cells (WBCs), platelets (PLTs), and plasma differ in size and density, they can be efficiently separated by density gradient centrifugation or apheresis, and both have been applied successfully. [6][7][8][9] The critical variables for the success of RBC depletion of BM are recovery of CD341 "stem cells," which are contained within the mononuclear cell (MNC) population and, when performed in the context of ABO majorincompatible BMT, near-qualitative removal of RBCs to values below 0.5 mL/kg body weight of the recipient. Similar processing of whole blood, buffy coats (BCs), and occasionally, apheresis products generally also aims for best-possible RBC depletion while maximally recovering MNCs.…”

Bone marrow processing for hematopoietic stem cell transplantation: recommendations of the SFGM-TC

“…or automatic devices (Terumo/Cobe Spectra Optia Apheresis System, etc.) ( [3], [4], [5], [6], unpublished data from Boulanger).…”

A comparison of two protocols for optimal red blood cell depletion using Sepax-2 device for ABO-major incompatible transplantation in adults

“…Various methods have been used for RBC depletion such as RBC sedimentation using Hydroxyethyl starch (HES) sedimentation or density gradientbased separation (DGBS) over Ficoll-Paque using COBE 2991 (Terumo BCT) [3] or not [4,5]. Studies have demonstrated feasibility and clinically efficiencies for centrifugation using automated or semi-automated separator devices such as Haemonetics Model 30 [6], Amicus (Fresenius-Kabi) and Fenwal CS3000 Omnix plus devices [7] or COBE Spectra [3,8,9], albeit with some quantitative variations between methods for RBC depletion and hematopoietic progenitor cells (HPC) recovery. The newly available BMP kit (Bone Marrow Program) available with the Spectra Optia device achieved results in a similar range as those reported for Optia's predecessor technology, the COBE Spectra [10][11][12][13].…”