Red blood cell depletion from bone marrow and peripheral blood buffy coat: a comparison of two new and three established technologies

Abstract

All methods are in principle suitable and can be selected based on sample volume, available technology, and desired product specifications beyond RBC depletion and MNC and/or CD34+ cell recovery.

Robust Production of Cytomegalovirus pp65-Specific T Cells Using a Fully Automated IFN-γ Cytokine Capture System

“…To overcome these limitations, the CliniMACS Prodigy was developed as a technical upgrade over the CliniMACS Plus, allowing a fully automated system. The Clini-MACS Prodigy (Miltenyi Biotec) was initially described for its automated density gradient separation, including red blood cell depletion, magnetic cell separation, and cell culture [17]. Recently, the integration of IFN-γ CCS software into the CliniMACS Prodigy has proven to be feasible for the automated production of CMV-specific T cells [18].…”

Ficoll‐hypaque separation vs whole blood lysis: Comparison of efficiency and impact on minimal residual disease analysis

“…9 Moreover, the lysis solution has a permeabilizing effect on total WBCs, making this approach more suitable for intracellular antigen detection. In transfusional and flow cytometry daily practice, this procedure is used for preparation of ABO-incompatible BM transplantations and for leukocyte cell surface antigen analysis.…”

“…In transfusional and flow cytometry daily practice, this procedure is used for preparation of ABO-incompatible BM transplantations and for leukocyte cell surface antigen analysis. 9 Moreover, the lysis solution has a permeabilizing effect on total WBCs, making this approach more suitable for intracellular antigen detection. 10 On the other side, Ficoll separation provides a more purified cell population, ideal for functional studies.…”

Infusion hemolysis after pediatric major ABO‐mismatched bone marrow transplant: Comparison of two red blood cell depletion techniques

“…18 The alternative approach, graft manipulation to decrease the amount of incompatible RBCs, can be accomplished with various different RBC depletion techniques including hydroxyethyl starch (HES) sedimentation, Ficoll density gradient separation, and blood cell separator processing. [19][20][21][22][23] Evidence-based guidelines regarding the prevention of major ABOmismatched BMT infusion hemolysis do not exist. Regarding RBC depletion in particular, transplant centers employ different methods and have varied standards with regard to the amount of residual incompatible RBCs that can be infused with the bone marrow graft.…”