Immunostimulant activities of the aqueous methanolic extract of Leptadenia pyrotechnica , a plant from Cholistan desert

Rasheed, Hafiz Muhammad Farhan; Rasheed, Fahad; Qureshi, Abdul Wahid; Jabeen, Qaiser

doi:10.1016/j.jep.2016.03.039

Cited by 31 publications

(30 citation statements)

References 38 publications

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“…They have consisted of extracts of a single medicinal herb [15–17], an herbal decoction or extract combination [4–6, 18–21], or active fractions or single components isolated from medicinal herbs [1, 22–25]. Their protective effects have been investigated using experimental animal models [1, 4, 5, 15–18, 20–25] or clinical human studies enrolling cancer patients [6, 19]. However, to the best of our knowledge, this is the first report on using a traditional folk medicine to reverse docetaxel-induced myelotoxicity and on the myelo-protective effect of Phragmitis rhizoma .…”

Section: Discussionmentioning

confidence: 99%

Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo

Kim¹,

Lee²,

Kim³

et al. 2017

BMC Complement Altern Med

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BackgroundA variety of anticancer chemotherapeutics induce adverse side effects including myelotoxicity. Dried roots of Phragmites communis Trinius, Phragmitis rhizoma, have been clinically used in traditional folk medicine to relieve various symptoms like fever. In this study, we evaluated the protective effect of the aqueous extract of Phragmitis rhizoma (EPR) against docetaxel-induced myelotoxicity in vitro and in vivo.MethodsThe in vitro myelo-protective effect of EPR was evaluated using the colony forming unit (CFU) assay with hematopoietic progenitor cells. The in vivo efficacy of EPR was evaluated in myelosuppressed C57BL/6 male mice which were induced by repeated intraperitoneal injections of 30 mg/kg docetaxel for 3 times. EPR was orally administered for 4 days to docetaxel-induced myelosuppressed C57BL/6 male mice which were induced by intraperitoneal injection of 30 mg/kg docetaxel for 3 times: Group 1 (vehicle control, n = 10), Group 2 (docetaxel plus vehicle, n = 10), Group 3 (docetaxel plus EPR 30 mg/kg, n = 10), Group 4 (docetaxel plus EPR 100 mg/kg, n = 10) and Group 5 (docetaxel plus EPR 300 mg/kg, n = 10). Whole blood counts were measured automatically, and immune organs were histologically examined. Expression of immunomodulatory cytokines was measured by quantitative real-time polymerase chain reaction or enzyme-linked immunosorbent assay. The toxicity of EPR itself was evaluated in normal human cell lines including IMR-90, foreskin fibroblast and human umbilical vein endothelial cells. The hepatotoxicity of EPR was predicted by multi-parametric assays involving cell viability, caspase 3/7 activity, GSH contents and LDH leakage using the HepaRG hepatic cell line.ResultsCo-treatment of EPR or its major component, p-hydroxycinnamic acid, increased the numbers of hematopoietic CFU counts in the docetaxel-induced in vitro myelotoxicity assay system. The in vitro protective effect of EPR against docetaxel toxicity was replicated in a myelosuppressed animal model: white blood cells, neutrophils, lymphocytes and red blood cells rebounded; bone marrow niche and structural integrity of the thymus were preserved; and the expression of immune-stimulating cytokines including IL3, IL6, SCF and GM-CSF was enhanced. Furthermore, EPR and p-hydroxycinnamic acid promoted the proliferation of primary splenocytes and thymocytes. In the toxicity assays, no remarkable signs related with toxicity were observed in all tested normal human cells and HepaRG.ConclusionsEPR has the potential to ameliorate docetaxel-mediated myelotoxicity in both in vitro and in vivo models. However, the identification of the responsible active components and the precise underlying myelo-protective mechanism of EPR need to be elucidated before novel drug development using EPR can precede.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1890-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo

Kim¹,

Lee²,

Kim³

et al. 2017

BMC Complement Altern Med

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“…1000, 3000, 5000 and 10000 mg/kg p.o. The animals were observed for changes in behavioral and physiological parameters including grooming, hyperactivity, convulsions, alertness, lacrimation, urination, corneal reflex, writhing reflex, righting reflex and gripping strength for 12 hours, on an hourly basis, and for the mortality for 48 hours and then for 14 days, on daily basis (Rasheed et al, 2016).…”

Section: Acute Toxicity Studiesmentioning

confidence: 99%

Effect of Abutilon indicum in thyroxine-induced hyperthyroidism in rat

Jabeen

Khan

Qureshi

et al. 2021

Bangladesh J Pharmacol

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The study was designed to investigate the antithyroid activity of the crude methanolic (70%) extract of aerial parts of Abutilon indicum in male albino rats. The extract was prepared and analyzed for the presence of phytochemical constituents through preliminary chemical analysis, antioxidant assay and GC-MS. The in vivo antithyroid activities in thyroxine-induced hyperthyroidism were studied. Phytochemical analysis showed the presence of alkaloids, flavonoids and phenols, also verified by the data obtained from GC-MS. Thyroxine increased the levels of triiodothyronine (4.9 ± 0.1 ng/mL) and total thyroxine (9.4 ± 0.2 μg/dL); while, A. indicum at the doses of 300 and 500 mg/kg, showed significant decrease in the elevated levels of triiodothyronine (3.0 ± 0.1 and 2.6 ± 0.2 ng/mL) and thyroxine (7.7 ± 0.2 and 7.1 ± 0.2 μg/dL), respectively. Histopathological studies showed the restoration of filled follicular colloids in extract-treated animals. The results show that A. indicum exhibits dose-dependent antithyroid effects.

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“…The immunostimulants, including medicines, chemicals, and natural products might improve immune responses through non-specific as well as specific defense mechanisms [1]. The major side effects caused by the frequent use of chemotherapeutic agents for immunomodulation include cytotoxicity to normal cells and immunosuppressive actions [2]. Cyclophosphamide (CTX) is one of the most widely used anticancer agent and a potent immunosuppression drug that can inhibit both humoral and cell mediated immunity [3,4].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Potential of the Industrialized Houttuynia cordata Fermentation Product In Vitro and in Wistar Rats

Utaiwat

Senawong

Khongsukwiwat

et al. 2021

Foods

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Houttuynia cordata fermentation products (HCFPs) are produced and widely used as dietary supplements for health and immune support. However, the effect on immune function for these products has not been clearly demonstrated. In this study, soluble fractions of the selected HCFP were used for determination of the immunomodulatory potential, both in vitro and in animal models. Viability and proliferation of rat splenocytes and phagocytic activity of human neutrophils were evaluated. Studies on immunomodulatory effects, including hematological parameters, mitogen-driven lymphocyte proliferation and hemagglutination, were performed in both healthy and immunosuppressed rats. Soluble fraction of the selected HCFP significantly enhanced phagocytic activity of human neutrophils and tended to stimulate splenocyte viability and proliferation. There was no morbidity or mortality for administration of a 14-day regimen of the selected HCFP in both male and female rats. The healthy rats treated with HCFP gained body weight less than the control group, suggesting a reduction in calorie intake. Moreover, low dose of HCFP caused an increased B cell proliferation in ex-vivo, which was related to the increased antibody titer against SRBC in immunosuppressed rats. Our results indicate that the selected HCFP enhances the phagocytic activity of the neutrophils and augments the antibody production in immunosuppressed rats.

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Immunostimulant activities of the aqueous methanolic extract of Leptadenia pyrotechnica , a plant from Cholistan desert

Cited by 31 publications

References 38 publications

Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo

Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo

Effect of Abutilon indicum in thyroxine-induced hyperthyroidism in rat

Immunomodulatory Potential of the Industrialized Houttuynia cordata Fermentation Product In Vitro and in Wistar Rats

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