13C discrimination between atmosphere and bulk leaf matter (D 13 Clb) is frequently used as a proxy for transpiration efficiency (TE). Nevertheless, its relevance is challenged due to: (1) potential deviations from the theoretical discrimination model, and (2) complex time integration and upscaling from leaf to whole plant. Six hybrid genotypes of Populus deltoides ¥ nigra genotypes were grown in climate chambers and tested for whole-plant TE (i.e. accumulated biomass/water transpired). Net CO2 assimilation rates (A) and stomatal conductance (gs) were recorded in parallel to: (1) 13 C in leaf bulk material (d 13 Clb) and in soluble sugars (d 13 Css) and (2) 18 O in leaf water and bulk leaf material. Genotypic means of d 13 Clb and d 13 Css were tightly correlated. Discrimination between atmosphere and soluble sugars was correlated with daily intrinsic TE at leaf level (daily mean A/gs), and with whole-plant TE. Finally, gs was positively correlated to 18 O enrichment of bulk matter or water of leaves at individual level, but not at genotype level. We conclude that D 13 Clb captures efficiently the genetic variability of whole-plant TE in poplar. Nevertheless, scaling from leaf level to whole-plant TE requires to take into account water losses and respiration independent of photosynthesis, which remain poorly documented.

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Poplar genotypes differ in transpiration efficiency (TE) at leaf and whole-plant level under similar conditions. We tested whether atmospheric vapour pressure deficit (VPD) affected TE to the same extent across genotypes. Six Populus nigra genotypes were grown under two VPD. We recorded (1) (13)C content in soluble sugars; (2) (18)O enrichment in leaf water; (3) leaf-level gas exchange; and (4) whole-plant biomass accumulation and water use. Whole-plant and intrinsic leaf TE and (13)C content in soluble sugars differed significantly among genotypes. Stomatal conductance contributed more to these differences than net CO2 assimilation rate. VPD increased water use and reduced whole-plant TE. It increased intrinsic leaf-level TE due to a decline in stomatal conductance. It also promoted higher (18)O enrichment in leaf water. VPD had no genotype-specific effect. We detected a deviation in the relationship between (13)C in leaf sugars and (13)C predicted from gas exchange and the standard discrimination model. This may be partly due to genotypic differences in mesophyll conductance, and to its lack of sensitivity to VPD. Leaf-level (13)C discrimination was a powerful predictor of the genetic variability of whole-plant TE irrespective of VPD during growth.

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Genetic differences in δ¹³C (isotopic composition of dry matter carbon) have been evidenced among poplar genotypes at juvenile stages. To check whether such differences were maintained with age in trees growing in plantations, we investigated the time course of δ¹³C as recorded in annual tree rings from different genotypes growing at three sites in southwestern France and felled at ∼15-17 years. Wood cores were cut from tree discs to record the time course of annual basal area increment (BAI). The isotopic ratio δ¹³C was recorded in bulk wood and in extracted cellulose from the annual rings corresponding to the period 1996-2005. Discrimination against ¹³C between atmosphere and tissues (Δ¹³C) was computed by taking into account the inter-annual time course of δ¹³C in the atmosphere. Annual BAI increased steadily and stabilized at about 8 years. An offset in δ¹³C of ∼1‰ was recorded between extracted cellulose and bulk wood. It was relatively stable among genotypes within sites but varied among sites and increased slightly with age. Site effects as well as genotype differences were detected in Δ¹³C recorded from the cellulose fraction. Absolute values as well as the genotype ranking of Δ¹³C remained stable with age in the three sites. Genotype means of Δ¹³C were not correlated to annual BAI. We conclude that genotypic differences of Δ¹³C occur in older poplar trees in plantations, and that the differences as well as the genotype ranking remain stable while trees age until harvest.

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A greenhouse experiment was conducted to investigate the effects of water deficit on growth and physiological parameters of Ficus benjamina and Conocarpus erectus. The results revealed that all growth parameters such as plant height, stem diameter, no. of leaves, no. of branches and chlorophyll contents significantly decreased under water deficit condition. Interestingly, although leaf, stem and total biomass production and allocation decreased significantly under water deficit, but root biomass production and allocation increased significantly. Similarly, stomatal conductance to water vapor decreased significantly and CO2 assimilation rate remained similar to control under water deficit condition. Resultantly, a significant increase in water use efficiency was evident in both species under water deficit condition. These results suggested that, in spite of a significant decrease in biomass production, young Conocarpus erectus and Ficus benjamina can tolerate water deficit which is due to sustained CO2 assimilation rate and increase in root biomass.

Low water availability predicted under climate change is a major abiotic factor limiting plants growth and productivity. In this study a greenhouse experiment was conducted on three important tree species of arid environment: Conocarpus erectus (CE), Acacia modesta (AM), and Salix tetrasperma (ST). Young saplings were subjected to control (C), medium (MWD) and severe soil water deficit (SWD) treatments and response was evaluated. Results showed that in all the three species leaf, stem and root dry weight production remained similar to C under MWD treatment but decreased significantly under SWD. The highest decrease in total dry weight was noticed in ST and the lowest was evidenced in AM under SWD. Root:shoot ratio increased significantly in both CE and AM under MWD and SWD. Furthermore, chlorophyll content decreased while proline content increased significantly in both MWD and SWD treatments. The production of oxidants (hydrogen peroxide and superoxide anions) and antioxidants (superoxide dismutase, catalase, peroxidase and ascorbate peroxidase) increased significantly under both MWD and SWD treatments and were the highest in AM in both MWD and SWD treatments. Therefore, we may conclude that all the three species can tolerate medium water stress due to increased root production and an effective antioxidant defense mechanism.