Immunoprecipitation of bisphenol A by antibody–mesoporous silica composites

Abstract

Bisphenol A (BPA) is of global concern because of its disruption of endocrine systems and ubiquity in aquatic environment. In this study, BPA antibody was successfully immobilised on novel mesoporous silica (MPS) carriers that display unique properties such as high surface area, highly uniform pore distribution and high adsorption capacity. Mobil Crystalline Material MCM-41 (2.7 nm), Santa Barbara Amorphous SBA-15-1 (12.3 nm) and SBA-15-2 (24.0 nm) materials were used as supports for these antibodies. On these carriers, the BPA antibody immobilisation reached 40 g mg −1. For each MPS, 15 ng of BPA antigen was adsorbed on 1 mg of MPS-antibody composite, which resulted in an antibody activity of 30%. The highest recovery rate of BPA antigen was observed for 80% acetonitrile in 10 mM phosphate buffer (pH 7). After six repeated runs, BPA antibodies immobilised on SBA-15-1 and SBA-15-2 retained about 30% of their initial activity. In contrast, these antibodies showed 13% lower residual activity on MCM-41 than on SBA-15-1 and SBA-15-2. This result indicated that entire antibody molecules were adsorbed inside SBA-15-1 and SBA-15-2 pores, stabilising their structural conformation.

Molecular Layer Deposition for Applications in Lithium-Ion Batteries

“…introduction From a technological point of view, porous thin films are of interest due to the variety of possible application areas. These include among other: separators [86], filtration membranes [87][88][89], catalysis [90][91][92][93][94][95], sensors [96,97], protective coatings [98] and medicine [99][100][101]. Sol-gel [102], hydrothermal [103], and anodic oxidation methods [94,95,104] exist to synthesize inorganic porous films.…”

Gold nanoparticle–mesoporous silica sheet composites with enhanced antibody adsorption capacity

“…Antibodies have been immobilised on a wide variety of solid materials for practical applications, including immunoassays, nanomedicine, detection and separation. [1][2][3][4] Nano-shells, polymers, porous materials, agarose and gold nanorods have all been used as IgG adsorbents. [5][6][7] For the immobilisation of IgG on an adsorbent, it is known that chemical binding is useful, particularly in Fab region-specific binding (an antigen-antibody complex reaction).…”