Immunomodulatory Effect of Propolis Extract on Granzyme Expression in CD8+ and CD4+CD25+ T Cells

Kusnul, Zauhani; Rahayu, Pudji; Rifa’i, Muhaimin; Widjajanto, Edi

doi:10.25002/tji.2017.544

Cited by 4 publications

(6 citation statements)

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“…Granzyme is a cytolytic enzyme produced cytotoxic T cells that play a role in lysing target cells, including cancer cells. Results of Kusnul et al [72] showed that the increased level of CD8 + T cells-expressing granzyme and a decreased level in CD4 + CD25 + T cells-expressing granzyme by propolis extract were both provide positive benefits in cancer therapy.…”

Section: Immunomodulatory Effects On the Function Of T And B Lymphocymentioning

confidence: 99%

“…Scheller et al [79] observed the cytotoxic effect of ethanol extract of propolis in micebearing Ehrlich carcinoma in vivo. The NK activity of non-adherent spleen cells was evaluated by the 51 Crrelease cytotoxicity assay against murine lymphoma (Yac-1) target cells [72] . Furthermore, an inhibitory effect of aqueous and ethanol extracts of propolis on colon carcinogenesis was reported in rats [80,81] .…”

Section: Immunomodulatory Effects On the Function Of Natural Killer (mentioning

confidence: 99%

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Immunomodulatory Effects of Propolis and its Components on Basic Immune Cell Functions

Wolska¹,

Górska²,

Antosik³

et al. 2019

pharmaceutical-sciences

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Propolis is a product of the vital activity of the honey bee Apis mellifera. Bees produce propolis by mixing substances gathered from budding plants, flower buds and resinous exudates. They thus produce a material suitable for closing gaps, embalming dead insects within the beehive and protection from invasion by microorganisms and insects. This activity of propolis results from its composition. Raw propolis is typically composed of 50 % plant resins, 30 % waxes, 10 % essential and aromatic oils, 5 % pollens and 5 % other organic substances [1]. Propolis possessed a variety of biological and pharmacological effects, such as antibacterial, antioxidant, antitumour, antiinflammatory and immunomodulatory. However, propolis could not be used as a raw material, so it must be purified by extraction to remove the inert material and preserve the polyphenolic fraction, which is primarily responsible for its activity [1,2]. Generally, ethanol is the best solvent for propolis preparation, but other solvents such as ethyl ether, water, methanol and chloroform could also be used for extraction and identification of propolis constituents [3]. More than 300 different compounds have been identified in propolis, including phenols, tannins, polysaccharides, terpenes, aliphatic acids, esters, aromatic acids, fatty acids, aldehydes, amino

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Section: Immunomodulatory Effects On the Function Of T And B Lymphocymentioning

confidence: 99%

Section: Immunomodulatory Effects On the Function Of Natural Killer (mentioning

confidence: 99%

Immunomodulatory Effects of Propolis and its Components on Basic Immune Cell Functions

Wolska¹,

Górska²,

Antosik³

et al. 2019

pharmaceutical-sciences

View full text Add to dashboard Cite

show abstract

“…Moreover, Rifa'I and Widodo (2014) reported that propolis administration in the mouse changes CD4 and CD8 immune responses. Compared to the control group, the CD4+, and CD8+ T-lymphocyte levels are not significantly reduced in propolis therapy at all dosages in cell culture ( Kusnul et al, 2017 ). Moreover, Draganova-Filipova et al (2010) found that low doses of propolis unaffected CD4+ and CD8+ T-lymphocyte percentages.…”

Section: Discussionmentioning

confidence: 98%

Effects of ginger extract and/or propolis extract on immune system parameters of vaccinated broilers

et al. 2023

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“…5 Propolis merupakan produk lebah yang memiliki berbagai efek terapi, seperti anti bakteri, 6 anti tumor, 7 dan imunomodulator. 8 Pada penelitian sebelumnya telah dilakukan studi pendahuluan terhadap kandungan senyawa aktif propolis dan didapatkan data bahwa ekstrak propolis mengandung senyawa aktif penting seperti; caffeic acid phenethyl ester (CAPE), artepillin C, quercetin dan hesperidin yang merupakan senyawa-senyawa berkhasiat dari propolis. 9 Ekstrak propolis juga dibuktikan memiliki aktifitas sebagai immunomodulator dengan meningkatkan ekspresi granzyme oleh sel T sitotoksik in vitro.…”

Section: Pendahuluanunclassified

“…9 Ekstrak propolis juga dibuktikan memiliki aktifitas sebagai immunomodulator dengan meningkatkan ekspresi granzyme oleh sel T sitotoksik in vitro. 8 Secara in vivo ekstrak propolis juga telah dibuktikan mampu meningkatkan respon imun anti kanker pada tikus Spraque-Dawley yang diinduksi mengalami kanker payudara dengan menggunakan DMBA. 10 Selain itu keamanan penggunaan ekstrak propolis terhadap organ ginjal dan hepar juga telah diuji dengan uji toksisitas subkronik pada tikus wistar dan hasilnya menunjukkan bahwa ekstrak propolis aman untuk dikonsumsi (data belum dipublikasi).…”

Section: Pendahuluanunclassified

Ekstrak Propolis Memperbaiki Profil Berat Badan Tikus Model Kanker Payudara yang Diinduksi dengan 7,12-dymethyilbenz(a) antracene (DMBA)

Kusnul¹,

Suryono²,

Tamsuri³

2019

mpk

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Body weight is a general indicator for assessing health status. Various diseases cause drastic weight loss, including cancer. Propolis is a bee product that has various therapeutic effects such as; anti-bacterial, antitumor, antioxidant and immunomodulatory. Propolis is also reported to be able to reduce digestive organ disorders, increase appetite and improve metabolic processes. Chemicals such as 7.12-dymethyilbenz (a) antracene (DMBA) are widely reported to have strong carcinogenic effects, especially in Sprague–Dawley rat. This study aims to assess the effect of propolis extract on the weight of Sprague–Dawley female rat induced with DMBA (7,12-dymethylbenz (a) antracene). Twenty-four female Sprague–Dawley rats 45-50 days old were induced by DMBA with a combination of injection and oral methods, as negative controls 6 Sprague–Dawley rats without DMBA induction. At the 11th week randomized negative control rats and DMBA treated rats were taken for histopathological examination of breast tissue. After it was found that rat with DMBA treatment were positive for breast cancer, in the 12th week the rat that had received DMBA treatment were divided into 4 groups, 3 groups received oral propolis extract through a gastric sonde with doses 50, 100 and 200 mg in 1 ml of corn oil, 1 group as a positive control did not get the treatment of propolis extract. Body weight is weighed before starting treatment and monitored every two weeks to 15 weeks. The results of weighing showed that the group of rat that received DMBA increased their body weight lower than the group without DMBA, and then the treatment group of propolis extract increased their body weight higher than the group without the treatment of propolis extract. The results showed that the treatment of propolis extract had a potency to improve the body weight profile of rat breast cancer model induced by DMBA. Abstrak Berat badan merupakan indikator umum untuk menilai status kesehatan. Berbagai penyakit menyebabkan penurunan berat badan yang drastis, diantaranya adalah kanker. Propolis merupakan produk lebah yang memiliki berbagai efek terapi seperti; anti bakteri, anti tumor, antioksidan dan imunomodulator. Propolis juga dilaporkan mampu menurunkan gangguan organ pencernaan, peningkatan nafsu makan, dan perbaikan proses metabolisme. Bahan kimia seperti 7,12-dymethyilbenz(a)antracene (DMBA) banyak dilaporkan memiliki efek karsinogenik yang kuat khususnya terhadap tikus Sprague–Dawley. Penelitian ini bertujuan untuk menilai pengaruh pemberian ekstrak propolis terhadap berat badan tikus Sprague–Dawley betina yang diinduksi untuk mengalami kanker payudara dengan DMBA. Sebanyak 24 ekor tikus Sprague– Dawley betina berumur 45-50 hari diinduksi dengan DMBA dengan kombinasi metode injeksi dan oral, sebagai kontrol negatif 6 ekor tikus Sprague–Dawley tanpa induksi DMBA. Pada minggu ke-11 diambil secara acak tikus kontrol negatif dan tikus perlakuan DMBA untuk dilakukan pemeriksaan histolopatogi jaringan payudara. Setelah didapatkan bahwa tikus dengan perlakuan DMBA positif mengalami kanker payudara, pada minggu ke-12 tikus yang telah mendapat perlakuan DMBA dibagi menjadi 4 kelompok, 3 kelompok mendapat ekstrak propolis oral melalui sonde dengan dosis masing-masing 50, 100, dan 200 mg dalam 1 ml minyak jagung, 1 kelompok sebagai kontrol positif tidak mendapat perlakuan ekstrak propolis. Berat badan ditimbang sebelum mulai perlakuan dan dipantau tiap dua minggu sampai 15 minggu. Hasil penimbangan berat badan menunjukkan bahwa kelompok tikus yang mendapat DMBA peningkatan berat badannya lebih rendah dibanding kelompok tanpa DMBA, dan selanjutnya kelompok perlakuan ekstrak propolis kenaikan berat badannya lebih tinggi dibanding kelompok tanpa perlakuan ekstrak propolis. Hasil penelitian menunjukkan bahwa perlakuan ekstrak propolis memiliki potensi memperbaiki profil berat badan tikus model kanker payudara yang diinduksi dengan DMBA.

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Immunomodulatory Effect of Propolis Extract on Granzyme Expression in CD8+ and CD4+CD25+ T Cells

Cited by 4 publications

References 0 publications

Immunomodulatory Effects of Propolis and its Components on Basic Immune Cell Functions

Immunomodulatory Effects of Propolis and its Components on Basic Immune Cell Functions

Effects of ginger extract and/or propolis extract on immune system parameters of vaccinated broilers

Ekstrak Propolis Memperbaiki Profil Berat Badan Tikus Model Kanker Payudara yang Diinduksi dengan 7,12-dymethyilbenz(a) antracene (DMBA)

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