Propolis is a product of the vital activity of the honey bee Apis mellifera. Bees produce propolis by mixing substances gathered from budding plants, flower buds and resinous exudates. They thus produce a material suitable for closing gaps, embalming dead insects within the beehive and protection from invasion by microorganisms and insects. This activity of propolis results from its composition. Raw propolis is typically composed of 50 % plant resins, 30 % waxes, 10 % essential and aromatic oils, 5 % pollens and 5 % other organic substances [1]. Propolis possessed a variety of biological and pharmacological effects, such as antibacterial, antioxidant, antitumour, antiinflammatory and immunomodulatory. However, propolis could not be used as a raw material, so it must be purified by extraction to remove the inert material and preserve the polyphenolic fraction, which is primarily responsible for its activity [1,2]. Generally, ethanol is the best solvent for propolis preparation, but other solvents such as ethyl ether, water, methanol and chloroform could also be used for extraction and identification of propolis constituents [3]. More than 300 different compounds have been identified in propolis, including phenols, tannins, polysaccharides, terpenes, aliphatic acids, esters, aromatic acids, fatty acids, aldehydes, amino
