Immunologic patterns associated with cure in human American cutaneous leishmaniasis

Coutinho, S.; Da‐Cruz, Alda Maria; Bertho, Álvaro Luiz; Santiago, Marta A; de-Luca, Paula Melo

doi:10.1590/s0100-879x1998000100019

Cited by 46 publications

(44 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the small number of patients studied, we can speculate that the low percentage of apoptosis in CD8 + T cells of spontaneous healing patients may point to a significant role of this cell subpopulation in the mechanism of cure of LCL. This possibility is in accordance with previous results from our group (36)(37)(38)(39) showing that CD8 + T cells are associated with cure and protection in LCL.…”

Section: Discussionsupporting

confidence: 93%

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis

Bertho

Santiago

Da‐Cruz

et al. 2000

Braz J Med Biol Res

Self Cite

View full text Add to dashboard Cite

Human localized cutaneous leishmaniasis (LCL), induced by Leishmania braziliensis, ranges from a clinically mild, self-healing disease with localized cutaneous lesions to severe forms which can present secondary metastatic lesions. The T cell-mediated immune response is extremely important to define the outcome of the disease; however, the underlying mechanisms involved are not fully understood. A flow cytometric analysis of incorporation of 7-amino actinomycin D and CD4 + or CD8 + T cell surface phenotyping was used to determine whether different frequencies of early apoptosis or accidental cell death occur at different stages of LCL lesions. When all cells obtained from a biopsy sample were analyzed, larger numbers of early apoptotic and dead cells were observed in lesions from patients with active disease (mean = 39.5 ± 2.7%) as compared with lesions undergoing spontaneous healing (mean = 17.8 ± 2.2%). Cells displaying normal viability patterns obtained from active LCL lesions showed higher numbers of early apoptotic events among CD8 + than among CD4 + T cells (mean = 28.5 ± 3.8 and 15.3 ± 3.0%, respectively). The higher frequency of cell death events in CD8 + T cells from patients with LCL may be associated with an active form of the disease. In addition, low frequencies of early apoptotic events among the CD8 + T cells were observed in two patients with self-healing lesions. Although the number of patients in the latter group was small, it is possible to speculate that, during the immune response, differences in apoptotic events in CD4 + and CD8 + T cell subsets could be responsible for controlling the CD4/CD8 ratio, thus leading to healing or maintenance of disease. Correspondence

show abstract

Section: Discussionsupporting

confidence: 93%

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis

Bertho

Santiago

Da‐Cruz

et al. 2000

Braz J Med Biol Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our study focused primarily on the production of IFN-γ by these cells due to the involvement of this cytokine in resistance to and recovery from leishmanial infections (Carvalho et al 1995, Coutinho et al 1998, Ribeirode-Jesus et al 1998. When analyzing the production of this cytokine before treatment onset it was observed that cells from patients with chronic lesions produced more IFN-γ than those from patients with recent infections.…”

Section: Discussionmentioning

confidence: 99%

Immunochemotherapy in American cutaneous leishmaniasis: immunological aspects before and after treatment

Toledo

Mayrink

Gollob

et al. 2001

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

“…Studies indicate the importance of the balance between T CD4 + and CD8 + cells, in which the first ones would have their proportion reduced during the healing process (10,11). Meanwhile, natural killer (NK) cells participate by secreting IFN-γ, important for the differentiation of Th1 and Th2 responses by CD4 + T cells, and also through direct cytotoxic effect on parasitized macrophages (12).…”

Section: Short Communicationmentioning

confidence: 99%

Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil

Almeida¹,

Castro²,

Oliveira³

et al. 2011

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

+ and CD25 + T cells in the peripheral blood of patients afflicted with American cutaneous leishmaniasis (ACL) -before and after treatment -and healthy controls. Peripheral blood was collected and transferred to cytometry tubes containing monoclonal antibodies specific for cell surface markers CD3, CD4, CD8, CD16 e CD25. The immunophenotypic and morphometric parameters of cells were determined by flow cytometry and the results demonstrated a significant increase in the number of T CD8 + cells after treatment, suggesting a cytotoxic T cell response. An increase in CD25 + T cells in patients with active ACL and after treatment was also observed, suggesting the participation of these cells in the modulation of the exacerbated effector response.

show abstract

Immunologic patterns associated with cure in human American cutaneous leishmaniasis

Cited by 46 publications

References 19 publications

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis

Immunochemotherapy in American cutaneous leishmaniasis: immunological aspects before and after treatment

Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil

Contact Info

Product

Resources

About