ImmunoInertial microfluidics: A novel strategy for isolation of small EV subpopulations

Bazaz, Sajad Razavi; Zhand, Sareh; Salomon, Robert N.; Beheshti, Elham Hosseini; Jin, Dong-Yan; Warkiani, Majid Ebrahimi

doi:10.1016/j.apmt.2022.101730

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…EVs play a critical role in intercellular communication, and the separation of EVs is an essential step to use them as biomarkers in various diseases and immunotherapies. 32,33 In this work, we employed the developed viscoelastic microfluidic device to separate EVs depending on their sizes. The migration behaviour of the EVs is similar to the polystyrene particles because they have a similar spherical shape and are close in density.…”

Section: Size-based Separation Of Evsmentioning

confidence: 99%

Viscoelastic microfluidics for enhanced separation resolution of submicron particles and extracellular vesicles

Hettiarachchi,

Ouyang,

Cha

et al. 2024

Nanoscale

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Section: Size-based Separation Of Evsmentioning

confidence: 99%

Viscoelastic microfluidics for enhanced separation resolution of submicron particles and extracellular vesicles

Hettiarachchi,

Ouyang,

Cha

et al. 2024

Nanoscale

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“…There are several applications where continuous-flow microfluidic devices can be used to achieve practical outcomes. These can be quite varied, and range from bacteria separation to prevent beer spoilage [16], exosome subpopulation separation [17], neutrophil purification in diabetes [18], separation of cycling cells [19], dead cell removal [20], and enrichment of CTCs [21] to be used for further multiomics data analysis using single cell methods [22,23]. Additionally, the discovery that CTC clusters play an important role in the metastatic cascade [24][25][26][27][28][29] has created a need to develop devices that can enrich CTC clusters.…”

Section: Quantitative Characterisation Using Beads and Bloodmentioning

confidence: 99%

A Method for Rapid, Quantitative Evaluation of Particle Sorting in Microfluidics Using Basic Cytometry Equipment

Salomon

Bazaz

et al. 2023

Micromachines

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This paper describes, in detail, a method that uses flow cytometry to quantitatively characterise the performance of continuous-flow microfluidic devices designed to separate particles. Whilst simple, this approach overcomes many of the issues with the current commonly utilised methods (high-speed fluorescent imaging, or cell counting via either a hemocytometer or a cell counter), as it can accurately assess device performance even in complex, high concentration mixtures in a way that was previously not possible. Uniquely, this approach takes advantage of pulse processing in flow cytometry to allow quantitation of cell separation efficiencies and resulting sample purities on both single cells as well as cell clusters (such as circulating tumour cell (CTC) clusters). Furthermore, it can readily be combined with cell surface phenotyping to measure separation efficiencies and purities in complex cell mixtures. This method will facilitate the rapid development of a raft of continuous flow microfluidic devices, will be helpful in testing novel separation devices for biologically relevant clusters of cells such as CTC clusters, and will provide a quantitative assessment of device performance in complex samples, which was previously impossible.

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“…Microfluidics, also known as lab-on-a-chip, is the science studying the flow behavior at the microscale. , Flow manipulation within microfluidic devices such as micromixers, − microdroplet generators, − and microseparators − is pursued to achieve the desired output. These systems have found their functionality in various biorelated analyses and engineering. − In many of these applications, efficient and well-controlled mixing is crucial in successfully satisfying the aim of the desired study. , Therefore, micromixers are one of the most pivotal parts of lab-on-a-chip systems.…”

Section: Introductionmentioning

confidence: 99%

A Novel Strategy for Square-Wave Micromixers: A Survey of RBC Lysis for Further Biological Analysis

Hazeri,

Abouei Mehrizi,

Mohseni

et al. 2023

Ind. Eng. Chem. Res.

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Most biorelated applications require homogeneous mixing, necessitating innovative approaches to design high-efficiency micromixers. In this study, for the first time, we have changed the straight structure of a base square-wave micromixer to include regularpolygon-designed mixing units coupled with convergent−divergent channels. The aim was to show that the designed mixing units can enhance mixing at low Reynolds (Re) numbers. Importantly, within the optimization process, the mixing volume and mixing length of the micromixers were kept intact. In this respect, the required numerical evaluations were conducted to obtain the optimized micromixers at Re = 0.1 and 2, where the mixing index for the base micromixer increased from 0.9187 to 0.9944 and from 0.1600 to 0.4043, respectively. Finally, to prove that the optimized micromixers possess a better practical performance than the base micromixer, RBC lysis, as a vital preprocessing step of many biological experiments, was compared at Re = 0.1 and 2 for the base and optimized micromixers.

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ImmunoInertial microfluidics: A novel strategy for isolation of small EV subpopulations

Cited by 5 publications

References 48 publications

Viscoelastic microfluidics for enhanced separation resolution of submicron particles and extracellular vesicles

Viscoelastic microfluidics for enhanced separation resolution of submicron particles and extracellular vesicles

A Method for Rapid, Quantitative Evaluation of Particle Sorting in Microfluidics Using Basic Cytometry Equipment

A Novel Strategy for Square-Wave Micromixers: A Survey of RBC Lysis for Further Biological Analysis

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