Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Sukjumlong, Sayamon; Dalin, Anne-Marie; Sahlin, Lena; Persson, Erik

doi:10.1530/rep.1.00514

Cited by 29 publications

(41 citation statements)

References 49 publications

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“…PGR down-regulation in the pig uterus is initiated by Day 8 and is completed by Day 12 in both cyclic and pregnant animals [4,12,13]. During pregnancy, PGR down-regulation is thought to enable conceptus attachment to the uterine surface and establishment of pregnancy through a mechanism involving the loss of MUC1 [7].…”

Section: Discussionmentioning

confidence: 99%

Uterine Progesterone Receptor Expression, Conceptus Development, and Ovarian Function in Pigs Treated with RU 486 During Early Pregnancy1

Mathew¹,

Sellner²,

Green³

et al. 2011

Biology of Reproduction

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Establishment of pregnancy in the pig depends on down-regulation of progesterone receptor (PGR) in the uterine luminal and glandular epithelium during the first week after breeding. The present study evaluated the regulation of endometrial PGR by progesterone and the possible role of endometrial tumor necrosis factor (ligand) superfamily member 11 (TNFSF11) and nuclear factor-kappa B (NFKB) activation in PGR expression. Mature, cycling gilts were inseminated (Day 0) and assigned to either untreated control (n = 9) or one of two treatments that employed RU 486 to block progesterone action either before (treatment 1 [T1]) or after (treatment 2 [T2]) the initiation of PGR down-regulation. The T1 gilts were treated with RU 486 (400 mg/day) on Days 3-5 of pregnancy (n = 9), and T2 gilts were treated with RU 486 on Days 6 and 7 of pregnancy (n = 9). Uteri and ovaries were collected on Day 8 or 12 of gestation. The diameter of the conceptuses in T1 gilts was approximately half that in controls by Day 8, and normal conceptuses were not collected from any T1 gilts on Day 12. Endometrial PGR mRNA was more abundant in T1 and T2 gilts compared with control gilts. The PGR-B protein decreased from Day 8 to Day 12 in the luminal epithelium and, to some extent, in superficial glandular epithelium in control and T2 gilts. In T1 gilts, the PGR-B protein remained elevated (i.e., failed to undergo down-regulation) on Day 12. Blocking PGR action early in the cycle (i.e., on or before Day 5), therefore, prevented normal conceptus development, caused elevated PGR mRNA, and prevented the decrease in PGR protein that typically occurs in pigs. We could not confirm a role for NFKB activation in PGR down-regulation, because pigs with extreme differences in PGR and TNFSF11 expression (T1 and controls) had similar NFKB activation on Day 8. Activated NFKB within the luminal epithelium and glandular epithelium (both superficial and deep) was observed in T2 and control pigs on Day 12 when elongating conceptuses (presumably releasing interleukin 1 beta to activate NFKB) were recovered. Gilts treated with RU 486 had greater ovarian follicular growth and greater plasma estradiol concentrations. We conclude that the mechanisms controlling PGR down-regulation are progesterone-dependent and occur between Day 3 and Day 6 of pregnancy. NFKB activation did not appear to have a role in PGR down-regulation within the period that we studied. Blocking progesterone action after Day 6 did not reverse the process of PGR down-regulation, nor did it appear to affect the development of conceptuses collected on Day 12.

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Section: Discussionmentioning

confidence: 99%

Uterine Progesterone Receptor Expression, Conceptus Development, and Ovarian Function in Pigs Treated with RU 486 During Early Pregnancy1

Mathew¹,

Sellner²,

Green³

et al. 2011

Biology of Reproduction

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“…1992) and pigs (Geisert et al. 1994; Sukjumlong et al. 2005), where PR is up‐regulated by oestradiol and down‐regulated by progesterone in most uterine cells, including epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Detection of Receptors for Oestrogen and Progesterone in Endometrial Glands and Stroma during the Oestrous Cycle in Nelore (Bos taurus indicus) Cows

Martin

Neto

Oba

et al. 2008

Reprod Domestic Animals

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The aim of the present study was to monitor endometrial distribution and concentrations of oestrogen receptors alpha (ER alpha) and progesterone receptors (PR) by immunohistochemistry in Nelore cows (Bos taurus indicus) during the oestrous cycle. Blood samples were collected for progesterone measurement and endometrial samples were taken from the uterine horn contra lateral to the corpus luteum in 16 cows at days 0 (ovulation), 5, 9, 13 and 19 of the oestrous cycle. Immunostaining evaluation for ER alpha and PR in the glandular epithelium and uterine stroma was performed by two methods: positive nuclei counting and staining intensity of the nuclei. Specific positive staining reactions for both receptors were limited to cell nuclei and they were not identified in the cytoplasm. The proportion of ER alpha positive nuclei had a temporal variation throughout the oestrous cycle in both cell types evaluated and was higher in uterine stroma than the glandular epithelium (p < 0.05). The greatest proportion of ER alpha stained nuclei was observed at oestrus and during the initial and mid luteal phase (days 5, 9 and 13) (p < 0.05) in the glandular epithelium and at days 0, 5 and 9 in the uterine stroma (p < 0.01). The proportion of PR positive nuclei remained constant throughout the entire oestrous cycle for both cell types evaluated (p > 0.05). A higher proportion of PR positive nuclei was measured in the uterine stroma compared with the glandular epithelium (p < 0.05). Intensity of staining for ER alpha and PR varied throughout the oestrous cycle (p < 0.01). There was a higher staining intensity at days 0 and 5 in the stroma for ER alpha (p < 0.01) and PR (p < 0.01) and in the glandular epithelium at days 0, 5, 9 and 13 for ER alpha (p < 0.01) and at days 0, 5 and 9 for PR (p < 0.01) when compared with the other evaluated days. These data demonstrate that ER alpha and PR expression varied throughout the oestrous cycle in Nelore cows, in general with highest concentrations at oestrus and the lowest during the luteal phase. This is similar to patterns observed in Bos taurus taurus.

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“…The sections were deparaffinized in xylene and rehydrated in graded alcohol. The immunohistochemical protocol was modified after Sukjumlong et al. (2005).…”

Section: Methodsmentioning

confidence: 99%

“…2005). Furthermore, Sukjumlong et al. (2005) demonstrated that the PR was higher in inseminated sows compared with cyclic sows, and that the immunostaining of PR in the uterus was high during 5 or 6 to 70 h after insemination.…”

Section: Introductionmentioning

confidence: 96%

“…1996). The physiological mechanism of P 4 on sperm transportation in the female reproductive tract is related to the expression of the progesterone receptor (PR) in the uterine horn of the pig (Sukjumlong et al. 2005) and it has been demonstrated that the PR in the pig oviduct (ampulla and isthmus) was more intense during the luteal phase compared with the follicular phase (Peralta et al.…”

Section: Introductionmentioning

confidence: 99%

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Expression of Progesterone Receptor in the Utero-tubal Junction After Intra-uterine and Deep Intra-uterine Insemination in Sows

Tummaruk¹,

Tienthai

Manee-in

et al. 2009

Reproduction in Domestic Animals

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The aim of this study was to investigate the expression of progesterone receptor (PR) in the utero-tubal junction (UTJ) of sows at 24 h after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared with conventional artificial insemination (AI) in pigs. Fifteen multiparous sows were used: AI (n = 5), IUI (n = 5) and DIUI (n = 5). The sows were inseminated with a single dose of diluted semen during the second oestrus after weaning at 6-8 h prior to ovulation (AI: 3000 × 10(6) spermatozoa, IUI: 1000 × 10(6) spermatozoa and DIUI: 150 × 10(6) spermatozoa). The UTJ was collected and subject to immunohistochemical staining using avidin-biotin immunoperoxidase technique with mouse monoclonal antibody to PR. In the oviductal part of the UTJ, the intensity of PR in the tunica muscularis and the proportion of PR-positive cells in the surface epithelium after DIUI were lower than AI (p < 0.05). The intensity and the proportion of PR-positive cells between AI and IUI in all compartments of the UTJ did not differ significantly (p > 0.05). When comparing between tissue compartments, prominent staining was observed in the muscular layer of the UTJ. It could be concluded that the expression of PR in the UTJ prior to fertilization after DIUI with a reduced number of spermatozoa was lower than that after AI. This might influence sperm transportation and the fertilization process.

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Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Cited by 29 publications

References 49 publications

Uterine Progesterone Receptor Expression, Conceptus Development, and Ovarian Function in Pigs Treated with RU 486 During Early Pregnancy1

Uterine Progesterone Receptor Expression, Conceptus Development, and Ovarian Function in Pigs Treated with RU 486 During Early Pregnancy1

Immunohistochemical Detection of Receptors for Oestrogen and Progesterone in Endometrial Glands and Stroma during the Oestrous Cycle in Nelore (Bos taurus indicus) Cows

Expression of Progesterone Receptor in the Utero-tubal Junction After Intra-uterine and Deep Intra-uterine Insemination in Sows

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