Expression of Progesterone Receptor in the Utero-tubal Junction After Intra-uterine and Deep Intra-uterine Insemination in Sows

Abstract

The aim of this study was to investigate the expression of progesterone receptor (PR) in the utero-tubal junction (UTJ) of sows at 24 h after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared with conventional artificial insemination (AI) in pigs. Fifteen multiparous sows were used: AI (n = 5), IUI (n = 5) and DIUI (n = 5). The sows were inseminated with a single dose of diluted semen during the second oestrus after weaning at 6-8 h prior to ovulation (AI: 3000 × 10(6) spermatozoa, IUI: 1000 × 10(6) spermatozoa and DIUI: 150 × 10(6) spermatozoa). The UTJ was collected and subject to immunohistochemical staining using avidin-biotin immunoperoxidase technique with mouse monoclonal antibody to PR. In the oviductal part of the UTJ, the intensity of PR in the tunica muscularis and the proportion of PR-positive cells in the surface epithelium after DIUI were lower than AI (p < 0.05). The intensity and the proportion of PR-positive cells between AI and IUI in all compartments of the UTJ did not differ significantly (p > 0.05). When comparing between tissue compartments, prominent staining was observed in the muscular layer of the UTJ. It could be concluded that the expression of PR in the UTJ prior to fertilization after DIUI with a reduced number of spermatozoa was lower than that after AI. This might influence sperm transportation and the fertilization process.

Conception Rate and Litter Size in Multiparous Sows after Intrauterine Insemination Using Frozen-Thawed Boar Semen in a Commercial Swine Herd in Thailand

“…On the other hand, for deep intrauterine insemination, the distribution of sperm in the female reproductive tract may not be enough in some sows, if too low of a number of sperm is used (<0.15 × 10 9 sperm/dose). Furthermore, it has been demonstrated that the progesterone receptor in the sperm reservoir of sows inseminated with deep intrauterine insemination with a low number of sperm differs from that inseminated by a conventional artificial insemination technique [18]. This may also influence fertility and/or fecundity of the sows.…”

No association between triple‑negative breast cancer and prognosis of patients receiving breast‑conserving treatment

“…IHC and FISH analysis were conducted on tissue sections (3–4 µm) and tissue microarray slides. IHC (Benchmark XT; Ventana Medical Systems, Inc., Tucson, AZ, USA) was performed on formalin-fixed and paraffin-embedded tissues of 757 patients using the avidin-biotin-immunoperoxidase technique ( 22 ). Tissue sections were deparaffinized in xylene and rehydrated in a graded series of ethanol.…”