Immunohistochemical profile of integrins in enlarged dental follicles and dentigerous cysts

Godoy, Gustavo Pina; Silveira, Éricka Janine Dantas da; Lins, Ruthinéia Diógenes Alves Uchôa; Souza, Lélia Batista de; Freitas, Roseana de Almeida; Queiroz, Lélia Maria Guedes

doi:10.1016/j.tripleo.2007.05.024

Cited by 10 publications

(10 citation statements)

References 18 publications

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“…Godoy et al ., applied the following histopathological criteria: Observation of a thin stratified squamous epithelial lining of the cystic cavity, exhibiting areas of continuity and arranged throughout a connective capsule of variable density; The criteria for DF included: observation of a thin and discontinuous simple cuboidal reduced enamel epithelium, along with loose fibrous connective tissue caspule. [19] Da Silva T A espoused the following criteria for DCs: (a) A predominant lining by stratified squamous epithelium, (b) pericoronal spaces >5.6 mm, and (c) surgical exploration revealing bone cavitation and cystic content. DFs were predominantly lined by reduced enamel epithelium, the width of the pericoronal space was <5.6 mm, and no bone cavitations or cystic content was detected.…”

Section: Discussionmentioning

confidence: 99%

Histopathologic evaluation of follicular tissues associated with impacted lower third molars

Tegginamani

Prasad

2013

J Oral Maxillofac Pathol

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Context:Previous studies have reported that the dental follicular tissues associated with impacted lower third molars (ILTMs) may undergo cystic degeneration and/or neoplastic transformation. This is especially likely when the pericoronal space is >2.5 mm on intraoral radiographs and >3 mm on panoramic radiographs and to examine dental follicular tissue for pathological changes in patients with ILTMs and pericoronal radiolucencies of <2.5 mm.Aim:Histopathological evaluation of follicular tissues associated with ILTMs.Materials and Methods:The morphology of the hematoxylin and eosin-stained follicular tissues of 146 such impactions were studied.Results:On microscopy, no cystic structures with fibrous walls were identified. 85 cases (58%) showed fibrous or myxomatous connective tissue and no epithelial elements. 61 cases (42%) showed epithelial elements in addition to fibrocollagenous tissue. Of these, 16 cases exhibited epithelium, of which 13 cases showed reduced enamel epithelium and three cases showed squamous metaplasia/non-keratinized stratified squamous epithelium.Conclusions:All asymptomatic unerupted third molars with pericoronal radiolucency of <2.5 mm should be retained since they do not exhibit cyst formation microscopically.

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Section: Discussionmentioning

confidence: 99%

Histopathologic evaluation of follicular tissues associated with impacted lower third molars

Tegginamani

Prasad

2013

J Oral Maxillofac Pathol

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“…The use of cell proliferating markers, such as integrins (Godoy et al, 2007), Ki-67 (Cabbar et al, 2008;Edamatsu et al, 2005) and PCNA (Adelsperger et al, 2000), has shown that the squamous metaplasia would be the first visible sign of cystic transformation of the DF and not a normal change due to advanced age. In our study, although we have not found a statistical association between PTCH labeling and the type of epithelial lining, probably due to the low number of PTCH-positive cells, the specimens with squamous metaplasia had higher rates of positivity for PTCH than DF with reduced enamel epithelium.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Human Homologue of Drosophila Patched (PTCH) in Dental Follicles of Impacted Third Molars

et al. 2012

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SUMMARY:This study investigated the immunodetection of PTCH in epithelial components of dental follicles associated with impacted third molars without radiographic signs of pathosis. One hundred and five specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferation was determined by using immunohistochemical labeling. Statistical analysis was performed using Fisher exact test and a level of significance of 5%. Of the 105 dental follicles collected, 3 were PTCH-positive. The specimens with squamous metaplasia and epithelial hyperplasia had higher rates of positivity for PTCH, as well as those with active remnants of odontogenic epithelium. This study suggests that the odontogenic cells of the dental follicle might be proliferating during the rhizogenesis, while the squamous metaplasia and hyperplasia of the epithelial lining and proliferative odontogenic epithelial rests show the differentiation potential of dental follicles.

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“…The use of cell proliferating markers, such as integrins (Godoy et al, 2007), Ki-67 (Edamatsu et al, 2005;Cabbar et al, 2008) and PCNA (Adelsperger et al), has shown that the squamous metaplasia would be the first visible sign of cystic transformation of the DF and not a normal change due to advanced age. In our study, although we have not found a statistical association between PCNA labeling and the type of epithelial lining, probably due to the low number of PCNA-positive cells, the specimens with squamous metaplasia had higher rates of positivity for PCNA than DF with reduced enamel epithelium.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Proliferating Cell Nuclear Antigen (PCNA) in Dental Follicles of Impacted Third Molars

Oliveira

Silveira²,

Andrade³

et al. 2011

Int. J. Morphol.

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SUMMARY:This study investigated the immunodetection of PCNA in epithelial components of dental follicles associated with impacted third molars without radiographical and morphological signs of pathosis. A total of 105 specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferating was determined by using immunohistochemical labeling. Statistical analysis was performed using the Fisher exact test. Of the 105 dental follicles collected, 6 were PCNA-positive (≈ 6%). The specimens with squamous metaplasia and epithelial hyperplasia had higher rates of positivity for PCNA, as well as those with proliferative remnants of odontogenic epithelium. In conclusion, this study shows that dental follicles at this stage of development have low proliferative potential, but suggests that squamous metaplasia, hyperplasia of the epithelial lining and presence of proliferative odontogenic epithelial rests in the connective tissue may be early signs of developing lesions of odontogenic origin.

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Immunohistochemical profile of integrins in enlarged dental follicles and dentigerous cysts

Cited by 10 publications

References 18 publications

Histopathologic evaluation of follicular tissues associated with impacted lower third molars

Histopathologic evaluation of follicular tissues associated with impacted lower third molars

Immunohistochemical Analysis of Human Homologue of Drosophila Patched (PTCH) in Dental Follicles of Impacted Third Molars

Immunohistochemical Analysis of Proliferating Cell Nuclear Antigen (PCNA) in Dental Follicles of Impacted Third Molars

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