Immunohistochemical Parameters of Musashi-1 in Nodular and Diffuse Adenomyosis

Abstract

Comparative immunohistochemical analysis of the expression of somatic stem cells marker Musashi-1 in the endometrium was performed during various phases of menstrual cycle and in patients with nodular and diffuse adenomyosis. The expression of Musashi-1 reflecting proliferative potential of epithelial and stromal cells was quantitatively analyzed by the optical density in the nuclei and cytoplasm of epithelial and stromal cells of the eutopic endometrium and adenomyosis foci. In the eutopic endometrium, the immunohistochemical reaction was more intensive during proliferation phase in comparison with secretion phase. Enhanced expression of Musashi-1 was observed in adenomyosis foci in comparison with endometrial cells. The most intensive staining was found in nodular adenomyosis, especially in epithelial cells during the secretion phase. These data attest to the role of somatic stem cells in the development and progression of various forms of adenomyosis.

Exosomes: potential diagnostic markers and drug carriers for adenomyosis

“…Several studies confirmed that the pathogenesis of AM is associated with the host immune responses ( Kozachenko et al, 2017 ; Bourdon et al, 2021 ). Activation of the immune system of AM patients leads to the release of a plethora of cytokines, which induces changes in the local immune microenvironment and ultimately influences the development of AM.…”

Bioinformatics strategy for the screening of key genes to differentiate adenomyosis from endometriosis (Review)

“…MSI1 reduces the expression of p21 CIP and promotes cell proliferation and cell division. MSI1 expression has been shown to be increased in adenomyosis (119) and endometriosis (120).…”