Immunohistochemical expression of p53 and its clinicopathological correlation with modified Anneroth′s histological grading system

Abstract

Introduction and Objectives:Oral squamous cell carcinoma (OSCC) is an epithelial neoplasm generally beginning as focal overgrowth of altered stem cells near the basement membrane, moving upward and laterally, replacing the normal epithelium. Histopathological grading has been used for many decades in an attempt to predict the clinical behavior of oral squamous cell carcinoma. In the present study, Forty biopsies were studied for histological grading and p53 expression. The p53 expression was studied in relation to clinical parameters such as age, sex of patient and site of tumors. Relation between histological grade of malignancy and p53 protein expression was analysed. All cases were classified according to Anneroth's histological malignancy grading system (1987).Materials and Methods:40 cases of OSCC were assessed for clinical parameters, Anneroth's histological grading and immunohistochemically stained with p53 protien.Statistical Analysis:The results obtained were analyzed using Spearman's Co-relation.Observations and Results:The positive expression of p53 was found in 62% of carcinomas studied. Positivity of p53 showed correlation with histological grade of malignancy and with individual parameters like degree of keratinization, nuclear polymorphism, number of mitoses and lymphoplasmacytic infiltration while showed a negative correlation with pattern of invasion.Conclusion:Our study showed a significant correlation between parameters of tumor cell population, lymphoplasmacytic infiltration and p53 expression. A significant association between high grade of malignancy and p53 overexpression and insignificant correlation of p53 with age, sex of the patient and site of the tumor was found.

PDGFRα/HER2 and PDGFRα/p53 Co-expression in Oral Squamous Cell Carcinoma

“…Therefore, it seems reasonable to assume that high p53 protein expression levels detected by IHC reflect the extended half-life of p53 protein encoded by TP53 gene mutation (3). Similarly to published studies (3,14,15) we found correlation between p53 overexpression and grade of OSCC. The authors postulated that patients with OSCC whose tumor showed high expression of p53 protein had worse clinicopathological parameters and presented aggressive behavior (3,14).…”

“…The loss of p53 function with resultant overexpression has been reported in various human tumors such a breast, brain, rectum, colon, oesophagus, lung cancers and OSCCs (4). Previous studies have shown that oral carcinomas with overexpression/mutation of p53 grow faster and have aggressive nature and poor prognosis (1,3,15).…”

Immunohistochemical over expression of p53 in head and neck Squamous cell carcinoma: clinical and prognostic significance

“…Previous literature also showed association of p53 expression with tumor grade and other histologic features. Dave et al in a study involving 40 cases of HNCC found a significant association of p53 expression with tumor grade and other histologic parameters like degree of keratinization [ 11 ]. Although we found significant association of p53 expression with tumor grade, however association with other histologic parameters was not noted.…”

Broder versus Bryne's histologic grading parameters on incision biopsy specimens

“…P53 in proven to be involved in apoptosis and cell-cycle control making it a plausible biomarker of malignant potential. [ 8 9 10 ] Ki-67 can be detected in phases G1, S, G2 and M of the cell cycle, but not on G0 phase, exclusively in the nuclei of cycling cells making it a reliable marker of proliferating cells. [ 2 ]…”

“…in which 65% expression of p53 was found. [ 9 ] Their study showed significant correlation of grading parameters like the degree of keratinization, nuclear pleomorphism, number of mitosis and lymphoproliferative infiltrate with p53 expression. The increasing P53 expression along the increasing grade of tumor severity is proven in many studies.…”