Broder versus Bryne's histologic grading parameters on incision biopsy specimens: A comparative study with P53 and KI67 expression

Abstract

Objective: Various histological grading of oral squamous cell carcinoma has been contributed to the literature at different periods, but the reliability of such grading systems is controversial. This study attempted to measure the efficacy of Bryne's parameters on the full thickness of incisional biopsies which are representative of the original lesion with the proven molecular malignancy markers P53 and Ki67. Materials and Methods: One hundred incisional biopsy specimens of oral squamous cell carcinoma were obtained and histologically graded according to Broder's grading system. The same was graded using Bryne's parameters on full thickness of obtained incision sample. Immunohistochemistry was carried out for both p53 and Ki67. Results: We found a high discrepancy in the grading of lesions with Broader as well as Bryne grading parameters within the same lesion. When compared with the molecular expression percentages of p53 and Ki67, highly significant correlation was found in Bryne's parameters (Pearson Chi-square, P value 0.000) in incisional biopsies than Broder's. Conclusion: The use of parameters by Bryne on invasive front of excision biopsies can provide significant grading on incisional biopsies which are more comparable to the molecular behavior of tumor given by the p53 and Ki67 expression.

Tumor-stroma contact ratio - a novel predictive factor for tumor response to chemoradiotherapy in locally advanced oropharyngeal cancer

“…Extensive in vivo data from human carcinomas demonstrates the critical role the activated tumor stroma plays in tumor growth and treatment response. In particular, the pattern of tumor invasion, driven by interactions between malignant epithelial cells and activated fibroblasts, creates particular tumor microenvironments (TME) and reflects the invasive potential of squamous cell carcinomas (SCC) of the head and neck region [ [4] , [5] , [6] , [7] , [8] , [11] , [12] , [13] , [14] ]. Hence, it would be desirable to have an objective, reproducible and quantitative measure representing the shape of the tumor-stroma interface to enable correlation with other pathological characteristics of the tumor (e.g., biomarker expression or immune cell composition) and clinical outcome data.…”

“…Consequently, risk stratification scores incorporating invasion patterns have been proposed to guide adjuvant radiotherapy decisions in OSCC [ 3 , 4 ]. Despite comparative studies demonstrating the superior prognostic value of grading systems that integrate invasion patterns, these factors have not yet been adopted by the WHO grading system, which still relies primarily on differentiation-based parameters proposed by Broders in 1920 [ [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] ].…”

Prevalence and anatomopathological characterization of cutaneous squamous cell carcinomas with regional and distant metastases in dogs and cats: 20 cases (1985–2020)

“…Studies evaluating incisional samples of oral SCCs in humans demonstrate that the classification according to Bryne's (1992) when used in association with molecular markers (Ki‐67, E‐cadherin and p53) presents more reliable parameters in the classification of the tumour in relation to the Broder system, considering the molecular behaviour of the SCC 22 . However, in cutaneous 23 and subungual 24 SCCs, this finding still requires further studies.…”