Immunohistochemical expression of caspase-3, caspase-5, caspase-7 and apoptotic protease-activating factor-1 (APAF-1) in the liver and kidney of rats exposed to zoledronic acid (ZOL) and basic fibroblast growth factor (bFGF)

Aydoğan, Ahmet; Koçer, Gülperi; Özmen, Özlem; Koçer, Murat; Önal, Levent; Koşkan, Özgür

doi:10.1080/01652176.2014.928759

Cited by 4 publications

(4 citation statements)

References 41 publications

(47 reference statements)

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“…17 Caspase-3, a pivotal role in apoptosis, is a downstream effector and is affected by intrinsic and extrinsic pathways. 25,26 Various stimuli can affect the apoptosis. Calcium ion plays a crucial role in the intrinsic apoptotic pathway: high regulation of calcium into mitochondria lead to the deliverance of cytochrome c. 16 In the cytoplasm, cytochrome c ties to a cytoplasmic protein known as APAF-1 and shapes the apoptosome that initiates procaspase-9 into caspase-9 and then triggers other caspases that will eventually execute cell disintegration.…”

Section: Discussionmentioning

confidence: 99%

“…23 Most caspases have roles in cell proliferation, survival, inflammation, or differentiation. 25 Other than lethal function in apoptosis, caspase-3 and caspase-9 have non-lethal function in cell differentiation, with caspase-3 having more functions in cell maturation and activation. 23 Caspase-3 has a vital role in tissue regeneration, differentiation, and neural development differently and not involving apoptotic activity.…”

Section: Discussionmentioning

confidence: 99%

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Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

Prasetyo

Kuntjoro

Goenharto

et al. 2021

CCIDE

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Purpose Calcium hydroxide is a gold standard dental material generally used for pulpal and periapical therapy including regenerative endodontic procedures because of its positive properties. However, evaluation about this material on stem cells is limited. Human umbilical cord mesenchymal stem cells (HUCMSCs) are potential to be used in regenerative therapy. Regenerative therapy needs a sustainable cell supply to maintain its regenerative capacity. The aim of this study was to ascertain the apoptosis result of calcium hydroxide on HUCMSCs through the expression of apoptotic protease-activating factor-1 (APAF-1), caspase-3, and caspase-9. Materials and Methods This study used a thawed frozen stock of passage 5 HUCMSCs, grown in minimum essential medium (MEM) alpha containing calcium hydroxide at concentration of 0.1 microgram/mL for 1, 3 and 7 days. Polyclonal antibody with fluorescence isothiocyanate (FITC) label was used to evaluate the expressions. APAF-1, caspase-3, and caspase-9 expressions were recorded and compared on every observation day using fluorescence microscope. Analysis of variance was performed to analyze the significance among the results of treatment groups. The results were concluded significant if p<0.05. Results The addition of calcium hydroxide in MEM alpha medium increases HUCMSCs expression of APAF-1, caspase-3 and caspase-9 significantly, compared to the control group without calcium hydroxide (p<0.05) in all the times. Day 1 showed the lowest increase followed by higher expressions on day 3 and day 7. Conclusion HUCMSCs express increased APAF-1, caspase-3 and caspase-9 after in-vitro calcium hydroxide exposure. This should be considered when using calcium hydroxide on HUCMSCs for regenerative procedures with regard to other positive properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

Prasetyo

Kuntjoro

Goenharto

et al. 2021

CCIDE

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“…Zoledronate, a nitrogen-containing bisphosphonate, has the effect that selectively induces apoptosis on mononuclear macrophages. There was a study that found that zoledronate liposomes treatment could significantly increase the apoptosis in the liver of rats [29]. Liposome is an ideal drug carrier, which is circulated in the blood system and mainly phagocytized by the reticuloendothelial system of the liver and spleen.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Apoptosis of Kupffer Cells Induced by Zoledronate Liposomes Following Hepatic Ischemia-Reperfusion Injury

Zhao

Han

et al. 2018

Ann Transplant

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BackgroundThe goal of this study was to observe the effect of the apoptosis of Kupffer cells (KCs) selectively induced by zoledronate liposomes following the hepatic ischemia-reperfusion injury (IRI) in the rat liver transplantation model and to explore its mechanisms.Material/MethodsThe rat liver transplantation model was established using the improved Kamada method. Male Sprague Dawley rats were randomly divided into 3 groups: no liver transplantation or drug treatment (Group A); donor rats were injected with 1 mL normal saline through the tail vein for 3 continuous days before transplantation, and the donor liver was preserved in cold for 2 hours (Group B); donor rats were injected with 1 mL zoledronate liposomes (0.001 mg/mL) through the tail vein for 3 continuous days before transplantation, and the donor liver was preserved in cold for 2 hours (Group C). At 24 hours after transplantation, the receiving rats were sacrificed for sampling.ResultsCompared with Group C and Group A, the bile secretion flow was dramatically decreased in Group B, whereas the serum liver function index [alanine aminotransferase (ALT), glutamate aminotransferase (AST), and γ-glutamyl transpeptidase (γ-GT)] was significantly increased (P<0.01), and the pathological injury area was obviously increased. Compared with Group B, the levels of serum interleukin1 (IL-1), tumor necrosis factor-α (TNF-α), and the apoptotic index in Group C were significantly decreased (P<0.05), and Suzuki scores of congestion, vacuolar degeneration, and necrosis were all reduced (P<0.05).ConclusionsThe apoptosis of KCs selectively induced by zoledronate liposomes inhibited the inflammatory cascade reaction induced by KC activation and reduced the release of cytokines and decreased the extent of IRI in the liver transplantation in animal model.

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“…The findings of immunohistochemical expression of caspase were scored as weak (less than 10% of myocytes) immune-positive reaction or focal cytoplasmic expression of caspase-3, mild (30-45% of myocytes), mild to moderate (50-55% of myocytes), strong (60-65% of myocytes) [33].…”

Section: Primer Namementioning

confidence: 99%

Cardioprotective effect of Ginger in a rat model of myocardial damage and its possible intervention in PERK-ATF4-CHOP-PUMA apoptotic pathway

Mohammed¹,

Osman²,

Mourad³

et al. 2023

Ukr.Biochem.J.

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For today the exact mechanisms of myocardial infarction and ischemia/reperfusion injury are still not fully understood. ER stress and integrated stress response pathways are thought to play an essential role in myocardial damage. This includes activation of endoplasmic reticulum kinase PERK, induction of activating transcription factor ATF4, expression of pro-apoptotic transcription factor CHOP and P53 up-regulated modulator of apoptosis (PUMA) involved in apoptosis control. We used a rat model of isoproterenol-induced myocardial damage to elucidate the possible cardioprotective effect of Ginger through the influence on ER stress-induced apoptotic pathway. We also compared its effect with the ACE inhibitor Captopril. Male albino Wistar rats received 1.0 or 2.0 ml of Zingiber officinale (Ginger) powder suspension (200 mg/ml) daily by intra-gastric intubation for 28 days. Isoproterenol at a dose of 85 mg/kg was IP injected on the 27th and 28th days. Serum aspartate transaminase (AST) level was measured using kinetic kit. Heart tissue was used for RNA extraction, evaluation of gene expression by Q-RT-PCR, immuno-histochemical determination of caspase-3 expression and histopathological studies. Our results showed that Isoproterenol administration increased CHOP-mRNA expression 4 folds in cardiac muscle tissue compared to normal control. Ginger pretreatment significantly decreased both CHOP and ATF4, and PUMA mRNA expression compared to Isoproterenol-treated groups. A significant reduction in ATF4 mRNA expression in a group pretreated with Captopril and Ginger compared to normal control group was observed. The results showed that Ginger reduced AST serum levels which correlated with results of histopathological studies of heart tissue. Our findings suggest that the protective effects of Ginger against myocardium damage induced by Isoproterenol may be mediated by reducing the endoplasmic reticulum stress by affecting the ATF4-CHOP-PUMA pathway. Keywords: AST, ATF4, Captopril, CHOP, Ginger, ischemia-reperfusion, Isoproterenol, myocardial damage, PUMA

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Immunohistochemical expression of caspase-3, caspase-5, caspase-7 and apoptotic protease-activating factor-1 (APAF-1) in the liver and kidney of rats exposed to zoledronic acid (ZOL) and basic fibroblast growth factor (bFGF)

Cited by 4 publications

References 41 publications

Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

Calcium Hydroxide Increases Human Umbilical Cord Mesenchymal Stem Cells Expressions of Apoptotic Protease-Activating Factor-1, Caspase-3 and Caspase-9

Protective Effects of Apoptosis of Kupffer Cells Induced by Zoledronate Liposomes Following Hepatic Ischemia-Reperfusion Injury

Cardioprotective effect of Ginger in a rat model of myocardial damage and its possible intervention in PERK-ATF4-CHOP-PUMA apoptotic pathway

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