Purpose Calcium hydroxide is a gold standard dental material generally used for pulpal and periapical therapy including regenerative endodontic procedures because of its positive properties. However, evaluation about this material on stem cells is limited. Human umbilical cord mesenchymal stem cells (HUCMSCs) are potential to be used in regenerative therapy. Regenerative therapy needs a sustainable cell supply to maintain its regenerative capacity. The aim of this study was to ascertain the apoptosis result of calcium hydroxide on HUCMSCs through the expression of apoptotic protease-activating factor-1 (APAF-1), caspase-3, and caspase-9. Materials and Methods This study used a thawed frozen stock of passage 5 HUCMSCs, grown in minimum essential medium (MEM) alpha containing calcium hydroxide at concentration of 0.1 microgram/mL for 1, 3 and 7 days. Polyclonal antibody with fluorescence isothiocyanate (FITC) label was used to evaluate the expressions. APAF-1, caspase-3, and caspase-9 expressions were recorded and compared on every observation day using fluorescence microscope. Analysis of variance was performed to analyze the significance among the results of treatment groups. The results were concluded significant if p<0.05. Results The addition of calcium hydroxide in MEM alpha medium increases HUCMSCs expression of APAF-1, caspase-3 and caspase-9 significantly, compared to the control group without calcium hydroxide (p<0.05) in all the times. Day 1 showed the lowest increase followed by higher expressions on day 3 and day 7. Conclusion HUCMSCs express increased APAF-1, caspase-3 and caspase-9 after in-vitro calcium hydroxide exposure. This should be considered when using calcium hydroxide on HUCMSCs for regenerative procedures with regard to other positive properties.
Adhesive dentistry’s main assumption is to create a strong chemical bond between dental hard tissues and restorative composite material. One of the most important aspects of this interface is the hybrid layer. Unfortunately, due to physical and chemical causes, the hybrid layer wears away with time. Epigallocatechin-3-gallate (EGCG), a component extracted from green tea, has several roles in the medical and dentistry field including as a crosslinking agent and as a chelating agent. Although there are several negative results, EGCG was proven to be able to preserve resin-dentin bonds without harming the restoration. As a crosslinking agent and chelating agent, EGCG has the potential to enhance the physical properties of dentin collagen and resin-dentin adhesion. The purpose of this study was to see how EGCG, as a cross-linking agent, affected dentinal collagen and hybrid layers, as well as how chelating chemicals affected Matrix metalloproteinase (MMPs).
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