1989
DOI: 10.1007/bf00822024
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Immunohistochemical differentiation between lymphangiographically verified lymphatic vessels and blood vessels

Abstract: In order to investigate the antigen profile in human lymphatic vessels when compared with blood vessels, postmortem retrograde lymphangiography was done via the thoracic duct on six patients. Formalin fixed, paraffin embedded tissue was stained immunohistochemically for Factor VIII-Related antigen (F VIII R:Ag), with Ulex Europaeus 1 lectin (UEA-1) and for laminin. The results show that the endothelium of blood vessels and lymphatics at all levels of the lymphatic system react positively following staining for… Show more

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Cited by 28 publications
(17 citation statements)
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“…Our immunoperoxidase method using laminin and type IV collagen antisera reveals clearly different immunoreactivity to these antisera between lymphatic capillaries and blood capillaries: lymphatic capillaries have negative or weak immunoreactivity around the wall in contrast to blood capillaries with strong, linear subendothelial staining. These findings are in accord with the results reported by Barsky (3), Autio-Harmainen (2) and Hultberg (10), and are clearer in our IGSS method than in the immunoperoxidase method. Fine granular products in the IGSS method, using 5 nm immunogold particles, were in sharper contrast against the background, and were more intense and thinner than DAB reaction products in the immunoperoxidase method.…”
Section: Discussionsupporting
confidence: 94%
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“…Our immunoperoxidase method using laminin and type IV collagen antisera reveals clearly different immunoreactivity to these antisera between lymphatic capillaries and blood capillaries: lymphatic capillaries have negative or weak immunoreactivity around the wall in contrast to blood capillaries with strong, linear subendothelial staining. These findings are in accord with the results reported by Barsky (3), Autio-Harmainen (2) and Hultberg (10), and are clearer in our IGSS method than in the immunoperoxidase method. Fine granular products in the IGSS method, using 5 nm immunogold particles, were in sharper contrast against the background, and were more intense and thinner than DAB reaction products in the immunoperoxidase method.…”
Section: Discussionsupporting
confidence: 94%
“…Fine granular products in the IGSS method, using 5 nm immunogold particles, were in sharper contrast against the background, and were more intense and thinner than DAB reaction products in the immunoperoxidase method. Lymphatic collecting vessels showed weak focal staining around the wall as reported in dermal lymphatics (2) and an especially intense reaction in the areas near valves (10). These findings of lymphatic vessels were in good agreement with our conventional electron microscopy demonstration that disrupted lamina densa was occasionally present around lymphatic collecting vessels, and especially valve sites had a continuous lamina densa.…”
Section: Discussionsupporting
confidence: 91%
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“…Enzyme-histochemical procedures for 5'-nucleotidase and alkaline phosphatase have been introduced [13], but their specificity for the lymphatic endothelium is not well confirmed [14]. Recently, immunohistochemical staining for factor VIII-related antigen , Ulex europaeus I lectin (UEA-I) binding [15,16], LN and IV [14,15,18,19] have been found to be useful in discriminating blood vessels from lymphatics in humans or rats. Vascular invasion was also well demonstrated by these stains [11,[20][21][22][23].…”
Section: Discussionmentioning
confidence: 98%
“…The vWF is a marker of blood vascular and lymphatic endothelium and is therefore regarded as a panendothelial marker. The lymphatic endothelium shows a weaker intensity and more focal staining when compared with blood vascular endothelium because of the sparse and attenuated cytoplasm of the lymphatic endothelial cells [13,28,30].…”
Section: Distinction Between Blood Vessels and Initial Lymphatics: Anmentioning
confidence: 99%