Immunohistochemical demonstration of keratins in the cysts of thyroid glands, parathyroid glands, and C‐cell complexes of the dog

Abstract

Cyst structures were often detected in and around thyroid glands of the dog. The present study revealed the frequency of occurrence, the light microscopic features, and the immunoperoxidase reactions to anti-keratin and anti-19S-thyroglobulin antisera of each cyst located in parathyroid III, parathyroid IV, thymus IV, C-cell complexes, and thyroid parenchyma from 112 dogs. In each location, cysts showed characteristic features. In parathyroid III, the cysts were covered with single or pseudostratified epithelium composed of ciliated cells; whereas in parathyroid IV they were covered with keratinizing stratified squamous epithelium. In C-cell complexes, small cysts lined with small packed cells were predominant, and large cysts lined with single cuboidal cells or stratified squamous cells were also present. In thymus IV located in the close vicinity of parathyroid IV, cyst epithelium consisted of several types of cells showing variable features. In thyroid parenchyma, there were several types of cysts: some were covered with ciliated columnar cells, and others were covered with two or multilayers of small packed cells or cuboidal cells. In spite of these differences in appearance of the cysts located in different tissues, all their epithelia were immunoreactive to the keratin antisera, except for small cysts in C-cell complexes, which were regarded as immature structures. Thus, the presence of keratin filaments in epithelial cells seems to be a characteristic feature of all cysts. The lumens of each cyst contained variable amounts of amorphous materials, which showed colloid-like, flocculent, foamy, and granular features and were periodic acid-Schiff-positive in variable degrees, from weak to intense. Although the lumenal contents of the cysts in parathyroid III revealed no immunoreactivity for 19S-thyroglobulin, those in thyroid parenchyma, C-cell complexes, parathyroid IV, and thymus IV reacted strongly with the 19S-thyroglobulin antiserum.

Co-expression of vimentin and 19S-thyroglobulin in follicular cells located in the C-cell complex of dog thyroid gland.

“…Both the dog C-cell complexes and the chicken ultimobranchial glands are characterized by the presence of cystic structures (Kameda, 1971(Kameda, ,1984. Furthermore, cysts are observed in the thyroid parenchyma, parathyroid glands 111 and IV, and thymus IV located adjacent to the parathyroid IV in dogs (Kameda, 1987). All cyst epithelia located in these tissues are densely immunostained with the antiserum to epidermal keratins with 50,000-and 58,000-dalton molecular masses (Kameda, 1987;Kameda et al, 1986).…”

“…Furthermore, cysts are observed in the thyroid parenchyma, parathyroid glands 111 and IV, and thymus IV located adjacent to the parathyroid IV in dogs (Kameda, 1987). All cyst epithelia located in these tissues are densely immunostained with the antiserum to epidermal keratins with 50,000-and 58,000-dalton molecular masses (Kameda, 1987;Kameda et al, 1986). Thyroid follicular cells display well-developed cytoskeletons (Takasu et al, 1988).…”

Differential distribution of S-100 protein and vimentin in the hypophyseal pars tuberalis of the guinea pig.

“…The vimentin MAb and the polyclonal anti-GFAP antiserum were purchased from Dako (Carpinteria, CA); polyclonal anti-S-100 antiserum was from Milab (Malmo, Sweden); the polyclonal anti-epidermal keratin antiserum was produced in this laboratory. The preparation and characterization of anti-epidermal keratin antiserum have been previously described (Kameda et al, 1987(Kameda et al, ,1986. Preliminary trypsinization was done for the immunoreaction of antiepidermal keratin antiserum (Kameda et al, 1986).…”

Cellular and molecular events on the development of mammalian thyroid C cells

“…These complexes usually consist of C cells at various stages of development and cystic structures of various sizes. The cysts immunostain with antiserum against 50,000‐ and 58,000‐dalton keratins extracted from canine foot‐sole epidermis (Kameda, ). The complexes are considered to represent persistent ultimobranchial bodies that have not fused, or that have fused only partially, with the thyroid.…”