Differential distribution of S-100 protein and vimentin in the hypophyseal pars tuberalis of the guinea pig.

Kameda, Yoko

doi:10.1177/44.5.8627006

Cited by 14 publications

(10 citation statements)

References 32 publications

(42 reference statements)

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“…In the preceding study of the guinea pig pars tuberalis, I found that a large number of vimentin-immunoreactive cells were distributed in the region surrounding the median eminence and in the dorsocaudal region adjacent to the infundibular stalk (Kameda, 1996). Conversely, S-100-immunoreactive cells were concentrated in the ventrocaudal region of continuity with the pars distalis.…”

Section: Introductionmentioning

confidence: 93%

Ultrastructural immunogold localization of vimentin and S-100 protein in guinea pig pars tuberalis.

Kameda

1996

J Histochem Cytochem.

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Ultrastructural features of vimentin- and S-100 protein-positive cells in guinea pig pars tuberalis were examined by immunoelectron microscopy with the postembedding immunogold method. Interstitial cells that exhibited elongated cytoplasm and partly surrounded the specific secretory cells were filled with intermediate filament bundles on which immunogold particles for vimentin were densely located. Small colloid-containing follicles were occasionally distributed in the cranial region surrounding the median eminence. The follicular cells lining the luminal cavities contained considerable amounts of intermediate filaments immunoreactive for vimentin. Some specific secretory cells displayed various amounts of intermediate filaments immunoreactive for vimentin, which were scattered throughout the cytoplasm or concentrated around the nucleus. The specific secretory cells in which many parallel profiles of rough endoplasmic reticulum (RER) or a large number of mitochondria were dispersed throughout cytoplasm, i.e., being highly metabolically active, were devoid of intermediate filaments. The ventrocaudal region in continuity with the pars distalis was occupied by cells that were packed with vesicular inclusions, considered to be dilated cisternae of RER, and frequently formed colloid-containing follicles. Immunoreactivity for S-100 protein was located exclusively in this novel cell type. Immunogold particles for S-100 protein were distributed in the cytoplasmic matrix and were also associated with the membrane of vesicular inclusions. Gold particles were also detected on the tight junctions, desmosomes, and fibril materials at the apical cell regions of the colloid-containing follicles. The finding of two separate populations of cells containing vimentin and S-100 protein, respectively, supports the idea of functional separation in the pars tuberalis.

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Section: Introductionmentioning

confidence: 93%

Ultrastructural immunogold localization of vimentin and S-100 protein in guinea pig pars tuberalis.

Kameda

1996

J Histochem Cytochem.

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“…A majority of the pars tuberalis, however, consists of secretory cells specific to this portion of the pituitary and which do not resemble any cell type of the pars distalis. In addition, there are follicular cells and interstitial cells which correspond to the folliculo-stellate cells of the pars distalis (Kameda 1990(Kameda , 1996. The secretory-specific cells of the pars tuberalis of mammalian species usually contain only a few small secretory granules about 100 nm in diameter (Dellmann et al 1974).…”

Section: Introductionmentioning

confidence: 98%

Localization and development of chromogranin A and luteinizing hormone immunoreactivities in the secretory-specific cells of the hypophyseal pars tuberalis of the chicken

Kameda

Miura

Ohno

1998

Histochemistry and Cell Biology

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The pars tuberalis mainly consists of the secretory cells specific to this portion of the pituitary. We examined the localization and development of luteinizing hormone (LH) and chromogranin A in the chicken pars tuberalis by immunohistochemistry. The vast majority of the chicken pars tuberalis was occupied by cells immunoreactive for both LH and chromogranin A. Furthermore, immunoblot analysis of chicken pars tuberalis extracts with LH antiserum demonstrated that two bands, the large alpha-subunit and small beta-subunit of the LH molecule, were expressed in this tissue as well as in the pars distalis. A band for chromogranin A was also detected in pars tuberalis extracts with chromogranin A antiserum. In contrast to the cells of mammalian species that contain only a few small secretory granules, the specific cells of the chicken pars tuberalis were characterized by the presence of many secretory granules ranging from 90 to 400 nm in diameter. Postembedding immunogold labeling showed that gold particles representing immunoreactivity for LH were densely located on all secretory granules of the secretory-specific cells. Many secretory granules, especially the large ones, of the cells were also loaded with immunogold particles for chromogranin A. Double immunogold labeling confirmed that LH and chromogranin A were colocalized on the same secretory granules. During embryonic development, the primordium of the pars tuberalis was first detected at 8 days of incubation as a small group of cells containing LH- and chromogranin-immunoreactive cells. In the pars distalis, the onset of LH and chromogranin expression occurred earlier, at 6 days of incubation. At 10 days of incubation, the pars tuberalis primordium became large cell masses consisting of LH- and chromogranin-immunoreactive cells, which were located close to the median eminence. Subsequently, the primordium extended along the median eminence progressively with age. At 14 days of incubation, it reached to the rostral end and surrounded the median eminence as slender cell cords. These results indicate that specific cells of the chicken pars tuberalis synthesize a glycoprotein hormone related to the LH molecule, which is stored in the secretory granules together with chromogranin A. The pars tuberalis may be involved in the regulation of gonadal function in a different way from that of the pars distalis.

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“…There are two types of cells in the pars tuberalis, i.e., specific secretory cells (PT cells), and follicular and interstitial cells corresponding to folliculo-stellate cells in the pars distalis (Wittkowski et al 1999;Kameda 1996a). The PT cells possess small secretory granules which are not similar to any of the other well-known cell types of the adenohypophysis (Dellmann et al 1974).…”

Section: Introductionmentioning

confidence: 99%

Hes1 regulates formations of the hypophyseal pars tuberalis and the hypothalamus

et al. 2010

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The hypophyseal pars tuberalis surrounds the median eminence and infundibular stalk of the hypothalamus as thin layers of cells. The pars tuberalis expresses MT1 melatonin receptor and participates in mediating the photoperiodic secretion of pituitary hormones. Both the rostral tip of Rathke's pouch (pars tuberalis primordium) and the pars tuberalis expressed alphaGSU mRNA, and were immunoreactive for LH, chromogranin A, and TSHbeta in mice. Hes genes control progenitor cell differentiation in many embryonic tissues and play a crucial role for neurulation in the central nervous system. We investigated the Hes1 function in outgrowth and differentiation of the pars tuberalis by using the markers for the pars tuberalis. In homozygous Hes1 null mutant embryos, the rostral tip was formed in the basal-ventral part of Rathke's pouch at embryonic day (E)11.5 as well as in wild-type embryos. In contrast to the wild-type, the rostral tip of null mutants could not extend rostrally with age; it remained in the low extremity of Rathke's pouch during E12.5-E13.5 and disappeared at E14.5, resulting in lack of the pars tuberalis. Development of the ventral diencephalon was impaired in the null mutants at early stages. Rathke's pouch, therefore, could not link with the nervous tissue and failed to receive inductive signals from the diencephalon. In a very few mutant mice in which the ventral diencephalon was partially sustained, some pars tuberalis cells were distributed around the hypoplastic infundibulum. Thus, Hes1 is required for development of the pars tuberalis and its growth is dependent on the ventral diencephalon.

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Differential distribution of S-100 protein and vimentin in the hypophyseal pars tuberalis of the guinea pig.

Cited by 14 publications

References 32 publications

Ultrastructural immunogold localization of vimentin and S-100 protein in guinea pig pars tuberalis.

Ultrastructural immunogold localization of vimentin and S-100 protein in guinea pig pars tuberalis.

Localization and development of chromogranin A and luteinizing hormone immunoreactivities in the secretory-specific cells of the hypophyseal pars tuberalis of the chicken

Hes1 regulates formations of the hypophyseal pars tuberalis and the hypothalamus

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