Co-expression of vimentin and 19S-thyroglobulin in follicular cells located in the C-cell complex of dog thyroid gland.

The purpose of our publication is to widely communicate the pictures of spontaneous findings occurring in beagles. Spontaneous arteritis occurs commonly in beagles. Frequent sites of arteritis are the heart, spleen, pancreas, epididymis and spinal cord. Morphological similarities between spontaneous and drug-induced arterial lesions may cause confusion when evaluating vascular toxicity of chemicals such as vasodilating agents. Focal and minimal inflammatory lesions are occasionally seen in the lung and may be associated with aspiration of food particles or of unknown causes. A cystic change with copious mucin production occurs occasionally in the mucosal epithelium of the gall bladder. Nesidioblastosis is seen rarely in the pancreas of beagles. C-cell complex and lymphocytic thyroiditis are common thyroid lesions. Spontaneous focal hypospermatogenesis and lobular Sertoli-cell-only seminiferous tubules occurring frequently in beagles must be distinguished from drug-induced damage of the seminiferous tubules in toxicity studies. The morphological differences of the female genital system in each cycle need to be understood; therefore, we present the normal features of the cyclic changes of the female genital organs. Further, we provide more information on spontaneous findings in beagles for exact diagnoses in toxicity studies.

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“…76) 26 , C-cell complex (Fig. 124) 27 , lymphocytic thyroiditis (Fig. 130) 28 and Renaut bodies in the sciatic nerve (Fig.…”

Section: Discussionmentioning

confidence: 99%

Histopathology of Incidental Findings in Beagles Used in Toxicity Studies

et al. 2012

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“…Vimentin is an intermediate filament protein initially described in mesenchymal derivatives. It is co-located in immature cells with other kinds of intermediate filaments at particular stages of development or differentiation and is transiently expressed in various cell types during embryonic development (see [30] for references). Additionally, the clone V9 was recently shown to express in tilapia differentiating cells [31].…”

Section: Methodsmentioning

confidence: 99%

An immunohistochemical study of gill epithelium cells in the Nile tilapia, Oreochromis niloticus.

Monteiro

Fontaínhas‐Fernandes²,

Sousa³

2010

Folia Histochem Cytobiol.

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This study reports the first complete mapping of the gill epithelium in a tilapia species. Different gill epithelial cell types of the Nile tilapia, Oreochromis niloticus L. have been identified and located using different antisera against mammalian proteins and various histochemical techniques: Periodic Acid Schiff (PAS), Alcian Blue pH 1.0, 2.5, 3.5, Giemsa and Grimelius. The results show that the stratified filament epithelium of O. niloticus gill can be divided into two distinct regions, a superficial layer, where pavement, mucous and mitochondria rich cells can be found, and a deep layer, constituted by undifferentiated, myoepithelial-like, granular and neuroendocrine cells. V-ATPase and Na + /K + -ATPase presence allowed the identification of pavement and mitochondria-rich cells, respectively, suggesting that, in O. niloticus, pavement cells are implicated in Na + uptake, whereas mitochondria-rich cells have a role in Cl -uptake. The use of PAS and Alcian Blue allowed the recognition of different sub-populations of mucous cells that differentiate from a common deeper precursor. Neuroendocrine markers were detected in different cell populations, stating evidence for a neuroendocrine role of mitochondria-rich cells, and suggesting the existence of distinct neural pathways, a putative O 2 -chemosensory and an ion regulatory pathway. A defence role was attributed to the deep filament epithelium, suggested by the presence of resident giemsa positive-eosinophil granular cells. The antibody raised against proliferating cell nuclear antigen identified two different cell types, the undifferentiated cells and myoepithelial-like cells. In the superficial layer, it is here stated for the first time the existence of vimentin positive support cells.

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“…The ultimobranchial body origin of solid cell nests was first proposed by Erdheim in 1904, 24 and marked similarities were pointed out between the solid cell nests and the ultimobranchial body of the rat, 25 dog, 26 and bull. 27 The unusual association of solid cell nests with thymic, [28][29][30][31] parathyroid, 28,29 adipose, 1,30 fibrous, 32 cartilaginous, 1,32 and salivary gland-type tissues 32 has been documented, and together with the production of mucins 1,28,33,34 reinforces the endodermal, ultimobranchial body origin of solid cell nests.…”

Section: Discussionmentioning

confidence: 99%

Telomerase expression and proliferative activity suggest a stem cell role for thyroid solid cell nests

et al. 2004

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Solid cell nests of the human thyroid gland are composed of main cells and C cells. In order to investigate the putative stem cell nature of the role for solid cell nests, we evaluated the histological features, and the immunohistochemical expression of p63, bcl-2, telomerase catalytic subunit, and two proliferative markers (Ki-67 and minichromosome maintenance protein 2), in a series of 24 cases of solid cell nests. Proliferative indices were determined in (a) solid cell nests, (b) thyroid follicular cells in the vicinity of solid cell nests within a low-power field, and (c) distant thyroid tissue, at a distance of at least three low-power fields from solid cell nests. In 15 cases of solid cell nests (62.5%), mixed follicles were observed; papillary formations were observed in four cases (16.6%), and ciliated cells were observed in the lining of microcysts associated with two cases (8.3%). Salivary gland-type tissue, cartilage islands, adipose and fibrous tissues, and small nerves were also associated with some cases of solid cell nests. We observed that the main cells of the solid cell nests express consistently telomerase, although at lower levels than p63, and show strong cytoplasmic immunoreactivity for bcl-2, which is associated with an increased differentiation potential. We also observed that despite their relative low proliferative index, main cells of the solid cell nests display higher proliferation than follicular cells in the vicinity and follicular cells in more distant thyroid tissue. We conclude that main cells of the solid cell nests apparently harbor the minimal properties of a stem cell phenotype (capacity for both self-renewal, conferred by telomerase activity, and differentiation to one or more than one type of specialized cells, given by the high expression of p63 and bcl-2) and may thus represent a pool of stem cells of the adult thyroid.

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Co-expression of vimentin and 19S-thyroglobulin in follicular cells located in the C-cell complex of dog thyroid gland.

Cited by 20 publications

References 19 publications

Histopathology of Incidental Findings in Beagles Used in Toxicity Studies

Histopathology of Incidental Findings in Beagles Used in Toxicity Studies

An immunohistochemical study of gill epithelium cells in the Nile tilapia, Oreochromis niloticus.

Telomerase expression and proliferative activity suggest a stem cell role for thyroid solid cell nests

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