Immunogold–silver staining-on-a-chip biosensor based on cross-flow chromatography

Cho, Il‐Hoon; Seo, Sung Chul; Paek, Eui-Hwan; Paek, Se‐Hwan

doi:10.1016/j.jchromb.2009.07.016

Cited by 52 publications

(39 citation statements)

References 28 publications

(47 reference statements)

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“…For example, photothermal heating of the NPs [35] can be used to increase the signal of the test line, where a laser is used to excite the gold NPs at their SPR and increase image contrast by thermal detection. Signal enhancement has been achieved by enzymes such as horseradish peroxidase (HRP) [36], silver staining, [37] or even the use of multiple gold NPs binding species [38], all of which increase assay sensitivity. Induced NP aggregation has also been used to increase the signal or effect a color change.…”

Section: Towards Improved Nanotechnology-enabled Lfasmentioning

confidence: 99%

Challenges of the Nano–Bio Interface in Lateral Flow and Dipstick Immunoassays

Puig

Bosch²,

Gehrke

et al. 2017

Trends in Biotechnology

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Lateral flow assays (LFAs) are highly attractive for point of care diagnostics for infectious disease, food safety, and many other medical uses. The unique optical, electronic and chemical properties that arise from the nanostructured and material characteristics of nanoparticles provide an opportunity to increase LFA sensitivity and impart novel capabilities. However, interfacing to nanomaterials in complex biological environments is challenging and can result in undesirable side effects such as non-specific adsorption, protein denaturation, and steric hindrance. These issues are even more acute in LFAs, where there are many different types of inorganic-biological interfaces, often of complex nature. Therefore, the unique properties of nanomaterials for LFAs must be exploited in a way that addresses these interface challenges.

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Section: Towards Improved Nanotechnology-enabled Lfasmentioning

confidence: 99%

Challenges of the Nano–Bio Interface in Lateral Flow and Dipstick Immunoassays

Puig

Bosch²,

Gehrke

et al. 2017

Trends in Biotechnology

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show abstract

“…[1][2][3] Although the assays provide signals that are visible with the naked eye, further signal amplification can enhance the detection sensitivity. [4][5][6] However, such amplification process usually requires additional steps for the delivery of multiple reagents in a sequential manner for incubation and washing steps, which makes it difficult to design a device that can perform sequential delivery of real samples with a simple onestep procedure.…”

Section: Introductionmentioning

confidence: 99%

Programmed sample delivery on a pressurized paper

et al. 2014

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This paper reports a method to control the fluid flow in paper-based microfluidic devices simply by pressing over the channel surface of paper, thereby decreasing the pore size and permeability of a non-woven polypropylene sheet. As a result, fluid resistance is increased in the pressed region and causes flow rate to decrease. We characterize the decrease of flow rate with respect to different amounts of pressure applied, and up to 740% decrease in flow velocity was achieved. In addition, we demonstrate flow rate control in a Y-shaped merging paper and sequential delivery of multiple color dyes in a three-branched paper. Furthermore, sequential delivery of multiple fluid samples is performed to demonstrate its application in multi-step colorimetric immunoassay, which shows a 4.3-fold signal increase via enhancement step. V C 2014 AIP Publishing LLC.

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“…The Paek group made use of this method to test for the model analyte cardiactroponin I (cTnI), a specific biomarker for acute myocardial infarction. 39 The Paek group determined that gold nanoparticles with a 5-nm diameter were optimal for IGSS, as it had the shortest reaction time with the silver solution. A cross-flow system was employed, which allowed the analyte to flow vertically and the silver-enhancing solution to flow horizontally on the LFA strip.…”

Section: Signal Amplificationmentioning

confidence: 99%

Paper-Based Systems for Point-of-Care Biosensing

2015

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Paper-based systems have been widely investigated for developing point-of-care devices because of their simplicity, affordability, and ease of use. Recent advances have resulted in paper systems that have progressed beyond the historical "single-strip" format and allow for a larger range of functions. This review provides a summary of the advances that have been made to improve the utility of paper-based diagnostic tests for biosensing. Specifically, techniques for designing paper devices, including different geometries and chemical patterning to control fluid flow, are discussed. This review also examines novel approaches to improve paper-based assay sensitivities, such as sample preconcentration, signal amplification at the detection zone, and electrochemical methods.

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Immunogold–silver staining-on-a-chip biosensor based on cross-flow chromatography

Cited by 52 publications

References 28 publications

Challenges of the Nano–Bio Interface in Lateral Flow and Dipstick Immunoassays

Challenges of the Nano–Bio Interface in Lateral Flow and Dipstick Immunoassays

Programmed sample delivery on a pressurized paper

Paper-Based Systems for Point-of-Care Biosensing

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