Immunoglobulins on the surface of lymphocytes

Grey, Howard M.; Rabellino, Enrique M.; Pirofsky, Bernard

doi:10.1172/jci106735

Cited by 374 publications

(17 citation statements)

References 14 publications

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“…Fur-thermore, in freeze-thaw lysates containing the total surface and cytoplasmic immunoglobulin, it was demonstrated that similar amounts of immunoglobulin were associated with the cells that lacked detectable SIg by immunofluorescence, as compared to those which contained it. Also, these amounts of immunoglobulin are only slightly less than those found in normal B cells and are much larger than any amount of immunoglobulin which has been previously (letecte(l in T cell populations (26,27).…”

Section: Discussionmentioning

confidence: 62%

Combined Studies of Complement Receptor and Surface Immunoglobulin-Bearing Cells and Sheep Erythrocyte Rosette-Forming Cells in Normal and Leukemic Human Lymphocytes

Ross¹,

Rabellino²,

Polley³

et al. 1973

J. Clin. Invest.

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364

157

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A B S T R A C T Human lymphocytes from normal peripheral blood, thymus, spleen, thoracic duct, and peripheral lymphocytes from patients with chronic lymphatic leukemia were studied for complement receptor sites (CRL), surface immunoglobulin (SIg), and for the ability to form rosettes with sheep erythrocytes (TRFC). The two B cell markers (CRL and SIg) were found to be in overlapping, but not totally identical populations, whereas cells that were able to form rosettes were found in a totally unrelated population of lymphocytes; TRFC is therefore probably a reliable marker for T cells. In peripheral blood 24% of lymphocytes had SIg, but only half of these were also CRL. Almost all of the non-SIg peripheral blood lymphocytes were TRFC. In the spleen and thoracic duct only a few lymphocytes were observed that had SIg and were not CRL. On the other hand, in two of three spleens studied 10-20% of cells were CRL that did not have SIg. In the thoracic duct all non-CRL, non-SIg cells were TRFC. In chronic lymphatic leukemia three findings were made: (a) The presence or absence of CRL was independent of the presence or absence of SIg so that in individuals whose cells were non-SIg, CRL were usually plentiful.

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Section: Discussionmentioning

confidence: 62%

Combined Studies of Complement Receptor and Surface Immunoglobulin-Bearing Cells and Sheep Erythrocyte Rosette-Forming Cells in Normal and Leukemic Human Lymphocytes

Ross¹,

Rabellino²,

Polley³

et al. 1973

J. Clin. Invest.

Self Cite

364

157

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“…For identification of B-cells the immunofluorescent technique of Grey et al 3 was used with a slight modification. Fifty PI of mononuclear cell suspension at a concentration of 2 X 106/ml were incubated with 50 PI of fluorescein conjugated goat anti-human immunoglobulin (polyvalent) (Behring Diagnostics, Somerville, N.J.) for one hour at room temperature, shaking gently every 10 minutes.…”

Section: Cancer May 1977mentioning

confidence: 99%

Lymphoblasts with T-cell markers in five girls with acute lymphocytic leukemia

Hann

London

Evans

1977

Cancer

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In previous reports, children with acute lymphoblastic leukemia (ALL), who presented with T-cell markers on their lymphoblasts (T-lymphoblasts), have been predominantly older boys often with a mediastinal mass. These children are thought to constitute a subgroup of childhood ALL that has the poorest prognosis. Here, we report five girls with ALL, who presented with T-lymphoblasts but without a mediastinal mass. All responded well to chemotherapy and three of five are in maintained remission 21-33 months after diagnosis. The fourth patient died while in remission of causes other than leukemia and the fifth relapsed and died of infection two years after diagnosis. These observations suggest that girls with T-lymphoblasts may not have an adverse prognosis.

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“…Most cases of chronic lymphocytic leukaemia (CLL) and non-Hodgkin's lymphoma (NHL) represent a proliferation of neoplastic B lymphocytes (Grey et al, 1971). Such cells are not generally regarded as secreting cells, and the majority of immunoglobulin (Ig) synthesized is for insertion into the plasma membrane (Stevenson, 1976).…”

mentioning

confidence: 99%