Monoclonal immunoglobulin light chain in urine of patients with B lymphocytic disease: Its source and use as a diagnostic aid

Stevenson, Freda K.; Spellerberg, Myfanwy B.; Smith, Joan K.

doi:10.1038/bjc.1983.98

Cited by 18 publications

(8 citation statements)

References 10 publications

(9 reference statements)

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“…Investigation of different B-cell malignancies has shown that BJP could be detected in at least one half of the cases. This is in accordance with previous work using this (Abelev et al, 1983;Abelev and Karamova, 1987) or other techniques (Pierson et al, 1980;Stevenson et al, 1983;Pascali and Pezzoli, 1986). This suggests that BJP is a valuable tumor marker not only for multiple myeloma but also for other kinds of B-cell malignancy.…”

Section: Discussionsupporting

confidence: 92%

“…These results were in line with earlier data of Pierson et a1. (1980) who used, however, a much more complicated method for detecting trace amounts of BJP in CLL.The presence of BJP in a significant proportion of CLL and NHL patients has also been observed using a combination of means to concentrate urine associated with: electrophoresisimmunofixaltion (Pascali and Pezzoli, 1986;Pezzoli and PasCali, 1986):, immunoisoelectrofocusing (Stevenson et al, 1983) or iso'electrofocusing in agarose gels coupled with immunoblotting (Norden et al, 1987).BJP may thus serve as an immunological marker not only in multiple myeloma, where it is quite common, but also in CLL and NHL, where it has commonly been considered to be very rare (Bergsagel and Pruzansky, 1979).However, BJP has not been used to follow up CLL or NHL patients in t:he course of chemotherapy, particularly in longterm monitoring of patients undergoing several episodes of remission and relapse. In particular, patients with complete clinical remission have not been systematically studied in this respect .…”

mentioning

confidence: 95%

“…The presence of BJP in a significant proportion of CLL and NHL patients has also been observed using a combination of means to concentrate urine associated with: electrophoresisimmunofixaltion (Pascali and Pezzoli and Pas-Cali, 1986):, immunoisoelectrofocusing (Stevenson et al, 1983) or iso'electrofocusing in agarose gels coupled with immunoblotting (Norden et al, 1987).…”

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confidence: 98%

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Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease

Абелев

Karamova

Bragina³

et al. 1990

Intl Journal of Cancer

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We suggest that countercurrent isotachophoresis performed on cellulose acetate membranes (ITP-CAM) should be used for detecting trace amounts of Bence-Jones protein (BJP) in urine of patients with chronic lymphocytic leukemia (CLL), non-Hodgkin's lymphoma (NHL) and related diseases. ITP-CAM allows simultaneous concentration and electrophoretic separation of proteins present in highly diluted solutions, as well as easy immunological detection of separated substances. BJP was found in 24 out of 42 patients with CLL, 33 of 56 with NHL and 3 of 3 with Waldenström macroglobulinemia. Twenty-three patients were followed during the course of chemo- or radiotherapy. In 19 cases the BJP findings correlated well with clinical status. In no case of partial or complete clinical response did BJP completely disappear from the urine.

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Section: Discussionsupporting

confidence: 92%

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confidence: 95%

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confidence: 98%

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Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease

Абелев

Karamova

Bragina³

et al. 1990

Intl Journal of Cancer

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“…Three of them had NHL, three had CLL, and two had MGUS. All these patients had a polyclonal increase in immunoglobulin concentration, no homogeneous immunoglobulin bands being detectable by No. 11 PURE BENCE JONES PROTEINURIA .…”

Section: Resultsmentioning

confidence: 93%

The clinical spectrum of pure bence jones proteinuria: A study of 66 patients

Pascali

Pezzoli

1988

Cancer

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Sixty-six consecutive patients exhibiting isolated urinary excretion of monoclonal free light chains, i.e. Bence Jones protein (BJP), on screening investigation for serum and urine monoclonal immunoglobulins were studied in order to better define the spectrum of immunoproliferative disorders associated with such a protein abnormality. The typical plasma cell neoplasms accounted for only one third of the cases, multiple myeloma (MM) and systemic amyloidosis (AL) being diagnosed in 18% and 15% of the patients, respectively. Eighteen (27%) of the patients were recognized as having malignant nonHodgkin's lymphomas (NHL), 21 (32%) had chronic lymphocytic leukemia (CLL), and 2 (3%) had hairy cell leukemia (HCL). Three patients (5%) without apparent evidence of any malignant immunoproliferative disease were classified as having a monoclonal gammopathy of undetermined significance (MGUS). The greatest urinary concentrations of BJP were found in plasmacytic neoplasms, the daily excretion of MM patients being significantly higher than that of AL patients. Considerably lower BJP outputs were recorded in the other diseases, the lowest ones being associated with MGUS. NHL patients had a daily excretion four times higher as compared with that of CLL patients. The distribution of NHL by histologic type was: follicular center cell lymphomas (FCCL) 39%, small lymphocytic lymphoma (SLL) 33%, immunoblastic lymphoma (IBL) 17%, and plasmacytoid lymphocytic lymphoma (PLL) 11%. The highest BJP levels were found in PLL, and the lowest ones in FCCL. In CLL patients the amount of urinary BJP correlated significantly with the tumor load, as estimated by the number of enlarged lymphoid areas. The study suggests that detection and measurement of isolated urinary BJP may provide useful data for the clinical evaluation of a wide spectrum of immunoproliferative disorders.

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“…By means of recently introduced methods with more sensitivity, however, the accumulation of monoclonal light-chain proteins could be demonstrated in 40%-65% of the NHL cases (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Monoclonal free light chains in urine and their significance in clinical diagnostics: Are they really tumor markers?

Végh

Ottó

Eckhardt

1990

Clinical Laboratory Analysis

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Bence Jones proteins (monoclonal free light chains of immunoglobulins) are the earliest known biological markers of malignant cell dyscrasia; Bence Jones proteinuria is also present in many types of B cell-related neoplasms. Sometimes, it may also occur in Hodgkin's disease. In some cases, benign monoclonal gammapathy was found to be associated nontumorous diseases as well. The type of monoclonal light chain, the degree of polymerization, and the isoelectric point of the molecule may affect the course of the disease. Urine samples from 637 patients with true or suspected lymphoproliferative diseases were investigated over a 2-yr period by different immunochemical methods. Bence Jones proteinuria was identified in 71 cases by isoelectric focusing combined with immunofixation, while the pathological protein was detected only in 63 cases by conventional methods. Bence Jones proteins can be detected by this new method at a level below the sensitivity of conventional procedures. Bence Jones proteins in the urine may signal a malignant tumor or malignant transformation of an earlier disease. The early detection of monoclonal immunoglobulin light chains in the urine may be important in clinical diagnosis, therapy, and follow-up.

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Monoclonal immunoglobulin light chain in urine of patients with B lymphocytic disease: Its source and use as a diagnostic aid

Cited by 18 publications

References 10 publications

Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease

Immuno‐isotachophoretic determination of monoclonal immunoglobulin light chains produced by neoplastic b‐cells: Use in diagnosis, monitoring and detection of residual disease

The clinical spectrum of pure bence jones proteinuria: A study of 66 patients

Monoclonal free light chains in urine and their significance in clinical diagnostics: Are they really tumor markers?

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