Immunoglobulin in fish—genes, expression and structure

Pilström, Lars; Bengtén, Eva

doi:10.1006/fsim.1996.0026

Cited by 79 publications

(48 citation statements)

References 70 publications

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“…1, left, lane 2), most likely IgM subunits as reported from other fish species (40), and the observation from SEC and nonreducing SDS-PAGE of a large molecular weight species (Ͼ500 kDa) that resolves into 80-and 27-kDa bands upon reduction (data not shown). After the second purification step (i.e.…”

Section: Purification and Biochemical Characterization Of Msafbp32-asupporting

confidence: 58%

Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis)

Odom

Vasta

2006

Journal of Biological Chemistry

137

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Among other functions, lectins play an important role in the innate immune response of vertebrates and invertebrates by recognizing exposed glycans on the surface of potential pathogens. Despite the typically weak interaction of lectin domains with their carbohydrate ligands, they usually achieve high avidity through oligomeric structures or by the presence of tandem carbohydratebinding domains along the polypeptide. The recently described structure of the fucose-binding European eel agglutinin revealed a novel lectin fold (the "F-type" fold), which is shared with other carbohydrate-binding proteins and apparently unrelated proteins from prokaryotes to vertebrates, and a unique fucose-binding sequence motif. Here we described the biochemical and molecular characterization of a unique fucose-binding lectin (MsaFBP32) isolated from serum of the striped bass (Morone saxatilis), composed of two tandem domains that exhibit the eel carbohydrate recognition sequence motif, which we designate F-type. We also described a novel lectin family ("F-type") constituted by a large number of proteins exhibiting greater multiples of the F-type motif, either tandemly arrayed or in mosaic combinations with other domains, including a putative transmembrane receptor, that suggests an extensive functional diversification of this lectin family. Among the tandem lectins, MsaFBP32 and other tandem binary homologues appear unique in that although their N-terminal domain shows close similarity to the fucose recognition domain of the eel agglutinin, their C-terminal domain exhibits changes that potentially could confer a distinct specificity for fucosylated ligands. In contrast with the amniotes, in which the F-type lectins appear conspicuously absent, the widespread gene duplication in the teleost fish suggests these F-type lectins acquired increasing evolutionary value within this taxon.

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Section: Purification and Biochemical Characterization Of Msafbp32-asupporting

confidence: 58%

Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis)

Odom

Vasta

2006

Journal of Biological Chemistry

137

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“…The presence of a single band was also indication of the existence of only a major type of antibody in Asian sea bass. In majority of the osteichthyan species, a single type of antibody has been reported [16,17]. In the present study, sea bass Ig was found to possess a molecular weight of 896 kDa.…”

Section: Discussionsupporting

confidence: 54%

“…Immunoglobulin had been isolated from a variety of fish species such as European eel [1], channel catfish [2], pike [3], Atlantic salmon [4], giant grouper [5], European sea bass [6], European perch [7], sea bream [8,9], tilapia [10], turbot [11], Japanese eel [12], Indian major carp [13], and Asiatic cat fish [14]. Many previous studies had shown that most teleost fish immunoglobulin were structurally similar to mammalian immunoglobulin M (IgM) [15,16]. Molecular weight of fish IgM was found, ranging from 608-900 kDa with a tetrameric configuration [17].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of immunoglobulin M of Asian sea bass, Lates calcarifer and its level in serum

Choudhury

Prasad

2011

Open Life Sciences

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Asian sea bass immunoglobulin M (IgM) was purified from the sera of Lates calcarifer by affinity chromatography. Analysis of the purified IgM on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions revealed that the sea bass IgM was a tetrameric protein with a molecular weight of 896 kDa; it contained an equimolar heavy chain and light chain with molecular weight of 83 kDa and 27 kDa respectively. However, besides the covalently linked tetrameric IgM, noncovalently linked tetramer dissociated into dimeric and monomeric forms also demonstrated by non-reducing SDS-PAGE. Carbohydrate moieties were found to be linked with both heavy and light chains. A polyclonal rabbit anti-Asian sea bass IgM was prepared which showed a specific reaction of anti-fish IgM antibody with IgM of sea bass. Sea bass IgM concentration was determined in the serum by indirect ELISA. The average IgM concentration in the sera of the healthy sea bass was 5.4±1.8 mg ml−1; it amounted to 16.7% of the total serum protein.

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“…A lisozima é uma molécula de defesa importante do sistema imune inata, que pode desempenhar um importante papel no peixe como um mecanismo de defesa contra doenças infecciosas (SAURABH e SAHOO, 2008;LIE et al, 1989). As imunoglobulinas (Ig) que constituem os anticorpos são utilizadas na defesa imunológica contra agentes patogênicos em todos os vertebrados (PILSTRÖM; BENGTÉN, 1996).…”

Section: Resposta Imuneunclassified

Selenium and Vitamin in Diets for Tilapia-Do-Nilo

Botelho

Leira²,

Melo³

et al. 2017

Nucleus

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RESUMO:Dentre as espécies de peixes que demonstram potencial para o cultivo no Brasil, a tilápia-do-Nilo (Oreochromis niloticus) é considerada a espécie mais produtiva. Essa espécie apresenta diversas vantagens, entre elas: rápido crescimento, maior rendimento de filé, resistência a baixa qualidade de água e boa aceitação no mercado consumidor. Na nutrição de peixes vários são os aspectos a serem avaliados em uma ração. O selênio e a vitamina E fazem parte de um sistema de defesa antioxidante multicomponente. Este sistema protege as células contra os efeitos adversos das espécies reativas de oxigênio e outros radicais livres que iniciam a oxidação. Além disso a suplementação de Vitamina E na dieta proporciona um maior desempenho produtivo, efeitos positivos nos parâmetros imunológicos dos peixes e resistência ao estresse. O selênio é um elemento que possui margem estreita entre os níveis de exigência e toxidez. As exigências são baixas, mas se não atingida, podem comprometer o sistema antioxidante e causar graves consequências para o metabolismo do animal. A capacidade do peixe em acumular selênio nos tecidos pode tornar a carne de pescado um interessante alimento funcional para a nutrição humana, especialmente como boa fonte de selênio. A tilápia-do-Nilo tem um nível de exigência específica de selênio e vitamina E que não pode ser satisfeito somente pelo ambiente aquático. Desta forma uma correta suplementação na dieta de minerais e vitaminas poderá garantir um maior desempenho, proporcionando uma melhor qualidade de carne e melhor aceitação no mercado.Palavras-chave: Antioxidante. Aquicultura. Imunológicos SELENIUM AND VITAMIN IN DIETS FOR TILAPIA-DO-NILO SUMMARY:Among the species of fish that demonstrate potential for cultivation in Brazil, Nile tilapia (Oreochromis niloticus) is considered the most productive species. This species has several advantages, including: rapid growth, increased fillet yield, low quality water resistance and good acceptance in the consumer market. In fish nutrition there are several aspects to be evaluated in a ration. Selenium and Vitamin E are part of a multicomponent antioxidant defense system. This system protects the cells against the adverse effects of reactive oxygen species and other free radicals that initiate oxidation. In addition, supplementation of Vitamin E in the diet provides greater productive performance, positive effects on immunological parameters of fish and resistance to stress. Selenium is an element that has a narrow margin between the levels of demand and toxicity. The requirements are low, but if not achieved, could compromise the antioxidant system and cause serious consequences for the animal metabolism. The fish's ability to accumulate selenium in tissues can make fish fillet an interesting functional food for human nutrition, especially as a good source of selenium. Nile Tilapia has a level of specific requirement of selenium and vitamin E that cannot be satisfied only by the aquatic environment. Thus a correct supplementation of minerals and vitamins ...

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Immunoglobulin in fish—genes, expression and structure

Cited by 79 publications

References 70 publications

Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis)

Characterization of a Binary Tandem Domain F-type Lectin from Striped Bass (Morone saxatilis)

Isolation and characterization of immunoglobulin M of Asian sea bass, Lates calcarifer and its level in serum

Selenium and Vitamin in Diets for Tilapia-Do-Nilo

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