Immunogenicity of recombinant vaccinia viruses expressing hepatitis B virus surface antigen in mice

Grigorieva, Irina M.; Grigoriev, Vitaly G.; Zakharova, L. G.; Pashvykina, Galina V.; Shevlyagin, V. Ya.; Altstein, Anatoly D.

doi:10.1016/0165-2478(93)90099-n

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…In agreement with some earlier reports (Morgan et al, 1988 ;Lee et al, 1992 ;Grigorieva et al, 1993 ;Kutinova! et al, 1996), the virulence of parental VV appeared to be the most important factor that influenced the result of immunization.…”

Section: Discussionsupporting

confidence: 93%

“…In agreement with some earlier reports (Morgan et al , 1988 ; Lee et al , 1992 ; Grigorieva et al , 1993 ; Kutinová et al , 1996 ), the virulence of parental VV appeared to be the most important factor that influenced the result of immunization. The varying immunogenicity of recombinants that differed in residual virulence could be due to different rates of replication in vivo .…”

Section: Discussionsupporting

confidence: 91%

“…reported that the immunogenicity of recombinants based on efficiently replicating VV is dependent on the virulence of the virus used for the recombinant preparation (Morgan et al, 1988 ;Lee et al, 1992 ;Grigorieva et al, 1993 ;Kutinova! et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, there are several questions associated with the preparation of safe and sufficiently immunogenic VV recombinants that will have to be answered first. It has been reported that the immunogenicity of recombinants based on efficiently replicating VV is dependent on the virulence of the virus used for the recombinant preparation (Morgan et al , 1988 ; Lee et al , 1992 ; Grigorieva et al , 1993 ; Kutinov á et al , 1996 ). However, some proteins can apparently be sufficiently immunogenic even when presented by highly attenuated (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Effect of virulence on immunogenicity of single and double vaccinia virus recombinants expressing differently immunogenic antigens: antibody-response inhibition induced by immunization with a mixture of recombinants differing in virulence

Kutinovà

Ludvı́ková

Marešová

et al. 1999

Journal of General Virology

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It has been shown recently that the residual virulence of vaccinia virus (VV) is an important factor that influences the outcome of immunization with VV recombinants. This study focused on the correlation of the residual virulence of several VV recombinants with antibody responses against the strongly immunogenic extrinsic glycoprotein E of varicella-zoster virus and the weakly immunogenic extrinsic protein preS2-S of hepatitis B virus and against VV proteins, with mice used as a model organism. Furthermore, the effects of mixing different recombinants on the antibody response were studied. The results obtained indicated that : (i) the antibody response depended on the residual virulence of the recombinants, more so in the case of the weakly immunogenic protein ; (ii) the residual virulence, the growth rate of the VV recombinants in extraneural tissues and the immunogenicity were associated features ; (iii) immunization with mixtures of two differently virulent recombinants or with unequal amounts of two similarly virulent recombinants sometimes led to the suppression of antibody response. The appearance of this suppression was dependent on three factors : the residual virulence of the recombinants, the immunogenicity of the extrinsic proteins and the ratio of the recombinants in the mixtures. Thus, the data obtained demonstrate that there are various limitations to the use of replicating VV recombinants for immunization purposes.

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Section: Discussionsupporting

confidence: 93%