Immunogenetic study on the polymorphism of serum ?2-lipoproteins in mink. II. Identification of allotypes Lpm-7 and Lpm-8 and genetic control of seven markers of the Lpm system

Abstract

By means of alloimmunization of mink, two new antigens, Lpm-7 and Lpm-8, were detected in their sera. Lpm-7 and Lpm-8 allospecificities were referred to a very high density alpha2-lipoprotein (Lpm) by the following criteria: histochemical tests, immunoelectrophoresis, preparative ultracentrifugation, and coalescence of alloprecipitates with heteroprecipitates in double diffusion tests. Genetic analysis indicated that Lpm-7 and Lpm-8, together with the earlier described Lpm-1, Lpm-2, Lpm-3, Lpm-4, and Lpm-5, share a common immunogenetic system. Polymorphism for the seven markers is conditioned by the genetic units Lpm8, Lpm4, Lpm4,8, Lpm4,7, Lpm3,4,8, Lpm1,8, Lpm1,2,7, and Lpm2,4,5,7, which behave as alleles. Of these units, the latter six are probably haploid sets of closely linked genes.

Selection for behavior and correlation with the macroglobuline and lypoproteine systems in the American mink (Neovison vison)

“…In the first publication, we described the group of markers Lpm1, Lpm2, Lpm3, Lpm4, and Lpm5, which was called the Lpm system (Lipo protein of mink), and low density lipoprotein allotype Ld1, which was classified with the Ld system (Belyaev et al, 1974;Baranov et al, 1975Baranov et al, , 1976Baranov et al, , 1978Savina, 1979, 1981). In the early 1970s, there was no data on the degree of polymorphism for both Lpm or Ld systems.…”

“…Mink blood samples were analyzed by the standard procedure using a set of alloantiserum reagents pre pared earlier by alloimmunization (Baranov et al, 1976(Baranov et al, , 1978. After a long break in experimental immu nogenetic studies , we managed to restore the activity of some antiserum reagents and analyzed the Lpm system for markers Lpm1, Lpm2, Lpm3, Lpm4, Lpm5, and Lpm10 (Baranov et al, 1976(Baranov et al, , 1978(Baranov et al, , 1984 and the Ld system for markers Ld1 and Ld2 Savina, 1979, 1981).…”

“…After a long break in experimental immu nogenetic studies , we managed to restore the activity of some antiserum reagents and analyzed the Lpm system for markers Lpm1, Lpm2, Lpm3, Lpm4, Lpm5, and Lpm10 (Baranov et al, 1976(Baranov et al, , 1978(Baranov et al, , 1984 and the Ld system for markers Ld1 and Ld2 Savina, 1979, 1981).…”

“…The frequencies of allogroups and respective hap lotypes were calculated and estimated by the conven tional immunogenetic methods with allowance for the characteristic features of the allogroup inheritance pattern (Baranov et al, 1978).…”

“…The study of two genetic systems, Lpm and Ld, in ani mals, which are classified with lipoproteins on the basis of their biochemical properties, a polymorphic Lpm (Lipo Protein of Mink) system with 14 allotypes (Lpm1-Lpm14), 11 allogroups, and, respectively, 11 haplotypes, was identified. In contrast, the system of low density lipoproteins with allelic markers Ld1 and Ld2 was monomorphic (Baranov et al, 1975;Baranov et al, 1978;Savina, 1979, 1988;Yermolaev et al, 1992).…”