Immunofluorescence of Green Monkey Kidney Cells Infected with Adenovirus 12 and with Adenovirus 12 Plus Simian Virus 40

Malmgren, Richard A.; Rabson, Alan S.; Carney, Paula; Paul, Frances J.

doi:10.1128/jb.91.1.262-265.1966

Cited by 35 publications

(15 citation statements)

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“…This restriction can be overcome by coinfection with simian virus 40 (SV40). In the restricted system, adenovirus 2 (Ad2) T antigen is produced (8,25), viral DNA is replicated (3,28), and viral RNA synthesis appears to be unaffected (9,10). The defect is thought to reside in the production of structural components of the adenovirion, which are found to be greatly reduced in the restricted system (2,11,15).…”

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confidence: 99%

Post-transcriptional restriction of human adenovirus expression in monkey cells

Eron¹

1975

J Virol

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Infection of the continuous simian cell lines CV-1 and BSC-1 with human adenovirus type 2 (Ad2) is abortive. However, the restriction of Ad2 reproduction in these cells can be overcome by increasing the Ad2 infectious dose or by coinfection with simian virus 40. Vero, another established simian cell line free of detectable endogenous simian virus 40 DNA, is not restricted in its ability to promote Ad2 growth even at low input multiplicities of Ad2 and in the absence of SV40 helper. The amount of structural Ad2 proteins in total cell extracts of enhanced BSC-1 cells is at least two orders of magnitude higher than that of unenhanced cells. In contrast, comparable quantities of Ad2 mRNA specifying these proteins are found in both the enhanced and the unenhanced cell. Both sets of mRNA can be translated in a cell-free system with equal efficiency.

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confidence: 99%

Post-transcriptional restriction of human adenovirus expression in monkey cells

Eron¹

1975

J Virol

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“…Subsequent studies showed that human adenoviruses undergo an abortive replicative cycle in AGMK cells (11). Under conditions of single I infection, early adenovirus antigens could be detected by immunofluorescence (11,25) from which it became apparent that the initial stages of the replicative cycle, involving adsorption, penetration, and uncoating of the infecting virions, are unaffected. Analyses of DNA extracted from singly and doubly infected cultures revealed that adenovirus DNA is synthesized during abortive infections (33,35).…”

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Biochemical Consequences of Type 2 Adenovirus and Simian Virus 40 Double Infections of African Green Monkey Kidney Cells

Friedman

Lyons

Ginsberg

1970

J Virol

102

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African green monkey kidney (AGMK) cells were nonpermissive hosts for type 2 adenovirus although the restriction was not complete; when only 3 plaqueforming units/cell was employed as the inoculum, the viral yield was about 0.1% of the maximum virus produced when simian virus 40 (SV40) enhanced adenovirus multiplication. The viral yield of cells infected only with type 2 adenovirus increased as the multiplicity of infection was increased. Type 2 adenovirus could

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“…Fluorescent antibody techniques and tests for reactivity and specificity of the sera have been described previously. 7 Determination of amylase: Transformed cell cultures and tumors developing in transplanted animals were homogenized (5 cc of buffered saline per gram of tissue); homogenates were assayed for amylase by the method of Van Loon. 15 Attempts to isolate uirus from transformed cells: LLE46 and SV40 transformed cells were trypsinized, suspended in one ml of growth media and placed in GMK and HEK culture tubes.…”

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confidence: 99%

In vitro neoplastic transformation of newborn hamster salivary-gland tissue by oncogenic DNA viruses

Wells

Rabson

Malmgren

et al. 1966

Cancer

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Explant cultures of newborn hamster salivary-glands were infected with 4 oncogenic DNA viruses: polyoma virus, human adenovirus 12, simian virus 40 (SV40) and the LLE46 strain of adenovirus 7 (the adenovirus 7-SV40 "hybrid").Only SV40 and LLE46 infected tissues underwent "transformation." Subcutaneous injection of SV40 transformed cells into irradiated adult hamsters produced fibrosarcomas. Subcutaneous injection of LLE46 transformed cells, however, produced tumors which were bimorphic with 2 distinct histological elements, one composed of small hyperchromatic undifferentiated cells resembling a n adenovirus type tumor a n d the other resembling a n SV40-induced sarcoma. No evidence of salivary-gland function as indicated by amylase production could be demonstrated in the transformed cultures or i n the tumors which they produced. N 1959 DAWE AND L A W~ OBSERVED THAT NEW-I born mouse salivary-glands, infected with polyoma virus and grown as organ cultures in vitro, developed morphologic characteristics of neoplasia. Vogt and Dulbeccolfi reported transformation of cellular morphology and growth characteristics of monolayer cultures of mouse and hamster embryo cells exposed in vitro to polyoma virus. They also were able to induce progressively growing neoplasms in adult hamsters by subcutaneously transplanting the transformed hamster embryo cells.Subsequently simian virus 40 (SV40), anFrom the Surgery and Pathologic Anatomy Branches,

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Immunofluorescence of Green Monkey Kidney Cells Infected with Adenovirus 12 and with Adenovirus 12 Plus Simian Virus 40

Cited by 35 publications

References 4 publications

Post-transcriptional restriction of human adenovirus expression in monkey cells

Post-transcriptional restriction of human adenovirus expression in monkey cells

Biochemical Consequences of Type 2 Adenovirus and Simian Virus 40 Double Infections of African Green Monkey Kidney Cells

In vitro neoplastic transformation of newborn hamster salivary-gland tissue by oncogenic DNA viruses

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