Biochemical Consequences of Type 2 Adenovirus and Simian Virus 40 Double Infections of African Green Monkey Kidney Cells

Friedman, Michael P.; Lyons, Michael J.; Ginsberg, Harold S.

doi:10.1128/jvi.5.5.586-597.1970

Cited by 102 publications

(71 citation statements)

References 42 publications

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“…The total counts per minute representing each viral DNA peak were tabulated. This procedure has been shown to extract efficiently both SV40 and adenovirus DNAs (8). The identities of the viral DNAs in the peak fractions were confirmed by DNA-DNA hybridization.…”

Section: Methodsmentioning

confidence: 85%

“…5) and similarly labeled at varying times after infection (4 to 36 h). As shown previously, this adenovirus MOI in monkey cells leads to viral DNA replication but little virus production in the absence of SV40 (2,8,26). After purification, samples of either SV40 or Ad5 DNA were precipitated with trichloroacetic acid and counted to determine the kinetics of viral DNA replication in singly infected cells.…”

Section: Kinetics Of Inhibition Of Sv40 Dna Synthesis During Coinfectmentioning

confidence: 82%

“…Although the reason for this reduction in SV40 RNA is not clear, the similar ratios of SV40 nuclear RNA to cytoplasmic RNA with and without Ad5 suggest that the reduction was not due to a processing step which affected transport and resulted in accumulations of SV40 RNA in the nuclei of coinfected cells. DISCUSSION The enhancement of adenovirus growth in monkey kidney cells has been the subject of numerous studies (1,8,25,32). A less well understood corollary of the interaction between these two viruses is the inhibition of SV40 DNA replication (8,32).…”

Section: Kinetics Of Inhibition Of Sv40 Dna Synthesis During Coinfectmentioning

confidence: 99%

“…The efficiency of adenovirus reproduction can be enhanced as much as 3 logs by coinfection (or preinfection) of the monkey cells with simian virus 40 (SV40). Although mechanism of this enhancement is not clear, it appears to depend on the expression of an early SV40 gene product (8,17). Furthermore, several experiments have shown that the entire early SV40 gene region need not function.…”

mentioning

confidence: 99%

“…A less intensively studied corollary to the enhancement of adenovirus growth is the inhibition of SV40 DNA synthesis, which occurs during coinfection of monkey cells (8,32). The cause of this block to SV40 replication is not clear.…”

mentioning

confidence: 99%

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Adenovirus regulation of simian virus 40 macromolecular synthesis

Goldman¹,

Khoury²

1980

J Virol

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At low multiplicities of infection (1 to 5 PFU/cell), human adenoviruses grew inefficiently in monkey kidney cells as the result of a late transcriptional and/or translational defect. Through an unknown mechanism, coinfection with simian virus 40 (SV40) could overcome this block. Coincident with the onset of adenovirus DNA replication in coinfected monkey cells, however, there was a marked inhibition of SV40 DNA replication. Since this inhibition in SV40 DNA synthesis occurred in the presence of normal SV40 transcription and protein synthesis, we suggest that adenovirus competes with SV40 for molecules specifically required for DNA synthesis (e.g., SV40 T-antigen). At high multiplicities of adenovirus infection (50 PFU/cell), adenoviruses could grow efficiently in monkey kidney cells. In cells coinfected under these conditions, SV40 transcription was generally inhibited, as shown by a decrease in nuclear and cytoplasmic SV40 mRNA.

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Section: Methodsmentioning

confidence: 85%