IMMUNOEXPRESSION OF p53 PROTEIN AND PROLIFERATING CELL NUCLEAR ANTIGEN IN PENILE CARCINOMA

Martins, Antônio Carlos Pereira; Faria, Simone Aparecida dos Santos Conceição; Cologna, Adauto José; Suaid, Haylton Jorge; Tucci, Sílvio

doi:10.1097/00005392-200201000-00022

Cited by 10 publications

(13 citation statements)

References 14 publications

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“…I). The estimated visual intensity ofp53 and Ki67 immunostaining was graded into three categories based on p53 and Ki67 index: negative when p53 or Ki67 index was <5% (group 0), weak when the index was >5% and < 50% (group I), strong when >50% (group II) (19)(20)(21).…”

Section: Tissue Samplesmentioning

confidence: 99%

Detection of Human Papillomavirus DNA, P53 and KI67 Expression in Penile Carcinomas

Gentile

Vicini

Giacomelli

et al. 2006

Int J Immunopathol Pharmacol

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Our study is aimed at evaluating the presence of p53 and Ki67 expression by immunohistochemistry in a series of 11 paraffin-embedded penile carcinomas. We also investigated the presence of Human Papillomavirus (HPV) DNA in these tumours and performed an accurate typing by DNA sequencing on positive samples. Immunohistochemistry (IHC) was performed with the anti-p53 and Ki67 mouse monoclonal antibodies. DNA extracted from small sections of each specimen was submitted to amplification with HPV specific general primers; PCR products ofthe proper length were purified and sequenced. IHC demonstrated nuclear accumulation of mutated p53 and Ki 67 expression in 10/11 tumour samples (90.9%). The prevalence of HPV DNA was 72.7%; the most prevalent type was HPV16. Sequencing analysis revealed the presence of HPV53 (12.5%), HPV18 (25%) and HPV16 (62.5%). Out of the p53 or Ki67 positive carcinomas the percentage ofHPV positives was 80% and 70% respectively. Our results indicate that penile carcinoma is frequently associated to high risk HPV and with diffuse p53 and Ki67 expression.Invasive cancer of the penis is a relatively uncommon disease in Western countries (0.I-0.9 per 100,000 males in Europe and0.7-0.9 per 100,000 males in the USA) whereas the highest incidence occurs in developing countries; in someareasofAsia, Africa and South America the incidence of penile carcinoma is significantly higher, reaching 19per 100,000 males. In these countries, penile carcinoma accounts for as much as 10-20% of male cancer(I). This carcinoma spreads locally to regional lymph nodes which are often surgically treated. This procedure has a high morbidity rate(30-90%) (2); thusthe majorissue in treating penile cancer involves the decision of which patient should or should not be submitted to lymph node. dissection. Many groups have identified prognostic factors for the incidence of lymph node metastases to avoid surgical morbidity such as: tumour thickness, histopathological grade, and venous and lymphatic embolization by neoplastic cells (3).In recent years human Papillomaviruses (HPVs) have been identified as possible etiological agents for the disease but thecorrelationbetweenthe viral DNA presence and the prognosis is stilluncertain (4 human papillomavirus (HPV) in cervical carcinomas and the association between HPV and cervical cancer has now been ascertained to reach 99.7% (5). The overall frequency of HPV DNA detection in carcinoma of the penis is far lower, nonetheless the reported prevalence of HPV is highly variable, from 15% to 70%, depending on the sensitivity of the detection method and on the tumour type (6-8). Moreover, only a few studies have analyzed penile carcinogenesis considering both the HPV status and the histological differentiation.The progress in molecular biology techniques makes it possible to analyse several neoplastic diseases by the identification of oncogene activation and/or tumour suppressor gene inactivation. The tumour suppressor gene p53 is located in the short arm of chromosome 17 and has been implicat...

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Section: Tissue Samplesmentioning

confidence: 99%

Detection of Human Papillomavirus DNA, P53 and KI67 Expression in Penile Carcinomas

Gentile

Vicini

Giacomelli

et al. 2006

Int J Immunopathol Pharmacol

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“…Furthermore, tumour marker profiles including several markers with different biological roles are more helpful in assessing a patient's prognosis. Recent evidence suggested that p53 and epithelial cadherin (E-cadherin) might be promising predictors of metastasis in penile cancer [13][14][15]. In the present study we examined various molecular markers (p53, Ki-67, E-cadherin, and matrix metalloproteinase-9, MMP-9) in patients with primary penile cancer to investigate their prognostic value for LNM and cancer-specific survival (CSS).…”

Section: Introductionmentioning

confidence: 98%

The prognostic significance of p53, Ki‐67, epithelial cadherin and matrix metalloproteinase‐9 in penile squamous cell carcinoma treated with surgery

et al. 2007

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OBJECTIVE To evaluate the prognostic significance of p53, Ki‐67, epithelial cadherin (E‐cadherin) and matrix metalloproteinase‐9 in primary penile cancer, as the presence of lymph node metastasis and long‐term survival are hard to define in penile squamous cell carcinoma. PATIENTS AND METHODS Paraffin‐embedded primary tumour samples were obtained from 73 Chinese patients who had penile amputation and regional lymphadenectomy. The expression of molecular markers was determined by immunohistochemistry. Logistic regression was used to identify factors associated with lymph node metastasis, and a Cox proportional‐hazards model was used to measure cancer‐specific survival (CSS). RESULTS Thirty (41%) patients presented with nodal disease and the 3‐year CSS rate for all patients was 72%. Lymph node metastasis was significantly correlated with tumour stage, histological grade, lymphatic and vascular embolization, and the expression of p53, Ki‐67 and E‐cadherin. By multivariate analysis, tumour embolization and the expression of p53 were independent predictors of metastasis. Survival analysis showed that the expression of p53 was an independent prognostic factor for CSS. In stage T1 tumours, high expression of p53 was significantly associated with metastasis and poor survival. CONCLUSION Lymphatic and vascular embolization, and p53 immunoreactivity, are helpful in establishing the probability of lymph node metastasis. The expression of p53 is an independent predictor of CSS in Chinese patients with penile cancer. In stage T1 tumours, p53 staining is an important variable determining the prognosis and treatment outcome.

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“…It implies that, besides venous invasion, other factors might also participate in the process of liver metastasis [30][31][32] . PCNA is a kind of non-histone nuclear polypeptide with 36 000 molecular weight, as an assistant protein to DNA polymerase, its content could reflect the degree of cell proliferation [33][34][35][36][37] . Previous studies showed that there was a significant relationship between PCNA labelling and degree of malignancy, vessel invasion, distant metastasis and prognosis [38][39][40][41][42] .…”

Section: Discussionmentioning

confidence: 99%

Expression of PCNA and CD44mRNA in colorectal cancer with venous invasion and its relationship to liver metastasis

Yue¹

2003

WJG

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AIM:To investigate the expression of proliferating cell nuclear antigen (PCNA) and CD44mRNA in colorectal cancer with venous invasion and its relationship with liver metastasis. METHODS:Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of PCNA and CD44mRNA in 31 cases of colorectal cancer with venous invasion. RESULTS:Positive expression rates of PCNA and CD44mRNA in colorectal cancer were higher than those without liver metastasis (P<0.05 and P<0.01). In case of colorectal cancer with liver metastasis, strongly positive rates of PCNA and CD44mRNA were 94.1 % and 70.6 %, respectively, significantly higher than those without liver metastasis. There was a positive relationship between the expressions of PCNA and CD44mRNA (r=0.67, P<0.05). CONCLUSION:Detection of PCNA and CD44mRNA expression in colorectal cancer may be useful for evaluating liver metastasis of cancer cells.Yue SQ, Yang YL, Dou KF, Li KZ. Expression of PCNA and CD44mRNA in colorectal cancer with venous invasion and its relationship to liver metastasis.

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IMMUNOEXPRESSION OF p53 PROTEIN AND PROLIFERATING CELL NUCLEAR ANTIGEN IN PENILE CARCINOMA

Cited by 10 publications

References 14 publications

Detection of Human Papillomavirus DNA, P53 and KI67 Expression in Penile Carcinomas

Detection of Human Papillomavirus DNA, P53 and KI67 Expression in Penile Carcinomas

The prognostic significance of p53, Ki‐67, epithelial cadherin and matrix metalloproteinase‐9 in penile squamous cell carcinoma treated with surgery

Expression of PCNA and CD44mRNA in colorectal cancer with venous invasion and its relationship to liver metastasis

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