Immunoexpression of c-erbB-2 and p53 in benign and malignant salivary neoplasms with myoepithelial differentiation.

Abstract

Aim-To evaluate whether the immunoexpression of c-erbB-2 and p53 is involved in the pathogenesis and progression of salivary tumours with myoepithelial differentiation. Methods-233 tumours from 211 patients were studied. These included 76 primary and 24 recurrent adenocarcinomas (polymorphous low grade adenocarcinoma, 13; epithelial-myoepithelial carcinoma, 19; adenoid cystic carcinoma, 56; and basal cell adenocarcinoma, 12) and 133 pleomorphic adenomas and myoepitheliomas, 96 being primary and the remaining recurrent tumours. All cases were formalin fixed and paraffin wax embedded. A StrepABC peroxidase method and polyclonal c-erbB-2 and p53 specific antisera were used.Results-Cell membrane staining of c-erbB-2 was not found in any benign or malignant tumour. There was p53 protein accumulation in one primary and one recurrent pleomorphic adenoma and in 10 adenocarcinomas (polymorphous low grade adenocarcinoma, one; epithelialmyoepithelial carcinoma, one; adenoid cystic carcinoma, five; and basal cell adenocarcinoma, three), three of them being recurrences. Conclusions-The c-erbB-2 and p53 proteins are not involved in the pathogenesis of pleomorphic adenoma and myoepithelioma and do not constitute biomarkers in assessing the risk of recurrence. c-erbB-2 is not involved in the genesis of low grade salivary neoplasia with myoepithelial differentiation. The percentage ofthis type of neoplasia with p53 accumulation is low (10%) and does not appear to be related to tumour recurrence.

Adenoid cystic carcinoma: A retrospective clinical review

“…Staining was predominantly in tubular and cribriform areas, and was associated with shorter disease-free intervals, although the authors question the significance of the finding due to the small numbers of positive cases and the focal nature of the reactivity. Two other studies [14,15] looking at HER-2/neu overexpression in salivary neoplasms could not demonstrate any membrane staining at all among the ACCs subsets in their series. The first group [14] had 56 ACCs in their study, whereas the second study [15] had 8.…”

“…Two other studies [14,15] looking at HER-2/neu overexpression in salivary neoplasms could not demonstrate any membrane staining at all among the ACCs subsets in their series. The first group [14] had 56 ACCs in their study, whereas the second study [15] had 8. The reasons for these differences are not completely apparent at this time.…”

“…Some markers evaluated using immunohistochemistry include the epidermal growth factor receptor (EGF-R), HER-2, HER-3, p53, and proliferating cell nuclear antigen (PCNA) [10][11][12][13][14][15][16]. The resultant data for ACC have not been concordant.…”

Intraoral Mucoepidermoid Carcinoma of Salivary Glands: Lack of Association Among Clinicopathological Features and Immunoexpression of c-erbB-2 in 29 Cases

“…There have been suggestions that the overexpression of c-erbB-2 on salivary gland tumors is related with histogenesis of these lesions (Glisson et al, 2004;Rivière et al, 1991;Gibbons et al, 2001, Rosa et al, 1997. MEC is composed of varying proportions of mucous, epidermoid, columnar, intermediate and clear cells (Auclair et al) and is though to arise from the salivary excretory duct (Ellis et al).…”

Inmunohistochemical Expression of PCNA, p53 and bcl-2 in Pleomorphic Adenomas

“…Mutation in the tumor suppressor gene p53 is the most common genetic modification found in malignant oral tumors. Additionally, numerous studies involving proliferating cell nuclear antigen (PCNA) and the bcl-2 protein have been conducted to determine the processes related to cell proliferation and, consequently, the susceptibility of some tumors to malignant transformation (Ohtaké et al;Rosa et al, 1997;Yanez et al, 1999;.…”