Immunodiagnostics of Malignant Disease VI. Electrophoretic Mobility Test (EMT) in Malignant Melanoma

Abstract

The electrophoretic mobility test (EMT) is an in vitro assay for demonstrating cellular immunity. In the presence of tumor antigens lymphocytes of tumor patients liberate lymphokines, which reduce the charge of indicator particles resulting in a measurable reduction of their electrophoretic mobility. Lymphocytes of 174 patients were tested by EMT. The antigens used were a basic myelin protein termed encephalitogenic factor (EF) and a 3M KCl extract from melanoma tissue. In 91% of the cancer patients there was a positive lymphocyte response. In contrast to this the controls and non-malignant diseases showed a positive result in only 8.7% of the cases. Using the 3M KCl extract from melanoma tissue as tissue as antigen 1 of the benign controls, 3 patients with non-malignant diseases and none of the 49 patients with malignant diseases reacted positively, whereas in the melanoma group 86% showed a positive lymphocyte response. The results show the possibility of demonstrating tumor specific immune reaction in the EMT.

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

“…6). This seems to be comparable to the slowing effect of supernates derived from incubations with other basic protein mixtures as shown by other investigators [6,11,13]. 9.…”

Cell electrophoresis for diagnostic purposes. I. Diagnostic value of the electrophoretic mobility test (EMT) for the detection of gynaecological malignancies

“…Several centres (using EF, CaBP or the KCI extracts as "antigens") confirmed the results of MEM (Pritchard et al, 1972(Pritchard et al, , 1973Preece & Light, 1974;Field, 1976;Irmscher et al, 1975;Klausch et al, 1975;Meyer-Rienecker et al, 1975;Jenssen et al, 1976a,b;Nowak et al, 1976;Klausch et al, 1977;Light & Preece, 1977;Muller et al, 1977;Gunther et al, 1978), MOD-MEM (Pritchard et al, 1976) and EMT (Jenssen & Shenton, 1975;Lampert et al, 1977;Shenton et al, 1977;Douwes et al, 1978;Dyson & Corbett, 1978;Tautz et al, 1978;Ritter & Oehme, 1978;Kreienberg et al, 1979;B6gelspacher et al, 1980), though the procedures have not gained universal recognition (see also Bagshawe, 1973;leading article in Nature, 1973;editorial in Lancet, 1976;Bagshawe, 1977;Moore & Lajtha, 1977;Moore, 1978).…”

Immundiagnostik maligner Erkrankungen

“…In Abwandlung zu früheren Beschreibungen haben wir den Testansatz etwas modifiziert durchgeführt [7,8,9]. 0,5 ml der Lymphozytensuspension wurde inkubiert mit 0,5 ml EF-Lö-sung, welche so eingestellt war, daß sich unter Zugabe von 2,0 ml Hankslösung 66 pg EF/ml befanden (Abbildung 3 …”