Immunocytochemical study of the lung of domestic fowl and pigeon: endocrine cells and nerves

López, Jesús García; Barrenechea, M. A.; Burrell, Marı́a A.; Sesma, P.

doi:10.1007/bf00304615

Cited by 5 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These cells within the raceme also stained for serotonin (Fig. 2J–L), a characteristic feature of neuroendocrine cells in birds as well as in mammals (Salvi & Renda, 1992; Lopez et al. 1993; Adriaensen et al.…”

Section: Resultsmentioning

confidence: 89%

“…In his silver‐stained material, Groth could not identify a close relation of the Endkolben to any kind of ‘helper‐cell’ and claimed all the sensory endings were of a non‐encapsulated nature. We would agree with this last conclusion, but our techniques of labelling the innervating fibre, combined with SV2 and Hoechst staining, allowed us to show (1) that the racemes (Groth's Endkolben) are innervated by the vagus nerve; (2) that cells are characteristically associated with the vagal nerve terminals; (3) that these cells form a compact aggregate and are SV2‐positive, suggesting the presence of neurosecretory vesicles (Buckley & Kelly, 1985); and (4) that the SV2‐positive cells within the raceme are also positive for serotonin, a feature that is characteristic of neuroendocrine cells in other parts of the respiratory tract of birds (Salvi & Renda, 1992; Lopez et al. 1993; Adriaensen et al.…”

Section: Discussionmentioning

confidence: 99%

“…To visualize afferent and efferent components of the vagus nerve in the brain, and vagally innervated structures in the air sacs, we used an antibody against CTB (see below). In addition, to identify cellular and subcellular components of the vagally innervated structures in the air sac, we used antibodies against a synaptic vesicle protein SV2 (Buckley & Kelly, 1985), which labels both synaptic and neurosecretory vesicles; serotonin, which is characteristically found in avian neuroendocrine cells (Salvi & Renda, 1992; Lopez et al. 1993; Adriaensen et al.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Vagal innervation of the air sacs in a songbird, Taenopygia guttata

2004

View full text Add to dashboard Cite

The air sacs of birds are thin-walled chambers connected to the lung that act as bellows in the ventilatory mechanism. Physiological evidence exists to suggest that they may contain receptors that are innervated by the vagus nerve, but no morphological study has examined the vagal innervation of these putative structures. To do this, we injected the cervical vagus nerve with choleragenoid and examined the innervation of the air sacs using light and confocal microscopy. We identified vagally innervated structures in the air sac wall that resemble the neuroepithelial bodies (NEBs) described in the airways of many vertebrates. Although NEBs have been proposed to have a dual chemoreceptive and mechanoreceptive role, their specific function in the air sacs of birds remains unclear.

show abstract

Section: Resultsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Vagal innervation of the air sacs in a songbird, Taenopygia guttata

2004

View full text Add to dashboard Cite

show abstract

Immunocytochemical survey of the neuropeithelial endocrine system in the respiratory tract of the Tokyo salamander, Hynobius neubulosus tokyoensis Tago

et al. 1994

View full text Add to dashboard Cite

The epithelial lining of the respiratory tract of urodeles has been shown to harbor an innervated system of neuroepithelial endocrine (NEE) cells. Even between phylogenetically closely related species, large differences have been reported in the appearance and chemical coding of the NEE system. Although urodeles are well suited for the purpose, none of the prior studies have provided an immunocytochemical survey of the NEE system in all parts of the respiratory tract. In the present study, many bioactive substances and a general marker were immunocytochemically demonstrated in serial sections of the entire respiratory tract of the Tokyo salamander, Hynobius nebulosus tokyoensis, a species in which neuroepithelial bodies (NEBs) were previously characterized at the electron microscopic level. In the current study, serotonin-immunoreactive solitary NEE cells were observed in variable numbers in the larynx, in all parts of the trachea, and in areas of the lungs covered with ciliomucous epithelium. Serotonin-containing NEBs, however, were detected in small cranial areas of the lung only. Solitary NEE cells were seen in the trachea and lungs of H. nebulosus tokyoensis by immunocytochemical staining for somatostatin, calcitonin, calcitonin gene-related peptide, and bombesin, but the number, localization, and appearance of the labeled NEE cells differed considerably. Only calcitonin-like immunoreactivity was also noted in some NEB-like cell clusters in the cranial parts of the lungs. Unlike many other vertebrates, neuron specific enolase was found to be a poor marker for the NEE system in the salamander species used in this investigation. It may be concluded that the NEE system of H. nebulosus tokyoensis contains at least five different bioactive substances.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract