Immunocytochemical survey of the neuropeithelial endocrine system in the respiratory tract of the Tokyo salamander, Hynobius neubulosus tokyoensis Tago

Gomi, Tatsuya; Kikuchi, Yuka; Adriaensen, Dirk; Timmermans, Jean‐Pierre; Groodt-Lasseel, M.H.A. de; Kimura, Akihiko; Naruse, H.; Ishikawa, Yoshiharu; Kishi, Kazuki; Scheuermann, D.W.

doi:10.1007/bf00269573

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…In the trachea of the reptile Podarcis hispanica, serotonin-positive endocrine cells were not found (Beorlegui et al, 1994). However, in the Tokyo salamander, Hynobius nebulosus tokyoensis, these cells were reported to be present (Gomi et al, 1994).…”

Section: Discussionmentioning

confidence: 96%

Light microscopic and immunohistochemical study of the trachea of the broad-snouted caiman (Caiman latirostris)

Santos¹,

Figueiredo²,

Teixeira³

et al. 2011

Vet. Med. - Czech

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ABSTRACT:The purpose of this study was to examine the tracheal structure of the crocodile Caiman latirostris using light microscopy, histochemical and immunocytochemical techniques. The tracheal epithelium of C. latirostris consists of a ciliated pseudostratified columnar epithelium with goblet cells. The respiratory epithelium also includes endocrine cells immunoreactive to serotonin. The histochemical techniques demonstrated the presence of neutral and sulphated mucins secreted by goblet cells. The lamina propria consists of connective tissue with many reticular fibres. The elastic fibres are interspersed among collagen bundles, forming the border between the mucosa and the submucosa. The submucosal layer consists of connective tissue similar to that found in the lamina propria. Serous or mucous glands were not observed. The predominant characteristic in the adventitia is the presence of an incomplete hyaline cartilage ring, in the form of a circle. Dense connective tissue fills the space between the extremities of each cartilage ring. Serotonin-immunoreactive cells frequently had an apical cytoplasmic process directed towards the lumen, and were therefore classified as open type. The α-actin immunohistochemistry revealed smooth muscle cells only in blood vessel walls, confirming the absence of a tracheal muscle.

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Section: Discussionmentioning

confidence: 96%

Light microscopic and immunohistochemical study of the trachea of the broad-snouted caiman (Caiman latirostris)

Santos¹,

Figueiredo²,

Teixeira³

et al. 2011

Vet. Med. - Czech

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“…A limited number of bombesin-IR single cells were found at the base of ciliated epithelium in the lungs of lower vertebrates, e.g., T. alpestris, H. nebulosus tokyoensis and R. temporaria (Cutz et al, 1986;Gomi et al, 1994;Bodegas et al, 1995). Cutz et al (1986) did not observe any bombesin immunoreactivity in the lung epithelium of B.…”

Section: Discussionmentioning

confidence: 86%

“…PNECs containing 5-HT were found in the lungs of urodelan amphibians, including A. tigrinum and H. nebulosus tokyoensis (Goniakowska-Witalinska et al, 1992;Gomi et al, 1994), and anuran amphibians, including B. orientalis, R. nigromaculata and R. temporaria (Wasano & Yamamoto, 1979;Goniakowska-Witalinska et al, 1990;Bodegas et al, 1995). Investigations have shown that these cells are located in the bronchial and bronchiolar ciliated epithelium of mammals, and at the base of the ciliated epithelium both in primary and secondary septa of the lungs of lower vertebrates, such as P. scripta elegans, B. orientalis, and H. nebulosus tokyoensis Goniakowska-Witalinska et al, 1990;Gomi et al, 1994). The localisation and morphology of 5-HT-IR PNECs in the lungs of R. ridibunda was generally similar to those in the other animals.…”

Section: Discussionmentioning

confidence: 99%

The responses of pulmonary neuroendocrine cells in the lungs of Rana ridibunda to different experimental conditions

Öztay¹

2006

Animal Biol

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The study investigated the localisation and immunohistochemistry of neuroendocrine cells (PNECs) and their behaviour in the lungs of Rana ridibunda under experimental conditions and compared with control lungs. Serotonin-and bombesin-immunoreactive (IR) PNECs were observed as solitary cells or clusters of cells at the base of ciliated epithelium on the dilated apical parts of primary septa where incoming air first arrives, and in the respiratory epithelium. Serotonin-IR PNECs were predominantly present in the ciliated epithelium while bombesin-IR PNECs were found in the ciliated and respiratory epithelium. Additionally, bombesin-IR nerve fibres were found in the epithelium and among smooth muscle cells in the connective tissue. In frogs kept in a dry aquarium, serotoninand bombesin-IR PNECs were characterised by various stages of secretion. Serotonin-IR PNECs released most of their secretory material, while bombesin-IR PNECs were stimulated for secretion. Moreover, bombesin secretions were raised. In conclusion, the cells were identified as members of the pulmonary epithelial endocrine system and can contribute to the pulmonary biology through a paracrine/endocrine pathway in the lungs of R. ridibunda.

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“…The arytenoid portion (i.e. from LT1 to LT4) in the red‐bellied newt occupies approximately the cranial two‐thirds of the laryngotrachea, whereas that in the Tokyo salamander is located in the cranial one‐third of the laryngotrachea (Gomi et al ., 1994; Kimura et al ., 2000). The epithelium of the laryngotrachea of the red‐bellied newt is also slightly different from that of the Tokyo salamander.…”

Section: Discussionmentioning

confidence: 99%

“…The following bioactive substances have been found in the NEE cells, as well as the NEBs, of amphibians: serotonin (5‐hydroxytriptamine; Cutz et al ., 1986; Scheuermann et al ., 1989; Goniakowska‐Witalinska et al ., 1992), bombesin (Cutz et al ., 1986; Gomi et al ., 1994), enkephalin (Goniakowska‐Witalinska et al ., 1992), substance P (SP; Adriaensen & Scheuermann, 1993), somatostatin (Gomi et al ., 1994), calcitonin (Gomi et al ., 1994), calcitonin gene‐related peptide (CGRP; Gomi et al ., 1994) and neuron‐specific enolase (Gomi et al ., 1994). Serotonin was detected in a larger number of NEE cells than any other bioactive substance in the salamanders (Scheuermann et al ., 1989; Gomi et al ., 1994).…”

Section: Introductionmentioning

confidence: 99%

Structure of the respiratory tract of the red-bellied newt Cynops pyrrhogaster, with reference to serotonin-positive neuroepithelial endocrine cells

et al. 2005

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To investigate the distribution of serotonin-positive neuroepithelial endocrine (NEE) cells in the respiratory tract of the red-bellied newt Cynops pyrrhogaster, serial sections of the respiratory tract were stained using immunohistochemical methods for serotonin, a periodic acid-Schiff method and hematoxylin-eosin staining. The respiratory tract of the red-bellied newt consisted of a tubular laryngotrachea and two pulmonary sacs. Serotonin-positive solitary NEE cells were distributed widely throughout the ciliomucous epithelium of the laryngotrachea. The innervated clusters of NEE cells, termed neuroepithelial bodies, were rarely found in the caudal portion of the laryngotrachea. The density of NEE cells was high in the middle portion of the laryngotrachea, particularly in the region surrounded by a striated laryngeal sphincter, and the densities were low cranially towards the aditus laryngis and caudally in the pulmonary sacs, the epithelium of which consisted of both ciliomucous epithelial cells and non-ciliated respiratory epithelial cells or pneumocytes. The NEE cells were not observed in the vicinity of non-ciliated respiratory epithelial cells. The morphology and distribution of serotonin-positive NEE cells in the respiratory tract of the red-bellied newt were similar to those described in mammals, including humans. The close localization of NEE cells with ciliary and mucous cells and the distribution of NEE cells in the laryngotrachea suggest that NEE cells play important roles in the defense and repair of the ciliomucous epithelium, such as increasing mucous secretion, ciliary beat frequency and the proliferation of epithelial cells.

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Immunocytochemical survey of the neuropeithelial endocrine system in the respiratory tract of the Tokyo salamander, Hynobius neubulosus tokyoensis Tago

Cited by 12 publications

References 33 publications

Light microscopic and immunohistochemical study of the trachea of the broad-snouted caiman (Caiman latirostris)

Light microscopic and immunohistochemical study of the trachea of the broad-snouted caiman (Caiman latirostris)

The responses of pulmonary neuroendocrine cells in the lungs of Rana ridibunda to different experimental conditions

Structure of the respiratory tract of the red-bellied newt Cynops pyrrhogaster, with reference to serotonin-positive neuroepithelial endocrine cells

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