IMMUNOCYTOCHEMICAL LOCALIZATION OF a 19S Γ1 MACROGLOBULIN COLD HAEMAGGLUTININ

Curtain, C. C.; Baumgarten, A.

doi:10.1038/icb.1965.12

Cited by 6 publications

(4 citation statements)

References 3 publications

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“…demonstrated IgMK in some 10% of the tumour cells in each case. Together with previous fluorescent studies (Curtain & Baumgarten, 1965;Van Furth & Diesselhoff den Dulk, 1966;Marmont & Damasio, 1967) this evidence suggests that lymphoma can truly arise from the CA monoclone. In a few othcr cases (four among 57 patients with monoclonal IgM associated with R.E.S.…”

Section: Discussionsupporting

confidence: 70%

Immunoglobulins in Chronic Cold Haemagglutinin Disease

Cooper

Hobbs

1970

Br J Haematol

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Summary. Total serum IgM levels in 32 patients with chronic cold haemagglutinin disease ranged from 100 to 2400 mg/100 ml; follow‐up in nine patients showed slowly progressive increases. The excess IgM was mostly cold agglutinin and although there was considerable variation in serologic activity on a molecular basis, specific lytic activity against enzyme‐treated red cells was very similar. The cold agglutinin IgM seemed well produced and closely parallel to normal IgM; few 7S units and little Bence‐Jones proteinuria were detected. Non cold‐agglutinin IgM levels were mostly normal, but 31% of patients showed subnormal levels of IgA or IgG especially when the IgM level was greater than 1000 mg/100 ml.

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Section: Discussionsupporting

confidence: 70%

Immunoglobulins in Chronic Cold Haemagglutinin Disease

Cooper

Hobbs

1970

Br J Haematol

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“…Some such treated patients showed 'induction' of faster growing tumours, so cytotoxic drugs should be reserved until all else has failed. Many of the cold haemagglutinin-producing cells are in the spleen (Curtain and Baumgarten, 1965) so splenectomy, like plasmapheresis, lowers the serum IgM level but only temporarily (Worlledge et al, 1968). Our concept in this subgroup is that because the paraprotein has antibody activity (and offers a special area in which to study the binding site of a human monoclonal antibody) the patient usually presents and dies long before any underlying tumour can be found clinically.…”

mentioning

confidence: 99%

IgM paraproteins.

Hobbs¹,

Carter²,

Cooke³

et al. 1975

Journal of Clinical Pathology

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“…There are conflicting reports concerning the ability of one cell to produce antibody of two spedficities or immunoglobulins of two different groups (7)(8)(9)(10)(11)(12)(13)(14)(15)(16). The present data suggest that at least one-fourth of the peripheral lymphocytes carry or have the capacity to synthesize molecules of the three major immunoglobulin groups (IgA, IgG, and IgM) and the remaining three-fourths carry or have the capacity to produce molecules of two immunoglobulin groups (IgG and IgM).…”

Section: Discussionmentioning

confidence: 99%

Studies on Rabbit Lymphocytes in Vitro

Sell

1967

The Journal of Experimental Medicine

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The peripheral lymphocytes of the rabbit may be stimulated in vitro to transform into immature blast cells by sheep antisera to rabbit immunoglobulin G (IgG) and to rabbit IgG subunits (L chain, H chain, Fab piece, Fc piece), suggesting that the peripheral lymphocyte contains IgG molecules (1). Studies on lymphocytes from newborn rabbits using rabbit anti-allotype sera (2) and preincubation of lymphocytes prior to stimulation with anti-allotype (3) sera indicate that the IgG molecules present in lymphocytes are produced by these cells and are not obtained from the environment.Recent studies by Van F u r t h e t al. (4) demonstrate that cultures of the peripheral lymphocytes of humans are capable of producing immunoglobulins of the three major classes; i.e., IgG, IgA, and IgM. The studies to be described in the present report were designed to determine if antisera specific for rabbit IgA and IgM would be capable of inducing blast transformation and DNA synthesis in peripheral lymphocyte cultures. The results indicate that anti-IgM is as effective as anti-IgG in stimulating lymphoblast transformation and that anti-IgA is also stimulating, although not as effective as the other antisera. Materials and MethodsProduction of Antisera.--Anti-lgA: Rabbit immunoglobulin A was obtained from the colostrum of newly delivered does by chemical precipitation of whey and Sephadex G-200 fractionation (5). The preparation was tested by immunoelectrophoresis against sheep and goat polyvalent antisera to rabbit whole serum and only IgA was detectable. Each of two sheep was injected intramuscularly in the left shoulder and flank with 3 mg of IgA protein in 0.5 ml of saline emulsified with an equal volume of complete Freund's adjuvant (control 478918,

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IMMUNOCYTOCHEMICAL LOCALIZATION OF a 19S Γ1 MACROGLOBULIN COLD HAEMAGGLUTININ

Cited by 6 publications

References 3 publications

Immunoglobulins in Chronic Cold Haemagglutinin Disease

Immunoglobulins in Chronic Cold Haemagglutinin Disease

IgM paraproteins.

Studies on Rabbit Lymphocytes in Vitro

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